大黄体外抗猪流行性腹泻病毒的研究

doi:10.16431/j.cnki.1671-7236.2015.09.038

Abstract

Abstract: The inhibitory effects of rhubarb on cell lesion, which was caused by porcine epidemic diarrhea virus (PEDV), and its mechanism had been studied in this research.The maximum safe concentration of rhubarb solution applied to Vero cells was determined through morphological observation, and then the PEDV infected Vero cell model in vitro was established, by which the inhibitory effect of rhubarb on virus was studied.The experimental method had also been established based on this cell model.The result showed that the maximum safe concentration was 3.28 mg/mL.Both morphological observation and MTT assay were indicated that rhubarb could not only block the attachment of the virus to Vero cell and inhibit the synthesis and reproduction of the virus, but also could directly inactivate the virus.This research showed that rhubarb had an inhibitory effect on PEDV, and provided a theoretical basis for the prevention and treatment of clinical porcine epidemic diarrhea.

Key words: rhubarb; anti-viral in vitro; porcine epidemic diarrhea virus (PEDV)

CLC Number:

S852.65

CHEN Tian-tian, CHENG Fu-liang, ZHANG Jian-mei, NIE Zhao-jing, FANG Dong, FAN Mei-na, LI Fu-hui, CHENG He-gang. Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro[J]. , 2015, 42(9): 2474-2480.

References

[1] Chen J F,Sun D B,Wang C B,et al.Molecular characterization and phylogenetic analysis of membrane protein genes of porcine epidemic diarrhea virus isolates in China[J].Virus Genes,2008,36:355-364.

[2] 刘孝珍.2011年中国猪流行性腹泻病毒分子流行病学初步调查[D].哈尔滨:东北农业大学,2012.

[3] Lin C N,Chung W B,Chang S W,et al.US-like strain of porcine epidemic diarrhea virus outbreaks in Taiwan,2013-2014[J].J Vet Med Sci,2014,76(9):1297-1299.

[4] 焦茂兴,吴峰,刘德辉,等.猪流行性腹泻病毒重组腺病毒疫苗的构建及小鼠免疫试验[J].中国畜牧兽医,2012,39(2):1-16.

[5] 陈申秒,牛成明,何福庆,等.猪流行性腹泻病毒研究进展及疫苗应用前景[J].中国畜牧兽医,2014,41(3):223-229.

[6] Naemi F,Asghari G,Yousofi H,et al.Chemical composition of essential oil and anti trichomonas activity of leaf,stem,and flower of Rheum ribes L.extracts[J].Avicenna J Phytomed,2014,4(3):191-199.

[7] 徐庆,谭永俊,苏小建,等.掌叶大黄化学成分研究[J].中草药,2009,40(4):533-536.

[8] 王芃,解砚英,王元书,等.中药大黄抗病毒作用的实验研究[J].山东医科大学学报,1996,34(2):166-169.

[9] 梁荣感,罗伟生,李利亚,等.大黄蒽醌类化合物体外抗流感病毒作用的研究[J].华夏医学,2006,19(3):396-398.

[10] 宋艳艳,王志玉,钟蒙,等.不同药物抗风疹病毒的实验研究[J].山东医科大学学报,2000,38(2):151-155.

[11] 刘妮,朱辫弦,黄正昌,等.大黄醇提液对2.2.15细胞分泌HBsAg、HBeAg的抑制作用[J].中药材,2004,27(6):419-421.

[12] 侯炜,杨占秋,陈科力,等.大黄有效成分抗单纯疱疹病毒作用的实验研究[J].药物分析杂志,2003,4(23):259-264.

[13] 刘钊,杨占秋,肖红,等.中药大黄抗柯萨奇病毒作用的实验研究[J].中南民族大学学报(自然科学版),2005,24(4):34-38.

[14] 王志玉,许斌,宋艳艳,等.大黄乙醇提取物的体外抗新城疫病毒作用[J].山东医科大学学报,2002,40(1):26-28.

[15] 梁荣感,刘卫兵,李丽亚,等.大黄蒽醌类化合物体外抗呼吸道合胞病毒作用的研究[J].广西医科大学学报,2007,24(2):280-281.

[16] 贺凤兰,刘强,卫飞,等.大黄提取物和大黄素体外抗轮状病毒的实验研究[J].中国病毒病杂志,2013,3(2):112-116.

[17] Kim T C,Kang S Y,Jung K K,et al.Antiviral activities of extracts isolated from Terminalis chebula Retz.,Sanguisorba of ficinalis L.,Rubus coreanus Miq.and Rheum palmatum L.against hepatitis B virus[J].Phytother Res,2001,15(8):718-720.

[18] 宣华,邢德坤,王殿派,等.应用猪胎肠单层细胞培养猪流行性腹泻病毒的研究[J].中国人民解放军兽医大学学报,1984,4(3):202-207.

[19] Hofmann M,Wyler R.Quantitation,biological and physicochemical properties of cell culture-adapted porcine epidemic diarrhea coronavirus (PEDV)[J].Vet Microbiol,1989,20:131-142.

