鱼源温和气单胞菌对氨基糖苷类和四环素类抗生素的耐药性分析

doi:10.16431/j.cnki.1671-7236.2018.04.028

Abstract

Abstract:

To detect the drug resistance characteristics of Aeromonas sobria to aminoglycosides and tetracyclines antibiotics,10 strains of Aeromonas sobria isolated from different fish were used for detection and analysis.Four aminoglycosides resistance genes (aph(3')-Ⅱa,ant(3″)-Ⅰa,aac(6')-Ⅰb and aac(3)-Ⅱa) and three tetracyclines resistance genes (tetA,tetC and tetM) were detected by PCR.The drug resistance phenotype of six antibiotics were analyzed by Kirby-Baner disc diffusion method.The results showed that the positive rate of the resistance genes of aminoglycosides aph(3')-Ⅱa,ant(3″)-Ⅰa,aac(3)-Ⅱa and aac(6')-Ⅰb in 10 strains of Aeromonas sobria were 20%,30%,0 and 20%,respectively.The positive rate of the resistance genes of tetracyclines tetA,tetC,tetM in 10 strains of Aeromonas sobria were 70%,20% and 60%,respectively.The analysis of drug resistant phenotype results showed that the strains were intermediary to streptomycin,and sensitive to gentamicin,kanamycin,doxycycline,minocycline,while the strains were resistant to tetracycline.The result implied that the identification of Aeromonas sobria had a certain degree of resistance to aminoglycoside and tetracycline antibiotics,which provided a reference for benefits further research of the resistance mechanism of Aeromonas sobria.

Key words: Aeromonas sobria; aminoglycosides; tetracyclines; resistance genotype; resistance phenotype

CLC Number:

S852.61

WU Kainian, WANG Li, LI Chenyang. Drug Resistance Analysis of Aminoglycosides and Tetracyclines in Aeromonas sobria Isolated from Fish[J]. , 2018, 45(4): 1059-1066.

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Drug Resistance Analysis of Aminoglycosides and Tetracyclines in Aeromonas sobria Isolated from Fish

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