中草药复方防治肉鸡骨病的研究

doi:10.16431/j.cnki.1671-7236.2021.01.042

Abstract

Abstract: In order to prove the prevention and treatment effect of Chinese herbal medicine compound on broiler bone disease,this experiment selected 160 1-day-old healthy Kosbao broilers and randomly divided into 8 groups to be raised to 30 and 60 days old,a compound of Chinese herbal medicine complex consistion of 8 Chinese herbal medicine such as Angelica sinensis,Fructus Lycii and Dioscorea oppositifolia L..In the experiment,high (0.6%),medium (0.4%),and low (0.2%) dose of Chinese herbal medicine were used,and control group was fed a basic diet.At the age of 30 and 60 days,weight were detected.Biochemical analyzer was used to detect the blood biochemical indexes bone metabolism related (VDR,OT,OPG,ALP),the bone strength (elastic modulus,yield strength and maximum stress) of the tibia was detected by the universal test machine three point bending test,and the Real-time PCR method was used to detect the difference of mRNA expression of OPG,Runx-2,RANK,RANKL,TNF-α and BMP-2 genes in the thoracic and leg cartilage tissue.The results showed that the Chinese herbal medicine compound could gain weight,increase the content of VDR,OT,OPG and ALP in serum (P<0.05) at 60 days old,30 days old was higher than that of 60 days old.It could enhance tibial bone strength (P<0.05),30 days old was higher than that of 60 days old.And it could also promote the expression of OPG,BMP-2 and Runx-2 genes and inhibit the expression of TNF-α,RANK and RANKL genes (P<0.05).From above mentioned results,considering the relative indexes of live weight and bone strength,compound Chinese herbal medicine high dose group was the best formula to improve the bone characteristics of the broilers.The compound Chinese herbal medicine could improve the content of VDR,OT,OPG and ALP in serum,regulate the OPG/RANK/RANKL system to maintain the dynamic balance of bone resorption and bone formation,promote the expression of BMP-2 and Runx-2 genes in the important pathway of bone formation BMP-2/Smads/Runx-2,and inhibit TNF-α gene expression,so as to improve the skeleton characteristics of Kosbao broilers,and effectively prevent bone disease of broilers.

Key words: Chinese herbal medicine; Kosbao broiler; bone disease; related gene

CLC Number:

S853.74

DUAN Xiaohua, XU Zhiqiang, GE Changrong, JIA Junjing, DOU Tengfei. Prevention and Treatment of a Chinese Herbal Medicine Compound for Bone Disease in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 385-394.

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Prevention and Treatment of a Chinese Herbal Medicine Compound for Bone Disease in Chickens

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