羊源与骆驼源羊口疮病毒免疫相关基因的比较分析

doi:10.16431/j.cnki.1671-7236.2020.12.003

Abstract

Abstract: The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.

Key words: Orf virus (ORFV); sheep; camels; immune related gene

CLC Number:

S852.65⁺4

DING Xuedong, WANG Guohua, PAN Xiangchen, LIU Dongdong, LUO Xuedong, MA Yuan, LI Maolin, CUI Qi, ZHANG Qijin. Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3815-3824.

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Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

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