柔嫩艾美耳球虫对藏鸡和隐性白羽鸡致病性差异分析

doi:10.16431/j.cnki.1671-7236.2019.10.028

Abstract

Abstract: In order to further clarify the pathogenicity of Eimeria tenella in chickens with different susceptibility to coccidia,we infected Tibetan chickens and Recessive White chickens with resistance to coccidia at the dosage of 1×10⁵ sporulated oocysts of Eimeria tenella.After inoculation,the clinical characteristics,blood stool score,mortality and susceptibility to coccidia were observed and recorded.Weight gain,cecum lesion score and oocyst yield were recorded.Five chickens were randomly selected from each breed before infection and 3,6 and 8 days after infection to collect anticoagulant.The number of CD4⁺,CD8⁺ T lymphocyte subsets in peripheral blood was detected by flow cytometry (FCAS).The results showed that the relative weight gain rate of Tibetan chickens infected with Eimeria tenella was higher than that of Recessive White chickens.The mortality rate,blood stool score and cecum lesion score of Tibetan chickens were lower than those of Recessive White chickens,but the oocyst yield was higher than that of Recessive White chickens.Before infection,the number of CD4⁺ T lymphocyte and the ratio of CD4⁺/CD8⁺ in Tibetan chickens were higher than those in Recessive White chickens.On the 3rd day after infection,the number of CD4⁺,CD8⁺ T lymphocyte and CD4⁺/CD8⁺ ratio in Tibetan chickens decreased,while the number of CD8⁺ T lymphocyte in Recessive White chickens decreased slightly,the number of CD4⁺ T lymphocyte and the ratio of CD4⁺/CD8⁺ increased,but the ratio of CD4⁺/CD8⁺ was still significantly lower than that in Tibetan chickens (P<0.05).On the 6th day after infection,the number of CD4⁺ T lymphocyte and the ratio of CD4⁺/CD8⁺ decreased in both breeds,Tibetan chickens showed a significant decrease (P<0.05),while only the ratio of CD4⁺/CD8⁺ in Recessive White chickens significantly decreased (P<0.05),the number of CD8⁺ T lymphocyte in Recessine White chickens significantly increased (P<0.05).On the 8th day after infection,the ratio of CD4⁺/CD8⁺ in two breeds of chickens significantly decreased (P<0.05).The number of CD8⁺ T lymphocyte in Tibetan chickens was significantly higher than that in Recessive White chickens (P<0.05),but the ratio of CD4⁺/CD8⁺ was significantly lower than that in Recessive White chickens (P<0.05).The results indicated that the pathogenicity of coccidia to Tibetan chickens and Recessive White chickens was different,which was closely related to the immune response mediated by T lymphocyte.

Key words: Tibetan chickens; Recessive White chickens; Eimeria tenella; pathogenicity; T lymphocyte subpopulations

CLC Number:

S852.72

LI Jianmei, LIU Mei, SHEN Xinyue, DAI Yabin, YU Yan. Analysis of Pathogenicity Difference Between Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3058-3064.