[20] Kang T J,Yang M S.Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants[J].BMC Biotech,2004,4:20.

[21] 李宝贤.猪流行性腹泻病毒细胞感染受体的生物学特性鉴定[D].哈尔滨:东北农业大学,2007.

[22] 孙东波.猪流行性腹泻病毒S蛋白抗原表位鉴定及受体结合域的初步筛选[D].北京:中国农业科学院,2008.

[23] 程力惠,李晓蒙.大黄中大黄酸提取工艺研究[J].亚热带植物科学,2014,4(1):15-18.

Study on Anti-viral Effect of Rhubarb on Porcine Epidemic Diarrhea Virus in vitro

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HUO Mingkai, GUAN Feihu, QIU Runhui, WEI Chunyan, YU Haitao, ZHU Jiale, ZHANG Hui. Study on the Mechanism of DDX1 Inhibiting Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1556-1566.
[2]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.
[3]	SONG Shiying, GUO Weilu, XIA Xuefeng, ZHANG Xue, BI Zhenwei, ZHANG Xuehan, FAN Baochao, DONG Hailong, LI Bin. Expression and Purification of Porcine IFN-δ5 and Its Effect Analysis Against PEDV Infection [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3171-3179.
[4]	LIU Yutong, YAO Yanting, LI Ruiling, HAN Wenqi, SHI Ting, WANG Yidan, REN Yupeng. Analysis of Genetic Variation of Porcine Epidemic Diarrhea Virus in Parts of Southwest China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2279-2290.
[5]	ZHANG Mingliang, GENG Yachun, LIAN Kaiqi, MA Lei, CHANG Ying, WANG Shuangshan, ZHANG Fuliang. Cloning,Bioinformatics Analysis and Truncated Fragment Expression for Truncated M Gene of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1479-1487.
[6]	ZHANG Tianai, LI Tingting, TAO Xiaoli, LI Tengfei, LIN Jiafeng, HU Siman, LIU Wenxiu, TONG Wei, LI Yonggang. Prokaryotic Expression of Porcine Epidemic Diarrhea Virus S Protein and Preparation and Identification of Its Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4383-4391.
[7]	BAI Weiqin, KA Chula, WU Zhiyong, MIAO Miao, TA Na, GE Riletu. Expression and Identification of B-cell Epitope Embedded S-layer Protein of Porcine Epidemic Diarrhea Virus S2 Gene in Lactobacillus paracasei [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4004-4011.
[8]	LI Jiesen, SUN Ronghang, KUANG Yanqi, CHEN Luman, LIU Qing, GUO Xiaofeng. Optimal Expression of Porcine Epidemic Diarrhea Virus S1D Protein and Preparation of Its Monoclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4641-4651.
[9]	WANG Zhiping, ZHANG Yunjing, SUN Yujie, XU Xin, WANG Zhiyan, HUANG Baicheng, TIAN Kegong. Development and Evaluation of an Indirect ELISA for Detection of Porcine Epidemic Diarrhea Virus IgA in Pig Serum and Milk Captured by Monoclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2248-2255.
[10]	LU Ying, SU Xueli, DU Chen, WANG Ruomu, ZHONG Lian, CHEN Ying, WEI Zuzhang, HUANG Weijian, OUYANG Kang. Reverse Genetics and Its Research Progress of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1694-1701.
[11]	SHEN Xuehuai, ZHAI Yinjian, YIN Lei, PAN Xiaocheng, ZHANG Danjun, ZHAO Ruihong, DAI Yin, HU Xiaomiao, ZHOU Xueli, HOU Hongyan. Culture of Piglet Intestinal Epithelial Cells in vitro and the Infectivity of Porcine Epidemic Diarrhea Virus on the Cells [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4051-4058.
[12]	XIANG Meng, ZHANG Huanrong, REN Yupeng, ZHANG Keke. Sequencing and Genetic Variation Analysis of the Whole Gene of Epidemic Strain of PEDV in Pengzhou,Sichuan Province [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 209-220.
[13]	JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, ZHANG Zhiqing, CHEN Songchang, DENG Shunzhou. Molecular Epidemiological Investigation of Porcine Epidemic Diarrhea Virus in Jiangxi Province in 2017 [J]. , 2019, 46(5): 1474-1482.
[14]	HE Haijian, WU Yuan, WANG Luyan, LI Qunjin, BA Shaobo, SHI Lin, SHAO Chunyan, SUN Jing, JIANG Sheng, WANG Xiaodu. Eukaryotic Expression of Recombinant Baculovirus of PEDV Isolated Strain S1 Gene [J]. , 2019, 46(3): 832-839.
[15]	LEI Dan, LI Anqi, LUO Suxian, YE Yu, SONG Deping, TANG Yuxin. Preparation, Purification and Activity Factors Analysis of IgY Against Variant of Porcine Epidemic Diarrhea Virus [J]. , 2018, 45(8): 2293-2302.