多糖作为兽用疫苗佐剂作用机制的研究进展

doi:10.16431/j.cnki.1671-7236.2022.05.030

Abstract

Abstract: Polysaccharide can promote immune regulation with multi-target,multi-function and multi-factor effects,so it can be widely used as an adjuvant of veterinary vaccine.As vaccine adjuvant,polysaccharide can improve the index of immune organs such as spleen,thymus and bursa of Fabricius of immunized animals,enhance phagocytic activity and antigen presentation ability of macrophages,enhance the killing ability of NK cells and promote the maturation and differentiation of dendritic cells and enhance the ability of antigen uptake,affect the type and number of T cell subsets and change the type of Th1 and Th2 immune response,promote the proliferation of B lymphocytes and stimulate the production of cytokines and antibodies.As a ligand,polysaccharide mainly binds with receptor molecules such as Toll like receptor (TLR),mannose receptor (MR),C-type lectins receptor (CLR),scavenger receptor (SR) and so on to activate downstream signal pathways and exert the above immune activities.In this paper,the mechanism of polysaccharide as an adjuvant of veterinary vaccine was discussed,in order to provide a reference for the further development and application of polysaccharide.

Key words: polysaccharide; adjuvant; receptor; immunity

CLC Number:

S853.74

SHI Qian, XU Xinfeng, LI Xuewen, XIAO Yuanyuan, BAI Meihua, ZHANG Fuchun. Research Progress on the Mechanism of Polysaccharide as Adjuvant of Veterinary Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1895-1907.

