树鼩Tssk6基因cDNA克隆、组织表达谱及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2017.03.001

Abstract

Abstract:

The purpose of this study was to clone the full-length cDNA of tree shrew's Tssk6 gene, to elucidate its tissue expression profiles and fundamental bioinformatical characteristics. In the present study, the full-length cDNA of tree shrew's Tssk6 gene was cloned with specific primers on the basis of predicted mRNA sequence of tree shrew's Tssk6 gene in GenBank, the mRNA expression of Tssk6 gene in various tree shrew's tissues was investigated via Real-time PCR. In addition, the bioinformatics on nucleotide sequence of CDS region of Tssk6 cDNA in tree shrew as well as putative protein structure had been analyzed. The results showed that:The CDS region of tree shrew's Tssk6 cDNA contained 822 bp encoding 273 amino acids; Tssk6 gene specifically expressed in tree shrew's testis; Based on the molecular phylogenetic tree constructed with CDS of Tssk6 cDNA among tree shrew and other eight mammalian species, it was inferred that tree shrew was genetically close to primates like human, chimpanzee and rhesus monkey, while it was genetically far to rodents like mouse, rat and golden hamster. This study would lay a foundation for the further study of Tssk6 gene function in tree shrew.

Key words: tree shrew; Tssk6 gene; cDNA; cloning; bioinformatics; Real-time PCR

CLC Number:

R-332

LI Yuan-ji, YANG Ming-hua, LIU Fang, ZHU Fang-fang, HE Shao-yu, KUANG Hai-ou, LI Ya-hui. cDNA Cloning, Tissue Expression Profiles and Bioinformatical Analysis of Tssk6 Gene in Tree Shrews[J]. , 2017, 44(3): 619-627.

References

[1] 沈培清, 郑红, 刘汝文, 等. 中国树鼩实验动物化研究进展和展望[J]. 动物学研究, 2011, 32(1):109-114.
[2] Fan Y, Huang Z Y, Cao C C, et al. Genome of the Chinese tree shrew[J]. Nat Commun, 2013, 4(1426):66-78.
[3] Wang J, Xu X, Ding Z, et al. Basal physiological parameters in domesticated tree shrews (Tupaia belangeri chinensis)[J]. Zoological Research, 2013, 34(E2):E69-E74.
[4] 李贵, 严晔, 常云艳, 等. 树鼩实验室繁殖的新方法[J]. 现代畜牧兽医,2009, 3:18-21.
[5] 殷安国, 匡德宣, 李晓飞, 等. 树鼩模型在人类病毒性疾病研究中的应用进展[J]. 中国实验动物学报, 2014, 22(2):86-89.
[6] 杨香娣, 曹骥, 杨春, 等. 荧光标记微卫星分析广西树鼩群体的遗传多态性[J]. 中国畜牧兽医, 2016, 43(1):182-190.
[7] 徐林, 张云, 梁斌, 等. 实验动物树鼩和人类疾病的树鼩模型研究概述[J]. 动物学研究, 2013, 34(2):59-69.
[8] Ping S, Yue F, Wang C, et al. Effects of dimethyl sulfoxide, ethylene glycol, propylene glycol, and glycerol on cryopreservation of wild tree shrew (Tupaia belangeri Chinese) cauda epididymal sperm[J]. Zoological Research, 2012, 11(19):3568-3574.
[9] Ping S H, Wang C Y, Tang W R, et al. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)[J]. Zoological Research, 2012, 33(1):19-28.
[10] 黄实,苏金珠,李亚辉. 睾丸特异性丝/苏氨酸蛋白激酶家族研究进展[J]. 云南农业大学学报(自然科学),2012, 27(3):424-429.
[11] Hao Z, Jha K N, Kim Y H, et al. Expression analysis of the human testis-specific serine/threonine kinase (TSSK) homologues. A TSSK member is present in the equatorial segment of human sperm[J]. Molecular Human Reproduction, 2004,10(6):433-444.
[12] Spiridonov N A, Wong L, Zerfas P M, et al. Identification and characterization of SSTK, a serine/threonine protein kinase essential for male fertility[J]. Molecular and Cellular Biology, 2005,25(10):4250-4261.
[13] Li Y H, Sosnik J, Brassard L, et al. Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis[J]. Molecular Human Reproduction, 2010, 17(1):42-56.
[14] 吴昊天,梁磊. 人类Izumo4和Tssk6在精子顶体反应前后的定位检测[J]. 中国现代医学杂志,2016, 26(17):45-48.
[15] 苏丹,张思仲. TSSK6基因的突变筛查与男性不育的相关研究[A]. 中华医学会医学遗传学分会、中国遗传学会人类和医学遗传学委员会.第八次全国医学遗传学学术会议(中华医学会2009年医学遗传学年会)论文摘要汇编[C].中华医学会医学遗传学分会、中国遗传学会人类和医学遗传学委员会,2009.
[16] Sosnik J, Miranda P V, Spiridonov N A, et al. Tssk6 is required for Izumo relocalization and gamete fusion in the mouse[J]. Journal of Cell Science, 2009, 122(15):2741-2749.
[17] 徐亦力. 内含子类型与捻接机制[J]. 自然杂志,1988, 11(8):574-585.
[18] Poonkhum R, Pongmayteegul S, Meeratana W, et al. Cerebral microvascular architecture in the common tree shrew (Tupaia glis) revealed by plastic corrosion casts[J]. Microscopy Research and Technique, 2000, 50(5):411-418.
[19] Wang P, Huo H L, Wang S Y, et al. Cloning, sequence characterization, and expression patterns of members of the porcine TSSK family[J]. Genet Mol Res, 2015, 14(4):14908-14919.
[20] Bustin S A, Benes V, Garson J A, et al. The MIQE guidelines:Minimum information for publication of quantitative Real-time PCR experiments[J]. Clin Chem, 2009, 55(4):611-622.
[21] Schmittgen T D, Livak K J. Analyzing Real-time PCR data by the comparative CT method[J]. Nature Protocols, 2008, 3(6):1101-1108.
[22] Xu B, Hao Z, Jha K, et al. Validation of a testis specific serine/threonine kinase[TSSK] family and the substrate of TSSK1 & 2, TSKS, as contraceptive targets[J]. Society of Reproduction and Fertility Supplement, 2006, 63:87-101.

