1株犬副流感病毒的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2019.03.030

Abstract

Abstract:

The aim of this study was to isolate and identify the canine parainfluenza virus (CPIV),and analyze its biological characteristics.CPIV positive canine lung tissue was inoculated into Vero cells.CPE were observed after four blind passages,then the cell culture fluid after 72 h was assayed for further RT-PCR identification,electron microscopy,HA and biological characteristic experiments.At the same time,N gene of CPIV was amplified,and phylogenetic tree was constructed.The result showed that one CPIV was successfully isolated and named as CPIV-BJ01.The result of RT-PCR showed that there was a specific target fragment of 534 bp.The electron microscopic observation showed that the virus were circular,enveloped and between 80 to 200 nm in diameter.It could agglutinate 1% porcine erythrocytes at 4 or 37 ℃.The virus was sensitive to high temperature and UV irradiation.The one-step growth curve showed that the virus replicated at a high speed between 12 to 48 h.Compared with 19 representative CPIV N genes,the nucleotide sequence homology analysis was 97.1% to 99.8%.The CPIV-BJ01 strain was closely genetically related to strain PIV5 1168-1 (GenBank accession No.:KC237064.1) and PIV5 ZJQ 221(GenBank accession No.:KX100034.1).

Key words: canine parainfluenza virus (CPIV); isolation and identification; biological characteristics; evolutionary analysis

CLC Number:

S852.65⁺9.5

LIU Chang, QIN Tong, LIANG Lin, YOU Xinyue, WANG Chunfeng, QIAN Aidong, LI Jinxiang, CUI Shangjin. Isolation,Identification and Biological Characterization Analysis of One Strain Canine Parainfluenza Virus[J]. , 2019, 46(3): 891-897.

