›› 2015, Vol. 42 ›› Issue (9): 2467-2473.doi: 10.16431/j.cnki.1671-7236.2015.09.037

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Preparation of Adjuvant Diluent for Live Vaccine against Classical Swine Fever and Evaluation of its Immune Effects

LIANG Kong-xian1, LV Xiao-jun2, DING Dan1, XU Qi3, ZHOU Jie2, LIU Wei1,4   

  1. 1. College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;
    2. Hubei Institute for Food and Drug Control, Wuhan 430074, China;
    3. Wuhan Best-carrier Nano Science and Technology Co., Ltd., Wuhan 430074, China;
    4. National Engineering Research Center for Nanomedicine, Huazhong University of Science and Technology, Wuhan 430074, China
  • Received:2015-03-16 Online:2015-09-20 Published:2015-09-25

Abstract: The aim of this study was to develop a novel oil-in-water (O/W) emulsion as adjuvant diluents (AD) for live vaccine against classical swine fever (CSF) that could effectively enhance the immune effect of vaccine.The AD was prepared by high-pressure homogenization technique.Formulations and preparation parameters were optimized with response surface design.Its stability, particle size, polydispersity (PDI) and Zeta potential were characterized.The humoral immune response and cellular immune response of the AD were evaluated with BALB/c mice by intramuscular injection.The particle size of the AD prepared by optimized formulation and parameters was 100.4 nm, PDI was 0.147, and Zeta potential was —28.7 mV.The experiment results showed that the AD had good stability.The AD was inoculated combined with live vaccine against CSF into BALB/c mice by intramuscular injection.The results showed that the live vaccine against CSF specific immune responses could be evoked in mice by co-inoculation with AD and vaccine.The cellular immune response levels in co-inoculated groups were significantly higher than control group (P<0.05), with obvious phenomena of higher levels of IFN-γ, IL-6 and IL-4 in serum.The result revealed that cellular immune capability significantly improved with the AD.The results strongly revealed that cellular immune capability significantly improved by introducing AD for effective immune-adjuvant for live vaccine against CSF.

Key words: live vaccine against classical swine fever; adjuvant diluents (AD); high-pressure homogenization technique; response surface design

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