血清4型禽腺病毒广西分离株GX2019-010全基因组测序与分析

doi:10.16431/j.cnki.1671-7236.2020.12.001

Abstract

Abstract: To understand the genetic characteristics of fowl adenovirus serotype 4 (FAdV-4) in Guangxi region,the whole-genome sequencing were performed on a FAdV-4 strain GX2019-010 isolated from Guangxi in this study.Genome-wide nucleotide sequences were analyzed for homology alignment and genetic evolution,and the amino acid sequences of the major structural proteins Hexon,Penton and Fiber were further analyzed.The results showed that GX2019-010 had a full length of 43 719 bp and GC content of 54.9%,mainly including 43 potential protein coding regions.Genome-wide nucleotide homology comparisons revealed that GX2019-010 was 95.8% homologous to the pathogenic strain MX-SHP95,and 95.0% and 95.2% homologous to non-pathogenic strains ON1 and KR5,respectively.Genetic evolutionary analysis showed that GX2019-010 was in the same branch as the popular strains of FAdV-4 in China in recent years,but not in the same branch as the foreign strains of FAdV-4.This results indicated that there were genetic differences in FAdV-4.The main differences was at the right end of the genome and all domestic strains isolated in China were 1 966 bp missing including ORF19,ORF48 and ORF27 genes.It showed that FAdV-4 popular in China was territorial.Analysis of the amino acid sequences of the major structural proteins of Hexon,Penton and Fiber revealed that all of the three major structural proteins had mutations in amino acid sites,of which Fiber-2 had the most mutations.This study enriched the gene pool of FAdV-4 in Guangxi region and laid the foundation for future research on the prevention and control of HHS and epidemiological investigation.

Key words: fowl adenovirus serotype 4(FAdV-4); whole genome sequencing; evolutionary analysis

CLC Number:

S852.65⁺7

LUAN Yongjiao, XIE Zhixun, WANG Sheng, LUO Sisi, ZHANG Lei, XIE Liji, XIE Zhiqin, DENG Xianwen, ZHANG Minxiu, ZHANG Yanfang, ZENG Tingting, FAN Qing. Whole-genome Sequencing and Analysis of Fowl Adenovirus Serotype 4 Strain GX2019-010 Isolated from Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3793-3804.

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Whole-genome Sequencing and Analysis of Fowl Adenovirus Serotype 4 Strain GX2019-010 Isolated from Guangxi

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