香猪卵巢StAR和CYP11A1基因的差异表达研究

doi:10.16431/j.cnki.1671-7236.2018.05.002

Abstract

Abstract:

In order to reveal the molecular mechanism of Xiang pig reproduction, StAR and CYP11A1 genes of ovary in Xiang pigs were analyzed.Chosing two groups of Xiang pig with high and low litter sizes as samples,the expression level of two genes were further detected from ovary by Real-time PCR method.And the promoter regions of two genes were cloned and sequenced from the genomic DNA.The results showed that both of FPKM values from the RNA-seq for transcriptomes and the mRNA levels by Real-time PCR in the group with high litter size were much higher than the other one.In the promoter region of StAR gene with 1 144 bp,the binding elements of a total of 72 transcription factors (TF,34 kinds) were deduced,including ER,COUP and Sp1,etc.About 8 SNP sites were determined from sequence of 68 to 698 bp upstream of the transcription initiation site.All of SNP sites were outside of the binding sites for TFs.For the promoter of CYP11A1 gene in 970 bp,there presented 75 TFs (30 kinds),such as Sp1,PU.1 and C/EBPdel.About 5 SNP sites were detected in the region of 12 to 593 bp upstream of the start site,in which a polymorphism site of C-T at 69 bp would change the binding of SP1.The frequency of T allele was 17% and 33% in Xiang pigs with high or low litter sizes,respectively.The SNP site at 69 bp in the promoter region might be a reason for the different expression of CYP11A1 gene in pig ovary.

Key words: Xiang pig; ovary; Real-time PCR; StAR gene; CYP11A1 gene; promoter

CLC Number:

S828

MAO Ning, WANG Jiafu, ZHANG Fuping, TANG Liangting, RUAN Yiqi, YI Fanli, RAN Xueqin. Study on Differential Expression of StAR and CYP11A1 Genes in Xiang Pig Ovary[J]. , 2018, 45(5): 1137-1144.

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Study on Differential Expression of StAR and CYP11A1 Genes in Xiang Pig Ovary

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