References

[1] 苏建青,刘学彬,张晓云,等.植物多糖免疫调节功能研究进展[J].湖北农业科学,2018,57(11):10-13. SU J Q,LIU X B,ZHANG X Y,et al.Research progress in immunomodulating functions of plant polysaccharide[J].Hubei Agricultural Sciences,2018,57(11):10-13.(in Chinese)
[2] 杨琳,傅哲彦,吕正兵,等.免疫佐剂分类及作用机制[J].中国生物工程杂志,2019,39(5):114-119. YANG L,FU Z Y,LYU Z B,et al.Classification and mechanism of immunoadjuvants[J].China Biotechnology,2019,39(5):114-119.(in Chinese)
[3] 崔倩倩,周志勇,刘朝奇,等.植物多糖作为免疫佐剂的研究进展[J].时珍国医国药,2015,26(4):970-972. CUI Q Q,ZHOU Z Y,LIU C Q,et al.Development in plant-derived polysaccharides as immune adjuvant research[J].Lishizhen Medicine and Materia Medica,2015,26(4):970-972.(in Chinese)
[4] 陈亚楠,李美锋,董艳敏,等.中药多糖免疫调节作用研究进展[J].国际中医中药杂志,2016,38(9):855-858. CHEN Y N,LI M F,DONG Y M,et al.The research progress in immune regulation of polysaccharides of traditional Chinese medicine[J].International Journal of Traditional Chinese Medicine,2016,38(9):855-858.(in Chinese)
[5] 郭林霞,马可为,冯志华,等.枣多糖对免疫抑制蛋雏鸡生长性能和免疫指标的影响[J].中国畜牧兽医,2021,48(9):3264-3272. GUO L X,MA K W,FENG Z H,et al.Effects of jujube polysaccharide on growth performance and immune indexes of immunosuppressive egg-type chicks[J].China Animal Husbandry & Veterinary Medicine,2021,48(9):3264-3272.(in Chinese)
[6] 孔晨先,肖波,柳全文,等.山药多糖对动物机体免疫调节作用的Meta分析[J].食品研究与开发,2021,42(19):206-213. KONG C X,XIAO B,LIU Q W,et al.Meta-analysis of the immunomodulatory effect of Chinese yam (Dioscorea opposita rhizoma) polysaccharides on the animal body[J].Food Research and Development,2021,42(19):206-213.(in Chinese)
[7] 李钦,胡继宏,高博,等.黄芪多糖在免疫调节方面的最新研究进展[J].中国实验方剂学杂志,2017,23(2):199-206. LI Q,HU J H,GAO B,et al.Advances on immunoregulation effect of Astragalus polysaccharides[J].Chinese Journal of Experimental Prescriptions,2017,23(2):199-206.(in Chinese)
[8] ENGEL A L,SUN G C,GAD E,et al.Protein-bound polysaccharide activates dendritic cells and enhances OVA-specific T cell response as vaccine adjuvant[J].Immunobiology,2013,218(12):1468-1476.
[9] 吴鑫平,李佳,郝艳艳,等.植物多糖对巨噬细胞的调控及机制研究进展[J].细胞与分子免疫学杂志,2019,35(5):473-478. WU X P,LI J,HAO Y Y,et al.Research progress on regulation and mechanism of plant polysaccharides on macrophages[J].Chinese Journal of Cellular and Molecular Immunology,2019,35(5):473-478.(in Chinese)
[10] 秦延军,杨树宝,孙朋,等.中药多糖对巨噬细胞和树突状细胞免疫调节作用的研究进展[J].中国兽药杂志,2018,52(2):72-77. QIN Y J,YANG S B,SUN P,et al.Effects of polysaccharides in Chinese medicine on the immunomodulator effect of macrophage and dendritic cell[J].Chinese Journal of Veterinary Drug,2018,52(2):72-77.(in Chinese)
[11] 杜海东,邢媛媛,金晓,等.植物多糖对动物免疫细胞的影响及调节机制研究进展[J].饲料研究,2021,44(2):117-121. DU H D,XING Y Y,JIN X,et al.Research progress on effect of plant polysaccharides on animal immune cells and the action mechanism[J].Feed Research,2021,44(2):117-121.(in Chinese)
[12] YANG S,DONG W,LI G,et al.A recombinant vaccine of Riemerella anatipestifer OmpA fused with duck IgY Fc and Schisandra chinensis polysaccharide adjuvant enhance protective immune response[J].Microbial Pathogenesis,2019,136:103707.
[13] 蔡琨,卢芳国,杨娟,等.仙茅多糖作为流感疫苗佐剂对小鼠巨噬细胞的免疫增强作用[J].中华中医药杂志,2018,33(4):1344-1347. CAI K,LU F G,YANG J,et al.Immunological enhancement effects of Curculigo orchioides polysaccharide as adjuvant of influenza vaccine on mouse macrophage[J].Chinese Journal of Traditional Chinese Medicine,2018,33(4):1344-1347.(in Chinese)
[14] 卞娅,葛广波,丁侃.中药多糖抗病毒机制研究进展[J].上海中医药大学学报,2021,35(5):89-96. BIAN Y,GE G B,DING K.Research advance on antiviral mechanism of herbal polysaccharides[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2021,35(5):89-96.(in Chinese)
[15] SUN Y,GUO M,FENG Y,et al.Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice[J].Experimental and Therapeutic Medicine,2016,12(6):3773-3777.