References

[1] 冯玉萍,韩天飞,吕强华,等.L-NMMA对鸡球虫早熟弱毒疫苗免疫鸡增重的影响[J].中国畜牧兽医,2019,46(3):865-872. FENG Y P,HAN T F,LYU Q H,et al.Effect of L-NMMA on weight gain of chickens infected with chicken coccidian premature attenuated vaccine[J].China Animal Husbandry & Veterinary Medicine,2019,46(3):865-872.(in Chinese)
[2] LI C,YAN X,LILLEHOJ H S,et al.Eimeria maxima induced transcriptional changes in the cecal mucosa of broiler chickens[J].Parasites & Vectors,2019,12(1):285-293.
[3] BLAKE D P,TOMLEY F M.Securing poultry production from the ever-present Eimeria challenge[J].Trends in Parasitology,2014,30(1):12-19.
[4] CLARKE L,FODEY T L,CROOKS S R,et al.A review of coccidiostats and the analysis of their residues in meat and other food[J].Meat Science,2014,97(3):358-374.
[5] LOOI F Y,BAKER M L,TOWNSON T,et al.Creating disease resistant chickens:A viable solution to avian influenza[J].Viruses,2018,10(10):561-569.
[6] 张娇.地方鸡对马立克氏病的抗性研究及FSHβ基因的遗传变异[D].泰安:山东农业大学,2013. ZHANG J.Study on resistance of local chicken to Marek’s disease and FSHβ genetic variation[D].Tai’an:Shandong Agricultural University,2013.(in Chinese)
[7] 周秀红.麻黄、红宝及其杂交鸡对大肠杆菌病和新城疫的遗传抗性研究[D].合肥:安徽农业大学,2003. ZHOU X H.Genetic resistance of spotted-brown,redbro chicken & their reciprocal hybrids to collibacilloisis and Newcastle disease[D].Hefei:Anhui Agricultural University,2003.(in Chinese)
[8] MSOFFE P L,MINGA U M,MTAMBO M M,et al.Differences in resistance to Salmonella enterica serovar Gallinarum infection among indigenous local chicken ecotypes in Tanzania[J].Avian pathology,2006,35(4):270-276.
[9] 甘建伉,张泽樘,罗庆斌.鸡沙门氏菌抗性基因研究进展[J].中国家禽,2012,34(11):48-50. GAN J K,ZHANG Z T,LUO Q B.Research progress on resistance genes of Salmonella in chickens[J].China Poultry,2012,34(11):48-50.(in Chinese)
[10] 甘师仪,王劼,罗成龙,等.A亚型禽白血病的遗传抗性位点与肉鸡重要经济性状的关联分析[J].畜牧兽医学报,2019,50(4):721-726. GAN S Y,WANG J,LUO C L,et al.Association of genetic resistance loci to subgroup A avian leucosis virus with important economic traits in broilers[J].Acta Veterinaria et Zootechnica Sinica,2019,50(4):721-726.(in Chinese)
[11] 晁哲,吴望军,邢漫萍,等.鸡HSP90AA1基因SNP检测及启动子区CpG岛的甲基化研究[J].中国畜牧兽医,2019,46(2):504-511. CHAO Z,WU W J,XING M P,et al.Detection of single nucleotide polymorphism and methylation of CpG island in promoter region of chicken HSP90AA1 gene[J].China Animal Husbandry & Veterinary Medicine,2019,46(2):504-511.(in Chinese)
[12] KIM E S,HONG Y H,MIN W,et al.Fine-mapping of coccidia-resistant quantitative trait loci in chickens[J].Poultry Science,2006,85(11):2028-2030.
[13] HONG Y H,KIM E S,LILLEHOJ H S,et al.Association of resistance to avian coccidiosis with single nucleotide polymorphisms in the zyxin gene[J].Poultry Science,2009,88(3):511-518.
[14] KIM E S,HONG Y H,LILLEHOJ H S.Genetic effects analysis of myeloid leukemia factor 2 and T cell receptor-beta on resistance to coccidiosis in chickens[J].Poultry Science,2010,89(1):20-27.
[15] 韩新畴.文昌鸡、AA鸡、海兰鸡和三黄鸡对柔嫩艾美耳球虫易感性研究[J].中兽医医药杂志,2007,41(1):39-40. HAN X C.Study on susceptibility of Wenchang,AA,Hyline and three buff chickens to Eimeria tenella infection[J].Journal of Traditional Chinese Veterinary Medicine,2007,41(1):39-40.(in Chinese)
[16] 窦新红,童海兵,许明,等.不同品种地方鸡对柔嫩艾美球虫的易感性分析[J].畜牧与兽医,2009,41(1):7-9. DOU X H,TONG H B,XU M,et al.Susceptibilities of four local breeds of chickens to Eimeria tenella infection[J].Animal Husbandry & Veterinary Medicine,2009,41(12):7-9.(in Chinese)
[17] 李建梅,刘梅,沈欣悦,等.我国不同地方品种鸡(Gallus gallus domesticus)对柔嫩艾美耳球虫的易感性[J].畜牧兽医学报,2016,47(10):2098-2107. LI J M,LIU M,SHEN X Y,et al.The susceptibility of Chinese indigenous chicken breeds (Gallus gallus domesticus) to Eimeria tenella infection[J].Acta Veterinaria et Zootechnica Sinica,2016,47(10):2098-2107.(in Chinese)
[18] 索勋,李国清.鸡球虫病学[M].北京:中国农业大学出版社,1998. SUO X,LI G Q.Coccidia and Coccidiosis of Domestic Fowl[M].Beijing:China Agricultural University Press,1998.(in Chinese)
[19] JOHNSON J,REID W M.Anticoccidial drugs:Lesion scoring techniques in battery and floor-pen experiments with chickens[J].Experimental Parasitology,1970,28(1):30-36.
[20] SWAGGERTY C L,GENOVESE K J,HE H,et al.Broiler breeders with an efficient innate immune response are more resistant to Eimeria tenella[J].Poultry Science,2011,90(5):1014-1019.
[21] LEES H,DONG X,LILLEHOJ H S,et al.Comparing the immune responses of two genetically B-complex disparate Fayoumi chicken lines to Eimeria tenella[J].British Poultry Science,2016,57(2):165-171.
[22] 胡京友,刘晶,郑世民.鸡球虫病免疫学研究进展[J].动物医学进展,2004,25(3):25-27. HU J Y,LIU J,ZHENG S M.Advances in immunology of chicken coccidiosis[J].Progress in Veterinary Medicine,2004,25(3):25-27.(in Chinese)
[23] SWINKELS W J,POST J,CORNELISSEN J B,et al.Immune responses to an Eimeria acervulina infection in different broilers lines[J].Veterinary Immunology and Immunopathology,2007,117(1-2):26-34.

Analysis of Pathogenicity Difference Between Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection

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