寒泊羊脂联素基因表达及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.12.002

Abstract

Abstract: The aim of this study was to investigate the expression and biological characteristics of adiponectin (ADIPOQ) gene in longissimus dorsi muscle of Hanper sheep at different months of age.The ADIPOQ gene mRNA expression was detected in longissimus dorsi muscle of Hanper sheep at 3 different growth periods (1,7 and 13 months old) by Real-time quantitative PCR.The bioinformatics method was used to analyze the nucleotide and amino acid sequences of ADIPOQ gene,protein characteristics and network interaction protein.The results showed that ADIPOQ gene was expressed in longissimus dorsi muscle of Hanper sheep,which showed an increasing trend with the growth of the months age without significant difference (P>0.05).There was higher homology of ADIPOQ gene with Capra hircus (98.60%,99.00%) and Bos taurus (98.60%,87.00%) in nucleotide and amino acid sequences.The results of physical and chemical properties analysis showed that the molecular formula of ADIPOQ protein was C₁₁₇₂H₁₇₇₆N₃₁₄O₃₄₉S₅,the molecular mass was 26.00 ku,and the theoretical isoelectric point (pI) was 5.88,which suggested that the protein was acidic.The average value of ADIPOQ hydrophilic protein (GRAVY) was －0.403,which indicated that the protein was hydrophilic and didn’t belong to transmembrane protein.In addition,the signal peptide sequence was Met¹-Ala¹⁷,and the cleavage site was between Ala¹⁷ and Arg¹⁸.ADIPOQ existed collagen structure domain and C1Q conserved structure domain at position 45－101 and 101－237,respectively.The secondary structure mainly composed of random coil (66.95%).The construction of protein interaction network indicated that ADIPOQ might interact with ADIPOR1,ADIPOR2,CDH13 and LEP.The results provided a theoretical basis for further exploring the molecular biological functions of ADIPOQ gene in the process of muscle development in Hanper sheep.

Key words: Hanper sheep; ADIPOQ gene; expression; bioinformatics

CLC Number:

Q786

LIU Aiju, ZHAO Juan, ZHANG Xin, ZHOU Rongyan, TIAN Shujun, BAI Ying, CHEN Xiaoyong. Expression and Bioinformatic Analysis of ADIPOQ Gene in Hanper Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3805-3814.