[16] DEL ZOTTO G,MARCENARO E,VACCA P,et al.Markers and function of human NK cells in normal and pathological conditions[J].Cytometry Part B-Clinical Cytometry,2017,92(2):100-114.
[17] 谢红艳,张文伟,林丹义,等.黄芪多糖佐剂对脾脏NK细胞及NKT细胞的作用[J].热带医学杂志,2013,13(6):729-732. XIE H Y,ZHANG W W,LIN D Y,et al.The role of NK and NKT cells in APS adjuvant induced immunologic effect[J].Journal of Tropical Medicine,2013,13(6):729-732.(in Chinese)
[18] LI J,LI J,ZHANG F.The immunoregulatory effects of Chinese herbal medicine on the maturation and function of dendritic cells[J].Journal of Ethnopharmacology,2015,171:184-195.
[19] FENG H,FAN J,QIU H,et al.Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice[J].International Journal of Biological Macromolecules,2015,78:405-416.
[20] DU X,ZHAO B,LI J,et al.Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice[J].International Immunopharmacology,2012,14(4):463-470.
[21] KWAK M,YU K,LEE P C,et al.Rehmannia glutinosa polysaccharide functions as a mucosal adjuvant to induce dendritic cell activation in mediastinal lymphnode[J].International Journal of Biological Macromolecules,2018,120(Pt B):1618-1623.
[22] 彭娟娟,李向敏,王涓,等.食药用真菌多糖对肿瘤免疫逃逸调节作用机制研究进展[J].微生物学报,2021,61(9):2594-2606. PENG J J,LI X M,WANG J,et al.Research progress on regulating tumor immune escape mechanism of edible and medicinal fungal polysaccharides[J].Acta Microbiologica Sinica,2021,61(9):2594-2606.(in Chinese)
[23] 罗霞,胡明华,马方励,等.中药多糖对免疫抑制小鼠T细胞亚群影响的差异研究[J].实用医药杂志,2016,33(7):621-624. LUO X,HU M H,MA F L,et al.Differential study on the effect of traditional Chinese medicine polysaccharides on T cell subsets in immunosuppressive mice[J].Journal of Practical Medicine,2016,33(7):621-624.(in Chinese)
[24] 邵珊,王贵平,李春玲,等.黄芪多糖和白花蛇舌草多糖对猪繁殖与呼吸综合征灭活疫苗免疫猪T细胞亚群及抗体水平的影响[J].中国兽医学报,2011,31(8):1196-1199. SHAO S,WANG G P,LI C L,et al.Effects of APS and OPS on T subpopulations in the immune response to Porcine reproductive and respiratory syndrome inactived virus vaccine[J].Chinese Journal of Veterinary Science,2011,31(8):1196-1199.(in Chinese)
[25] WANG D,YANG Y,LI J,et al.Enhancing immune responses to inactivated Foot-and-mouth virus vaccine by a polysaccharide adjuvant of aqueous extracts from Artemisia rupestris L[J].Journal of Veterinary Science,2021,22(3):e30.
[26] ZHAO B,LIAN J,WANG D,et al.Evaluation of aqueous extracts of Cistanche deserticola as a polysaccharide adjuvant for seasonal influenza vaccine in young adult mice[J].Immunology Letters,2019,213(5):1-8.
[27] ZHANG W,CHENG N,WANG Y,et al.Adjuvant activity of PCP-Ⅱ a polysaccharide from Poria cocos on a whole killed Rabies vaccine[J].Virus Research,2019,270(8):197638.
[28] FENG H,MCDONOUGH S P,FAN J,et al.Phosphorylated Radix cyathulae officinalis polysaccharides act as adjuvant via promoting dendritic cell maturation[J].Molecules,2017,22(1):106.
[29] LIU K,YIN Y,ZHANG J,et al.Polysaccharide PCP-Ⅰ isolated from Poria cocos enhances the immunogenicity and protection of an anthrax protective antigen-based vaccine[J].Human Vaccines & Immunotherapeutics,2020,16(7):1699-1707.
[30] 李群芳,武蕾,姜宁鹏.细胞因子的研究概况[J].山东畜牧兽医,2019,40(11):67-69. LI Q F,WU L,JIANG N P.Overview of cytokine research[J].Shandong Animal Husbandry and Veterinary,2019,40(11):67-69.(in Chinese)
[31] 赵怡,文露婷,黄姻,等.山豆根多糖对罗非鱼生长性能和免疫功能的影响[J].中国畜牧兽医,2021,48(10):3635-3643. ZHAO Y,WEN L T,HUANG Y,et al.Effects of Sophora subprosrate polysaccharide on growth performance and immune function of Oreochromis niloticus[J].China Animal Husbandry & Veterinary Medicine,2021,48(10):3635-3643.(in Chinese)
[32] LIU Y,ZHANG S,ZHANG F,et al.Adjuvant activity of Chinese herbal polysaccharides in inactivated veterinary rabies vaccines[J].International Journal of Biological Macromolecules,2012,50(3):598-602.
[33] LIU C,LUO J,XUE R Y,et al.The mucosal adjuvant effect of plant polysaccharides for induction of protective immunity against Helicobacter pylori infection[J].Vaccine,2019,37(8):1053-1061.