cDNA Cloning, Tissue Expression Profiles and Bioinformatical Analysis of Tssk6 Gene in Tree Shrews

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[3]	LI Yongbin, YANG Huilin, SONG Zihao, ZHANG Yu, YANG Jin, MENG Jia, CUI Xiaozhen, LYU Xiaoling, ZHENG Mingxue, BAI Rui. Cloning and Bioinformatics Analysis of MIF Gene in Eimeria tenella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2450-2459.
[4]	ZHANG Dianqi, MA Xinhao, YANG Zhimei, MEI Chugang, ZAN Linsen. Bioinformatics Analysis of PAI1 Gene and Its Effect on the Proliferation and Differentiation of Intramuscular Adipocytes in Qinchuan Beef Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1729-1741.
[5]	WANG Haorui, YU Baojun, YANG Jiayi, WEI Rong, FU Xi, WANG Chuanchuan, ZHANG Di, LI Desheng, CAI Zhengyun, GU Yaling, ZHANG Juan. Bioinformatics Analysis and Tissue Expression of DERA Gene in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1764-1773.
[6]	CHEN Meiting, ZHENG Congsen, LIANG Zexian, LIN Qiaoer, CHANG Chuanzhe, ZHOU Jun, FENG Jun, LI Linlin, QIN Limei. Cloning and Bioinformatics Analysis of env Gene of Avian Leukosis Virus Subgroup J Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1785-1795.
[7]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[8]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[9]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[10]	TAO Qinghua, HUANG Caiyun, CHEN Chao, LIU Wei, SUN Ningning, LI Ang. Cloning and Tissue Expression Difference Analysis of LIF Gene in White Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1296-1306.
[11]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[12]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[13]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[14]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[15]	SONG Xingchao, MENG Jinzhu, ZHAO Yuanyuan, WU Zhenyang, AN Qingming. Cloning and Bioinformatics Analysis of MyoG Gene Promoter Region Sequence in Guizhou White Goat [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 893-903.