References

[1] 李雪梅,薛科,仲涛,等.藏山羊脂联素基因的克隆与表达分析[J].中国畜牧兽医,2014,41(6):23-28. LI X M,XUE K,ZHONG T,et al.Clone and expression analysis of ADIPOQ gene in Tibetan goat[J].China Animal Husbandry & Veterinary Medicine,2014,41(6):23-28.(in Chinese)
[2] 安清明,周辉通,刘秀,等.ADIPOQ基因单体型与不同性别绵羊生长性状的关联分析[J].畜牧兽医学报,2018,49(4):173-180. AN Q M,ZHOU H T,LIU X,et al.Effect of haplotypes of ADIPOQ on growth traits in ovine with different genders[J].Chinese Journal of Animal and Veterinary Sciences,2018,49(4):173-180.(in Chinese)
[3] KADOWAKI T,YAMAUCHI T,KUBOTA N,et al.Adiponectin and adiponectin receptors in obesity-linked insulin resistance[J]. Novartis Foundation Symposium,2007,286:164-176.
[4] YAMAUCHI T,NIO Y,MAKI T,et al.Targeted disruption of ADIPOR1 and ADIPOR2 causes abrogation of adiponectin binding and metabolic actions[J].Nature Medicine,2007,13(3):332-339.
[5] YAMAUCHI T,KAMON J,ITO Y,et al.Cloning of adiponectin receptors that mediate antidiabetic metabolic effects[J]. Nature,2007,423(6941):762.
[6] HOLLAND W L,XIA J Y,JOHNSON J A,et al.Inducible overexpression of adiponectin receptors highlight the roles of adiponectin-induced ceramidase signaling in lipid and glucose homeostasis[J].Molecular Metabolism,2017,6(3):267-275.
[7] MARTINEZ-HUENCHULLAN S F,TAM C S,BAN L A,et al.Skeletal muscle adiponectin induction in obesity and exercise[J].Metabolism-clinical and Experimental,2020,102:154008.
[8] FILIPPI E,SENTINELLI F,TRISCHITTA V,et al.Association of the human adiponectin gene and insulin resistance[J].European Journal of Human Genetics,2004,12(3):199-205.
[9] MORSCI N S,SCHNABEL R D,TAYLOR J F.Association analysis of adiponectin and somatostatin polymorphisms on BTA1 with growth and carcass traits in Angus cattle[J].Animal Genetics,2006,37(6):554-562.
[10] SHIN S,CHUNG E.Novel SNPs in the bovine ADIPOQ and PPARGC1A genes are associated with carcass traits in Hanwoo (Korean cattle)[J].Molecular Biology Reports,2013,40(7):4651-4660.
[11] AN Q,ZHOU H,HU J,et al.Haplotypes of the ovine adiponectin gene and their association with growth and carcass traits in New Zealand Romney lambs[J].Genes,2017,8(6):10-16.
[12] LISTRAT A,PISSAVY A,MICOL D,et al.Collagens XII and XIV:Two collagen types both associated with bovine muscle and intramuscular lipid metabolism[J].Livestock Science,2016,187:80-86.
[13] WANG P H,KO Y H,LIU B H,et al.The expre-ssion of porcine adiponectin and stearoyl coenzyme a desaturase genes in differentiating adipocytes[J].Asian-Australasian Journal of Animal Sciences,2004,17(5):588-593.
[14] DING S T,LIU B H,KO Y H.Cloning and expression of porcine adiponectin and adiponectin receptor 1 and 2 genes in pigs[J].Journal of Animal Science,2004,82(11):3162-3174.
[15] 杨树猛,马登录,郭淑珍,等.藏羊脂联素基因多态性及其与产肉性能的相关性分析[J].中国草食动物科学,2014,34(2):5-9. YANG S M,MA D L,GUO S Z,et al.SNPs of adiponectin gene and its relationship with meat traits in Tibetan sheep[J].Chinese Herbivore Science,2014,34(2):5-9.(in Chinese)
[16] 张依裕,李万贵,潘兰兵,等.鸭脂联素基因多态对肉质和血清生化指标的影响[J].基因组学与应用生物学,2014,33(2):342-347. ZHANG Y Y,LI W G,PAN L B,et al.Effect of polymorphism of adiponectin gene on meat quality and serum biochemical levels in ducks[J].Genomics and Applied Biology,2014,33(2):342-347.(in Chinese)
[17] CIESLAK J,FLISIKOWSKA T,SCHNIEKE A,et al.Polymorphisms in the promoter region of the adiponectin (ADIPOQ) gene are presumably associated with transcription level and carcass traits in pigs[J].Animal Genetics,2013,44(3):340-343.
[18] 徐宜清,韩晓.脂联素与胰岛素抵抗研究进展[J].中国优生与遗传杂志,2007,15(4):120-122. XU Y Q,HAN X.Advances in adiponectin and insulin resistance[J].Chinese Journal of Birth Health & Heredity,2007,15(4):120-122.(in Chinese)
