牛源犬新孢子虫孕鼠感染模型的建立

doi:10.16431/j.cnki.1671-7236.2018.04.029

Abstract

Abstract:

To establish the model of pregnant mice infected by Neospora caninum (N.caninum) and understand the pathogenicity of N.caninum on pregnant mice,female BALB/c mice were inoculated intraperitoneally with different doses of N.caninum tachyzoites purified from Vero cells.Then these female BALB/c mice were maintained with male BALB/c mice in the same cage the next day.The clinical symptom and morbidity were observed daily.Basing on N.caninum Nc5 gene,PCR was performed to detect parasitic DNA in brain,liver,spleen and placenta from N.canium-infected mice,placenta wet weight and placental coefficients of pregnant mice were measured,too.The results revealed that the optimal dose to infect mice was 10⁵ tachyzoites.Pregnant mice behaved mental disorders,ataxia,and other clinical symptoms,even death simultaneously.Pathological changes such as congestion,hemorrhage,and enlargement occurred in the major organs of brain,liver,and spleen of model mice.Nc5 gene was detected successively in brain,liver,spleen and placenta of pregnant mice infected with parasites.With the increase of post-infection days,the weight of placenta and the fetus were increasing and the placental coefficient decreased gradually following infection.Significant differences were observed in placental weight and placental coefficient between model group and control group on 12,14 and 16 d (P < 0.05),respectively.The results indicated that the model of pregnant BALB/c mice infected by N.caninum was successfully established which laid the foundation for the study of pathogenesis of N.caninum.

Key words: bovine; Neospora caninum; pregnant mice; model

CLC Number:

S852.72⁺3

LIANG Yang, XIE Suzhu, LI Hang, ZHANG Ningning, JIA Lijun. Establishment of Model of Pregnant Mice Infected by Neospora caninum from Bovine[J]. , 2018, 45(4): 1067-1073.