[34] HWANG S H,SHIN M S,YOON T J,et al.Immunoadjuvant activity in mice of polysaccharides isolated from the leaves of Panax ginseng C.A.Meyer[J].International Journal of Biological Macromolecules,2018,107(Pt B):2695-2700.
[35] LIU Z,ZHU T,HE J,et al.Adjuvanticity of Ganoderma lucidum polysaccharide liposomes on Porcine circovirus type-Ⅱin mice[J].International Journal of Biological Macromolecules,2019,141:1158-1164.
[36] 梁艳,黄恋,常海艳,等.人参多糖对新甲型H1N1流感病毒灭活疫苗的免疫增强作用[J].激光生物学报,2012,21(1):36-41. LIANG Y,HUANG L,CHANG H Y,et al.Antibody responses induced by inactivated new Influenza A virus(H1N1) vaccine with ginseng polysaccharide as an adjuvant[J].Acta Laser Biology Sinica,2012,21(1):36-41.(in Chinese)
[37] 孟秀彦,楚遵锋,张静静,等.银杏叶多糖对传染性法氏囊超强毒灭活疫苗的免疫调节作用[J].中国兽医学报,2019,39(4):640-645. MENG X Y,CHU Z F,ZHANG J J,et al.Immunomodulatory functions of Ginkgo biloba leaves polysaccharide on vvIBDV vaccine[J].Chinese Journal of Veterinary Science,2019,39(4):640-645.(in Chinese)
[38] LI P,WANG F.Polysaccharides:Candidates of promising vaccine adjuvants[J].Drug Discoveries & Therapeutics,2015,9(2):88-93.
[39] 汤小芳,刘想,胡美华,等.多糖对免疫系统调控的研究进展[J].食品工业科技,2018,39(9):325-331. TANG X F,LIU X,HU M H,et al.Research progress on the immune regulation of polysaccharides related to immune system[J].Science and Technology of Food Industry,2018,39(9):325-331.(in Chinese)
[40] 朱雪坤,杨日芳,孟艳秋.Toll样受体4配体的研究进展[J].中国药理学与毒理学杂志,2016,30(4):389-396. ZHU X K,YANG R F,MENG Y Q.Research progress in ligands of Toll-like receptor 4[J].Chinese Journal of Pharmacology and Toxicology,2016,30(4):389-396.(in Chinese)
[41] ZHANG X,QI C,GUO Y,et al.Toll-like receptor 4-related immunostimulatory polysaccharides:Primary structure,activity relationships,and possible interaction models[J].Carbohydrate Polymers,2016,149:186-206.
[42] 杨斯锦,潘超,朱力,等.Toll样受体2激动剂在疫苗研发中的研究进展[J].安徽农业科学,2020,48(7):7-10. YANG S J,PAN C,ZHU L.Research progress of Toll-like receptor 2 agonists in vaccine development[J].Journal of Anhui Agricultural Sciences,2020,48(7):7-10.(in Chinese)
[43] AHMADISHOAR S,KARIMINIK A.Toll-like receptor 2 and its roles in immune responses against Legionella pneumophila[J].Life Sciences,2017,188:158-162.
[44] 汲晨锋,陈锦瑞,张子依.植物多糖受体研究进展[J].中国药学杂志,2019,54(21):1766-1772. JI C F,CHEN J R,ZHANG Z Y.Research progress of receptors of plant polysaccharide[J].Chinese Pharmaceutical Journal,2019,54(21):1766-1772.(in Chinese)
[45] BROWN G D,WILLMENT J A,WHITEHEAD L.C-type lectins in immunity and homeostasis[J].Nature Reviews Immunology,2018,18(6):374-389.
[46] 徐旋里,周建英.糖类识别受体:甘露糖受体[J].国际病理科学与临床杂志,2006,26(4):351-354. XU X L,ZHOU J Y.Carbohydrate recognition receptor:Mannose receptor[J].International Journal of Pathology and Clinical Medicine,2006,26(4):351-354.(in Chinese)
[47] 刘小玲,曾令兵.甘露糖受体结构、功能、表达和应用研究的新进展[J].水产学杂志,2013,26(1):54-59. LIU X L,ZENG L B.A review of the research advancement of structure,function,expression and application of mannose receptor[J].Chinese Journal of Fisheries,2013,26(1):54-59.(in Chinese)
[48] VAN DER ZANDE H J P,NITSCHE D,SCHLAUTMANN L,et al.The mannose receptor:From endocytic receptor and biomarker to regulator of (meta) inflammation[J].Frontiers in Immunology,2021,14(12):765034.
[49] JIN Y,LI P,WANG F.β-glucans as potential immunoadjuvants:A review on the adjuvanticity,structure-activity relationship and receptor recognition properties[J].Vaccine,2018,36(35):5235-5244.
[50] HOU H,GUO Y,CHANG Q,et al.C-type lectin receptor:Old friend and new player[J].Medicinal Chemistry,2017,13(6):536-543.
[51] WILLMENT J A,BROWN G D.C-type lectin receptors in antifungal immunity[J].Trends in Microbiology,2008,16(1):27-32.
[52] SAIJO S,IKEDA S,YAMABE K,et al.Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans[J].Immunity,2010,32(5):681-691.
