常用中草药对水产动物致病菌体外抑菌效果研究

doi:10.16431/j.cnki.1671-7236.2018.05.029

Abstract

Abstract:

The experiment was aimed to study antibacterial effect of common Chinese herbs such as Phellodendron amurense,Terminalia chebula,Rhizoma anemarrhenae,Caesalpinia sappan etc.on aquatic animal pathogenic bacteria in vitro and provide reference for the prevention and control of bacterial diseases in aquaculture.In this experiment,Photobacterium damsela,Aeromonas hydrophila,Vibrio harveyi and Vibrio vulnificus isolates were the test strains,the diameter of inhibition zone,minimum inhibitory concentration (MIC),minimum bactericidal concentration (MBC) and fractional inhibitory concentration index (FICI) were detected to study the antibacterial activity of Chinese herbal medicine on common aquatic pathogenic bacteria.Effective ingredients of Chinese herbal medicine was extracted by water,the inhibitory effect of Chinese herbal medicine on aquatic pathogenic bacteria was determined by agar plate punching method,the MIC and MBC of Chinese herbal medicine were determined by microbroth dilution method,the FICI was determined by broth microdilution method.The results showed that 16 kinds of Chinese herbal medicine had different degrees of bacteriostasis to aquatic pathogenic bacteria.Drug sensitivity test showed that Caesalpinia sappan,Ligustrum lucidum,Prunella vulgaris and Pericarpium citri reticulatae viride had bacteriostatic effect on 4 kinds of aquatic pathogenic bacteria,the inhibition zone diameter were from 10.5 to 29.0 mm,and all 4 pathogenic bacteria were resistant to Portulaca oleracea;The MIC test results showed that the MIC of Caesalpinia sappan,Ligustrum lucidum,Prunella vulgaris and Pericarpium citri reticulatae viride on 4 kinds of pathogenic bacteria were from 7.81 to 250.00 mg/mL;The MBC of Caesalpinia sappan,Ligustrum lucidum,Prunella vulgaris and Pericarpium citri reticulatae viride on 4 kinds of pathogenic bacteria were from 7.81 to 500.00 mg/mL.Combined antimicrobial test results showed that with Caesalpinia sappan,Prunella vulgaris,Ligustrum lucidum and Pericarpium citri reticulatae viride combined with only Photobacterium damsela played a synergistic inhibitory effect,no inhibitory effect on Aeromonas hydrophila,Vibrio vulnificus and Vibrio harveyi.The combination of Ligustrum lucidum and Pericarpium citri reticulatae viride had an additive effect on the bacteriostatic action of Vibrio harveyi only,the combination of other pathogenic bacteria in the inhibitory effect showed independent or antagonistic effects,other drug combinations in the 4 kinds of pathogenic bacteria antibacterial effect on performance of unrelated effect or antagonistic effect.The results of the combined inhibition zone showed that the combination of Chinese herbal medicine had different bacteriostatic effects on the four pathogens.The results showed that common Chinese herbal medicine had a certain inhibitory effect on the aquatic pathogens.

Key words: Chinese herbal medicine; in vitro antibacterial effect; microbroth dilution method; minimum inhibitory concentration (MIC); minimum bactericidal concentration (MBC)

CLC Number:

S853.72

WANG Hongbin, ZHU Lixia, YANG Nan, WU Tonglei, ZHANG Zhiqiang, GAO Guisheng, SHI Qiumei. Study on Antibacterial Effects of Common Chinese Herbs Against Aquatic Animal Pathogens in vitro[J]. , 2018, 45(5): 1357-1366.

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Study on Antibacterial Effects of Common Chinese Herbs Against Aquatic Animal Pathogens in vitro

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