References

[1] DUBEY J P,CARPENTER J L,SPEER C A,et al.Newly recognized fatal protozoan disease of dogs[J].Journal of the American Veterinary Medical Association,1988,192(9):1269-1285.
[2] KLAUCK V,MACHADO G,PAZINATO R,et al.Relation between Neospora caninum and abortion in dairy cows:Risk factors and pathogenesis of disease[J].Microbial Pathogenesis,2015,92:46-49.
[3] MOORE D,REICHEL M,SPATH E,et al.Neospora caninum causes severe economic losses in cattle in the humid pampa region of Argentina[J].Tropical Animal Health and Production,2013,45(5):1237-1241.
[4] KRISHNA R,XIA D,SANDERSON S,et al.A large-scale proteogenomics study of apicomplexan pathogens——Toxoplasma gondii and Neospora caninum[J].Proteomics,2015,15(15):2618-2628.
[5] TONKIN M L,CRAWFORD J,LEBRUN M L,et al.Babesia divergens and Neospora caninum apical membrane antigen 1 structures reveal selectivity and plasticity in apicomplexan parasite host cell invasion[J].Protein Science,2013,22(1):114-127.
[6] BRASIL A W,PARENTONI R N,FEITOSA T F,et al.Risk factors for Toxoplasma gondii and Neospora caninum seropositivity in buffaloes in Paraiba State,Brazil[J].Revista Brasileira de Parasitologia Veterinária,2015,24(4):459-463.
[7] BRUHN F R,DAHER D O,LOPES E,et al.Factors associated with seroprevalence of Neospora caninum in dairy cattle in Southeastern Brazil[J].Tropical Animal Health and Production,2013,45(5):1093-1098.
[8] OH J,LEE S H,LEE S J,et al.Detection of antibodies against Toxoplasma gondii in cattle raised in Gyeongbuk province,Korea[J].Journal of Food Protection, 2016,79(5):821-824.
[9] MACHACOVÁ T,AJZENBERG D,ZÁKOVSKÁ A,et al.Toxoplasma gondii and Neospora caninum in wild small mammals:Seroprevalence,DNA detection and genotyping[J].Veterinary Parasitology,2016,223:88-90.
[10] WILSON D J,ORSEL K,WADDING J,et al.Neospora caninum is the leading cause of bovine fetal loss in British Columbia,Canada[J].Veterinary Parasitology,2016,218:46-51.
[11] MARTINEZ B A,LEOTTI V B,BORBA M R,et al.Can hierarchical modeling improve our understanding of bovine abortion due to Neospora caninum infection?[J].Veterinary Parasitology,2017,237:77-82.
[12] HOSSEININEJAD M,MAHZOUNIEH M,SHAMS ESFANDABADI N.Neospora caninum suspects as one of the most important causes of abortion in large dairy farms in Isfahan[J].Iranian Journal of Parasitology,2017,12(3):408-412.
[13] ZHANG G,HUANG X,BOLDBAATAR D,et al.Construction of Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii infection in mice[J].Vaccine,2010,28(45):7243-7247.
[14] 李航,姜利建,刘晋宇,等.新孢子虫与弓形虫交叉抗原AMA1基因重组腺病毒的构建及免疫应答分析[J].中国畜牧兽医,2016,43(12):3336-3342. LI H,JIANG L J,LIU J Y,et al.Construction and analysis of the immune response of Neospora caninum and Toxoplasma gondii cross recombinant adenovirus antigen AMA1 vaccine[J].China Animal Husbandry & Veterinary Medicine,2016,43(12):3336-3342.(in Chinese)
[15] 陈千林,王振宝,吉尔格力,等.基于Nc2和Nc5基因的新孢子虫PCR方法的建立[J].中国畜牧兽医,2016,43(3):622-628. CHEN Q L,WANG Z B,JIERGELI,et al.Establishing the screening PCR diagnostic method of Neospora caninum based on Nc2 and Nc5 genes[J].China Animal Husbandry & Veterinary Medicine,2016,43(3):622-628.(in Chinese)
[16] JIA L J,ZHANG S F,LIU M M,et al.Isolation,identification,and pathogenicity of Neospora caninum China Yanbian strain[J].Iranian Journal of Parasitology,2014,9(3):394-401.
[17] ZHANG H,NISHIKAWA Y,YAMAGISHI J,et al.Neospora caninum:Application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice[J].Experimental Parasitology,2010,125(2):130-136.
[18] 龙烟朦,高鸿博,马磊,等.新孢子虫对小鼠致病力及在小鼠体内动态分布的研究[J].中国兽医杂志,2016,52(1):3-5. LONG Y M,CAO H B,MA L,et al.Virulence and dynamic distribution Neospora caninum in mice[J].Chinese Journal of Veterinary Medicine,2016,52(1):3-5.(in Chinese)
[19] 李文学,鲁承,杜秋明,等.新孢子虫速殖子灭活苗对沙鼠的免疫保护性试验[J].延边大学农学学报,2010,32(1):4-7. LI W X,LU C,DU Q M,et al.Neospora tachyzoites inactivated vaccine on gerbils rotective immunity test[J].Journal of Agricultural Science Yanbian University,2010,32(1):4-7.(in Chinese)
[20] 倪婷婷,伍生军,梁晚枫,等.猪附红细胞体小鼠感染模型建立条件的优化[J].中国畜牧兽医,2017,44(9):2761-2766. NI T T,WU S J,LIANG W F,et al.Optimization of establishment conditions of mouse model of Mycoplasma suis[J].China Animal Husbandry & Veterinary Medicine,2017,44(9):2761-2766.(in Chinese)
[21] 高倩,胡言青,唐懿挺,等.Wip1过表达转基因小鼠模型的制备与鉴定[J].中国畜牧兽医,2016,43(12):3094-3100. GAO Q,HU Y Q,TANG Y T,et al.Establishment and identification of Wip1 over-expressed transgene mice model[J].China Animal Husbandry & Veterinary Medicine,2016,43(12):3094-3100.(in Chinese)
[22] LONG M T,BASZLER T V.Fetal loss in BALB/c mice infected with Neospora caninum[J].Journal of Parasitology,1996,82(4):608-611.
[23] RAMAMOORTHY S,DUNCAN R,LINDSAY D S,et al.Optimization of the use of C57BL/6 mice as a laboratory animal model for Neospora caninum vaccine studies[J].Veterinary Parasitology,2007,145(3-4):253-259.
[24] HURKOVÁ-HOFMANNOVÁ L,VÁCLAVEK P,SKORIC M,et al.Multimammate rat (Mastomys natalensis),Tristram's jird (Meriones tristrami) and Wagner's gerbil (Gerbillus dasyurus) as laboratory models of acute neosporosis[J].Research in Veterinary Science,2007,82(3):377-381.

Establishment of Model of Pregnant Mice Infected by Neospora caninum from Bovine

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