贵州省猪源大肠杆菌对四环素类药物的耐药性及耐药基因检测

doi:10.16431/j.cnki.1671-7236.2018.05.030

Abstract

Abstract:

In order to investigate the resistance to tetracycline and the prevalence of resistant genes of E. coli in Guizhou province,the sensitivity of 783 E. coli isolated from swine of 8 farms in Guizhou province to 6 kinds of tetracyclines was determined by broth microdilution method,and tetracycline resistance gene was detected by PCR method.The results showed that the resistance rates of isolates to oxytetracycline,tetracycline,chlortetracycline,doxycycline,minocycline and tigecycline were 97.3%,97.6%,96.6%,89.3%,48.0% and 34.2%,respectively.The resistance levels of oxytetracycline and tetracycline were the highest,their MIC₅₀ were 256 and 128 μg/mL,respectively,while the MIC₉₀ were both 512 μg/mL,and the drug resistance times of tetracycline were higher than that of oxytetracycline.The drug resistance level of minocycline and tigecycline were the lowest,and their MIC₉₀ were 32 and 4 μg/mL,respectively,the drug resistance times of minocycline and tigecycline were the same.The detection rates of tetracycline resistance genes tetA,tetB,tetC and tetD were 92.60%,41.50%,58.75%,58.62% and 70.10%,respectively.The tetA gene was mainly type in resistance strains,and most of them carried complex resistance genes.The resistance rates of E.coli to oxytetracycline,tetracycline,chlortetracycline,doxycycline and minocycline were extremely significantly correlated with the detection rates of tetB and tetM genes (P<0.01).The detection of tetD gene was positively with tigecycline and minocycline resistance (P<0.01) respectively.The drug resistance of E. coli from swine in Guizhou province was widespread,especially to tetracycline and oxytetracycline;The drug resistance trend of chlortetracycline and doxycycline were highly;The mechanism of efflux pump was the main drug resistance mechanism of tetracyclines;It had a great relationship between the prevalence of resistance genes and growth of antimicrobial resistance in veterinary clinical practice.

Key words: drug sensitivity test; tetracyclines; drug resistance genes; drug-resistant phenotype

CLC Number:

S852.61

WANG Xuejun, TAN Aijuan, LV Shiming, DU Anding, CHEN Boli, ZHANG Shengxun. Detection of Resistance and Resistance Genes of Swine Escherichia coli in Guizhou to Tetracyclines[J]. , 2018, 45(5): 1367-1373.

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Detection of Resistance and Resistance Genes of Swine Escherichia coli in Guizhou to Tetracyclines

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