藏鸡和隐性白羽鸡感染柔嫩艾美耳球虫后免疫相关基因的表达变化分析

doi:10.16431/j.cnki.1671-7236.2021.04.032

Abstract

Abstract: In order to preliminarily explore the molecular mechanism of the difference in the susceptibility of Tibetan chickens and Recessive White chickens to Eimeria tenella (E. tenella) from the perspective of immunogenetics,1×10⁵ live E. tenella sporulated oocysts were used to infect Tibetan chickens and Recessive White chickens,respectively,which displayed different levels of susceptibility to E.tenella infection.The transcription level of IFN-γ,IL-2,IL-16,TLR3 and TLR15 in spleen,cecum,thymus and bursa of Fabricius were detected by Real-time PCR at 0 d before infection and at the 2nd,4th,6th and 8th day after infection.The results showed that the transcription levels of IFN-γ,IL-2,IL-16,TLR3 and TLR15 in the spleen of Tibetan chickens were up-regulated on the 4th and 8th day after infection,while that of Recessive White chickens were not obviously changed.The transcription level of IFN-γ in the cecum of Tibetan chickens were significantly up-regulated on the 2nd day after infection (P<0.05),TLR3 was significantly up-regulated from the 4th day after infection (P<0.05),and other immune-related genes showed little change.The transcription level of IFN-γ in the cecum of Recessive White chickens were significantly up-regulated on the 8th day after infection (P<0.05),IL-2 was significantly up-regulated from the 2nd day after infection (P<0.05),IL-16 was significantly up-regulated from the 6th day after infection (P<0.05),TLR3 was significantly up-regulated on the 2nd and 8th day after infection (P<0.05),and TLR15 had little change.All immune related genes were up-regulated or down-regulated in thymus and bursa of Fabricius of the two breeds,but the transcription level of TLR3 and TLR15 in bursa of Fabricius of Tibetan chickens showed relatively large changes,while the other immune-related genes showed little changes compared with that before infection.The above results showed that E. tenella infection mainly resulted in significant changes in immune related genes in spleen and cecum of Tibetan chickens and Recessive White chickens,indicating that the genetic background of host would affect the immune response of coccidiosis infection to a certain extent.

Key words: Tibetan chickens; Recessive White chickens; Eimeria tenella; immune-related genes; Real-time PCR

CLC Number:

S852.72⁺3

LI Jianmei, JI Zhengjian, LIU Mei, SHEN Xinyue, CHENG Xu, YU Yan, DAI Yabin. Expression Changes of Immune-related Genes in Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1440-1448.

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Expression Changes of Immune-related Genes in Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection

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