China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (1): 90-97.doi: 10.16431/j.cnki.1671-7236.2020.01.011

• Genetics and Breeding • Previous Articles     Next Articles

Expression and Bioinformatics Analysis of Mastitis Resistance Related Genes in Xinjiang Brown Cattle

JIANG Hui1, LIU Pan1, LI Yuanyuan1, LIU Kuiguo2, HUANG Xixia1, LIU Jiangwei2, XU Lei1, DU Jianwen1, SANG Zhagen1, YANG Xuemei1, ZHAO Fanfan1, WANG Dan1   

  1. 1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
    2. Xinjiang Brown Cattle Breeding Center, Cattle Farm in Urumqi, Urumqi 830049, China
  • Received:2019-08-12 Online:2020-01-20 Published:2020-01-17

Abstract: The aim of this study was to analyze the molecular biological functions of mastitis resistance related genes in Xinjiang Brown cattle,and lay a theoretical foundation for further development of mastitis resistance traits in Xinjiang Brown cattle by molecular marker-assisted breeding.Functional GO enrichment analysis and KEGG signaling pathway analysis were performed by DAVID 6.8 software for the genes related to mastitis resistance reported (20 genes in total) in previous studies and related literature.Using String 10.0 database for protein interaction network analysis,combined with bioinformatics analysis results and related literature research results,JAK2 and STAT5A genes were selected.Real-time PCR was used to detect the expression of JAK2 and STAT5A genes in blood of Xinjiang Brown cattle with different breast health.GO enrichment analysis showed that 20 genes mainly involved biological processes such as cell membrane and immune response regulation defense reaction;KEGG analysis found that genes were mainly involved in JAK-STAT signaling pathway,chemokine signaling pathway,ErbB signaling pathway,etc;PPI analysis showed that there were direct or indirect links between 8 genes in the 20 genes.Fluorescence quantitative test showed that the expression of JAK2 and STAT5A genes in blood of healthy Xinjiang Brown cattle was extremely significantly or significantly higher than that of mastitis Xinjiang Brown cattle(P<0.01;P<0.05).The JAK2 and STAT5A genes were screened by bioinformatics analysis.Furthermore,the intrinsic relationship between Xinjiang Brown cattle with different breast health and JAK2 and STAT5A gene expression was discussed at the molecular level.

Key words: JAK2 gene; STAT5A gene; Real-time PCR; mastitis resistance; Xinjiang Brown cattle

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