[53] VORUP-JENSEN T,JENSEN R K.Structural immunology of complement receptors 3 and 4[J].Frontiers in Immunology,2018,9:2716.
[54] FERREIRA S S,PASSOS C P,MADUREIRA P,et al.Structure-function relationships of immunostimulatory polysaccharides:A review[J].Carbohydrate Polymers,2015,132:378-396.
[55] RYU S,HOWLAND A,SONG B,et al.Scavenger receptor class A to E involved in various cancers[J].Chonnam Medical Journal,2020,56(1):1-5.
[56] SU C H,LU M K,LU T J,et al.A (1→6)-branched (1→4)-β-d-glucan from Grifola frondosa inhibits lipopolysaccharide-induced cytokine production in RAW264.7 macrophages by binding to TLR2 rather than dectin-1 or CR3 receptors[J].Journal Natural Products,2020,83(2):231-242.
[57] WEI W,XIAO H T,BAO W R,et al.TLR-4 may mediate signaling pathways of Astragalus polysaccharide RAP induced cytokine expression of RAW264.7 cells[J].Journal of Ethnopharmacology,2016,179:243-252.
[58] GUO L,XIE J,RUAN Y,et al.Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum[J].International Immunopharmacology,2009,9(10):1175-1182.
[59] RASHID Z M,MORMANN M,STECKHAN K,et al.Polysaccharides from lichen Xanthoria parietina:1,4/1,6-α-D-glucans and a highly branched galactomannan with macrophage stimulating activity via dectin-2 activation[J].International Journal of Biological Macromolecules,2019,134:921-935.
[60] LIAO W,LUO Z,LIU D,et al.Structure characterization of a novel polysaccharide from Dictyophora indusiata and its macrophage immunomodulatory activities[J].Journal of Agricultural and Food Chemistry,2015,63(2):535-544.
[61] DENG C,FU H,SHANG J,et al.Dectin-1 mediates the immunoenhancement effect of the polysaccharide from Dictyophora indusiata[J].International Journal of Biological Macromolecules,2018,109:369-374.
[62] ZHAN Q,WANG Q,LIN R,et al.Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata michx fruit[J].International Journal of Biological Macromolecules,2020,145:1080-1090.
[63] LEE J,LI C,SURAYOT U,et al.Molecular structures,chemical properties and biological activities of polysaccharide from Smilax glabra rhizome[J].International Journal of Biological Macromolecules,2018,120(Pt B):1726-1733.
[64] LEE J B,TANIKAWA T,HAYASHI K,et al.Characterization and biological effects of two polysaccharides isolated from Acanthopanax sciadophylloides[J].Carbohydrate Polymers,2015,116:159-166.
[65] HUANG Z,ZENG Y J,CHEN X,et al.A novel polysaccharide from the roots of Millettia speciosa champ:Preparation,structural characterization and immunomodulatory activity[J].International Journal of Biological Macromolecules,2020,145:547-557.
[66] JIN Y,ZHANG Y,WAN C,et al.Immunomodulatory activity and protective effects of polysaccharide from Eupatorium adenophorum leaf extract on highly pathogenic H5N1 influenza infection[J].Evidence-Based Complementary and Alternative Medicine,2013,2013:194976.
[67] KIM H W,SHIN M S,LEE S J,et al.Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley[J].International Journal of Biological Macromolecules,2019,131:1084-1091.
[68] MINATO K I,LAAN L C,VAN DIE I,et al.Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation[J].International Journal of Biological Macromolecules,2019,122:705-712.
[69] DONG Z,ZHANG M,LI H,et al.Structural characterization and immunomodulatory activity of a novel polysaccharide from Pueraria lobata (Willd.) Ohwi root[J].International Journal of Biological Macromolecules,2020,154:1556-1564.
[70] LI W J,TANG X F,SHUAI X X,et al.Mannose receptor mediates the immune response to Ganoderma atrum polysaccharides in macrophages[J].Journal of Agricultural and Food Chemistry,2017,65(2):348-357.

Research Progress on the Mechanism of Polysaccharide as Adjuvant of Veterinary Vaccine

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