[19] CHANG W T,HUANG M C,CHUNG H F,et al.Interleukin-6 gene polymorphisms correlate with the progression of nephropathy in Chinese patients with type 2 diabetes:A prospective cohort study[J].Diabetes Research & Clinical Practice,2016,120:15-23.
[20] HUSSAIN M K,DELI F A,ALGENABI A H A,et al.Adiponectin gene polymorphisms as a predictor for development of type 2 diabetes mellitus in Iraqi population (Under press)[J].Genes,2018,662:12-18.
[21] HSUEH Y M,CHEN W J,LIN Y C,et al.Adiponectin gene polymorphisms and obesity increase the susceptibility to arsenic-related renal cell carcinoma[J].Toxicology and Applied Pharmacology,2018,350:16-23.
[22] ZHANG Z,LI Y,YANG X,et al.Susceptibility of multiple polymorphisms in ADIPOQ,ADIPOR1 and ADIPOR2 genes to myocardial infarction in Han Chinese[J].Genes,2018,658:10-17.
[23] ALLER R,IZAOLA O,PRIMO D,et al.The effect of single-nucleotide polymorphisms at the ADIPOQ gene locus rs1501299 on metabolic parameters after 9 mo of a high-protein/low-carbohydrate versus a standard hypocaloricdiet[J].Nutrition,2019,65:44-49.
[24] PALIT S P,PATEL R,JADEJA S D,et al.A genetic analysis identifies a haplotype at adiponectin locus:Association with obesity and type 2 diabetes[J].Scientific Reports,2020,10(1):1-10
[25] 苗志国,谢红兵,王永强,等.淮南猪与长白猪脂肪代谢酶及脂联素的发育差异[J].贵州农业科学,2018,329(1):69-71. MIAO Z G,XIE H B,WANG Y Q,et al.Development differences of fat metabolism enzymes and serum adiponectin between huainan and landrace pig[J].Guizhou Agricultural Science,2018,329(1):69-71.(in Chinese)
[26] 张萌萌,魏胜娟,王艺如,等.牛肌内前体脂肪细胞的分离培养及分化相关基因的表达规律研究[J].畜牧与兽医,2018,50(5):1-6. ZHANG M M,WEI S J,WANG Y R,et al.Primary culture of and expression of differentiation-related genes in bovine intramuscular preadipocytes[J].Animal Husbandry & Veterinary Medicine,2018,50(5):1-6.(in Chinese)
[27] 张辉,丛立新,张才,等.围产期酮病牛脂联素mRNA和甘油三酯脂肪酶mRNA的表达[J].中国兽医学报,2008,28(4):465-468. ZHANG H,CONG L X,ZHANG C,et al.Expression of ADPN mRNA and HSL mRNA of ketosis-cow in peripartum[J].Chinese Journal of Veterinary Medicine,2008,28(4):465-468.(in Chinese)
[28] 罗宗刚,李祥,李明洲,等.脂联素(ADIPOQ)及其受体基因(ADIPOR1和ADIPOR2)在猪背最长肌和腰大肌中的表达差异[J].农业生物技术学报,2011,19(3):507-512. LUO Z G,LI X,LI M Z,et al.Expression changes of Adiponection (ADIPOQ) and its receptor genes (ADIPOR1 and ADIPOR2) in porcine longissimus dorsi and psoas major muscles[J].Journal of Agricultural Biotechnology,2011,19(3):507-512.(in Chinese)
[29] 杨洋,陈浩林,徐敏,等.贵州黑山羊ADIPOQ基因组织表达分析及脂肪中表达规律研究[J].中国畜牧杂志,2020,14(5):36-41. YANG Y,CHEN H L,XU M,et al.Tissue expression analysis and expression regularity in adipose tissues of ADIPOQ gene in Guizhou Black goat[J].Chinese Journal of Animal Science,2020,14(5):36-41.(in Chinese)
[30] 孟博,范芮铭,栾新红.脂联素及其受体对脂代谢信号转导通路的调控机制[J].动物营养学报,2016,28(10):3059-3063. MENG B,FAN R M,LUAN X H.Regulation mechanism of adiponectin and its receptor on lipid metabolism signal transduction pathways[J].Chinese Journal of Animal Nutrition,2016,28(10):3059-3063.(in Chinese)
[31] 龚环宇,刘振国,张浩晔.巢式甲基化特异性PCR检测肝细胞癌患者血清中RASSF1A和CDH13基因启动子区甲基化[J].生命科学研究,2011,1:60-64. GONG H Y,LIU Z G,ZHANG H Y.Detection of promoter hypermethylation of RASSF1A and CDH13 gene by nested methylation specific polymerase chain reaction in hepatocellular carcinoma patients[J].Life Science Research,2011,1:60-64.(in Chinese)
[32] 罗铮,翁吉梅,封竣琪,等.瘦素基因的研究进展[J].中国畜牧兽医,2018,45(9):2524-2530. LUO Z,WENG J M,FENG J Q,et al.Research advances on leptin gene[J].China Animal Husbandry & Veterinary Medicine,2018,45(9):2524-2530.(in Chinese)

Expression and Bioinformatic Analysis of ADIPOQ Gene in Hanper Sheep

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