阳离子脂质体包埋角鲨烯佐剂的制备及理化性质研究

doi:10.16431/j.cnki.1671-7236.2021.04.031

Abstract

Abstract: To study the preparation process of a cationic liposome-embedded squalene adjuvant,and the lecithin,cholesterol and steary lamine were used as raw materials to coat metabolizable lipid squalene with immune function,using the method of ethanol injection combined with probe ultrasound in this experiment.Furthermore,the particle size,stability index (TSI) and Zeta potential were used as evaluation indexes and the physical and chemical properties of cationic liposome-coated squalene adjuvant were analyzed.The results showed that the optimal preparation conditions of cationic liposome-coated squalene adjuvant were lecithin 15 mg/mL,cholesterol 2.0 mg/mL,squalene 8 μL/mL,probe ultrasonic time 15 min and ultrasonic power 100 W.Under the optimum conditions,the adjuvant could be obtained with an average potential of 45 mV,an average particle size of 182 nm,and a viscosity of less than 80 mPa·s,meanwhile,it also had a great significance in further researching as a vaccine adjuvant with small particle size,low viscosity and stable performance.

Key words: cationic liposome; squalene; adjuvant; viscosity

CLC Number:

S816.8

WAN Meng, CHEN Yilin, QIAN Yuhao, HUANG Xuan, XIA Lianchen, WANG Chunwei, ZHU Aixia. Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1431-1439.

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Preparation of Cationic Squalene Liposome Adjuvant and Physical and Chemical Properties

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