References

[1] LIU C,LI X,ZHANG J P,et al.Isolation and genomic characterization of a canine parainfluenza virus type 5 strain in China[J].Archives of Virology,2017,162(8):2337-2344.
[2] ELLIS J,ANSEEUW E,GOW S,et al.Seroepidemiology of respiratory canine coronavirus,canine parainfluenza virus,and bordetella bronchiseptica infections in urban dogs in a humane shelter and in rural dogs in small communities[J].Canadian Veterinary Journal,2011,52(8):861-868.
[3] KRAUS T A,GARAZA L,HORVATH C M,et al.Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection[J].Virology,2007,371(1):196-205.
[4] 翟俊琼,周霞,邹舒展,等.虎源、小熊猫源副流感病毒5型的鉴定及F基因遗传进化分析[J].中国兽医学报,2018,38(4):700-707. ZHAI J Q,ZHOU X,ZOU S Z,et al.Identification of the parainfluenza virus type 5 from tiger source,red panda source and analysis of F gene genetic evolution in genus[J].Chinese Journal of Veterinary Science,2015,38(4):700-707.(in Chinese)
[5] 孙贺廷,夏咸柱,高玉伟,等.虎血清中犬副流感病毒的抗体流行病学调查研究[J].养犬,2003,1(3):7-10. SUN H T,XIA X Z,GAO Y W,et al.Anti-epidemiological investigation of major parainfluenza virus in tiger serum[J].Dog Breeding,2003,1(3):7-10.(in Chinese)
[6] MOCHIZUKI M,YACHI A,OHSHIMA T,et al.Etiologic study of upper respiratory infections of household dogs[J].Virology,2008,70(6):563-569.
[7] 胡晓霞.三种主要犬呼吸道病毒多重PCR方法的建立和应用[D].武汉:华中农业大学,2013. HU X X.Establishment and application of the multiplex PCR for three main canine respiratory tract viruses[D].Wuhan:Huazhong Agricultural University,2013.(in Chinese)
[8] ZHAI J Q,ZHAI S L,LIN T,et al.First complete genome sequence of parainfluenza virus 5 isolated from lesser panda[J].Archives of Virology,2017,162(5):1413-1418.
[9] LEE Y N,LEE C.Complete genome sequence of a novel porcine parainfluenza virus 5 isolate in Korea[J].Archives of Virology,2013,158(8):1765-1772.
[10] BARIK S.Control of nonsegmented negative-strand RNA virus replication by siRNA[J].Virus Research,2004,102(1):27-35.
[11] 仲晓丽.犬副流感病毒单克隆抗体的制备及单链抗体蛋白的表达和活性鉴定[D].武汉:华中农业大学,2015. ZHONG X L.Preparation of monoclonal antibody against CPIV,expression and activity analysis of single chain Fv[D].Wuhan:Huazhong Agricultural University,2015.(in Chinese)
[12] SCHULZ B S,KURZ S,WEBER K,et al.Detection of respiratory viruses and Bordetella bronchiseptica in dogs with acute respiratory tract infections[J].The Veterinary Journal,2014,201(3):365-369.
[13] 闫喜军.犬副流感病毒进化分析及核酸疫苗和犬腺病毒重组疫苗的研究[D].长春:吉林大学,2007. YAN X J.Evolutionary of canine parainfluenza virus and experimental study on CPIV DNA vaccine and recombinant canine adenovirus type-2 vaccine[D].Changchun:Jilin University,2007.(in Chinese)
[14] DAMIAN M,MORALES E,SALAS G,et al.Immunohistochemical detection of antigens of distemper,adenovirus and parainfluenza viruses in domestic dogs with pneumonia[J].Journal Comparative Pathology,2005,133(6):289-293.
[15] MONTEIRO F L,CARQNELUTTI J F,MARTINS M,et al.Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions[J].Virology,2016,47(4):876-881.
[16] 刘腾飞,马素贞,申卫红,等.犬副流感病毒NP基因的克隆与原核表达[J].新疆农业大学学报,2010,32(2):146-150. LIU T F,MA S Z,SHEN W H,et al.Cloning and prokaryotic expression of NP gene of canine parainfluenza virus[J].Journal of Xinjiang Agricultural University,2010,32(2):146-150.(in Chinese)
[17] CANINO B,VITO M.Canine respiratory viruses[J].Veterinary Research,2007,38(2):355-373.
[18] CHATIZANDREOU N,STOCK N,YOUNG N,et al.Relationships and host range of human,canine,simian and porcine isolates of simian virus 5(parainfluenza virus 5)[J].Journal General Virology,2004,85(10):3007-3016.
[19] SCHULLZ R D.Duration of immunity for canine and feline vaccines:A review[J].Veterinary Microbiology,2006,117(1):75-79.
[20] RUCH G R,MOROFF S,LAPPIN M R.Adenovirus 2,Bordetella bronchiseptica,and parainfluenza molecular diagnostic assay results in puppies after vaccination with modified live vaccines[J].Journal of Veterinary Internal Medicine,2016,30(1):164-166.
[21] 李长磊.犬副流感病毒的分离鉴定及结构蛋白基因的克隆与序列分析[D].长春:吉林农业大学,2013. LI C L.Isolation and identification of canine parainfluenza viruses and cloning and sequence analysis of it's structural protein genes[D].Changchun:Jilin Agricultural University,2013.(in Chinese)
[22] 刘腾飞.犬副流感病毒的检测和NP基因的克隆、表达及其重组蛋白的免疫学活性的研究[D].乌鲁木齐:新疆农业大学,2010. LIU T F.Detection of canine parainfluenza virus and cloning and expression of CPIV-NP gene and immunocompetence of its recombinant protein[D].Urumqi:Xinjiang Agricultural University,2010.(in Chinese)
[23] 高菽蔓,靳红亮,张守峰,等.犬副流感病毒CC-14株全基因组测序及分析[J].中国预防兽医学报,2015,37(10):802-804. GAO S M,JIN H L,ZHANG S F,et al.Whole genome sequencing and analysis of canine parainfluenza virus CC-14 strain[J].Chinese Journal of Preventive Veterinary Medicine,2015,37(10):802-804.(in Chinese)

Isolation,Identification and Biological Characterization Analysis of One Strain Canine Parainfluenza Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[2]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[3]	WANG Leping, PAN Yan, TAN Yizhou, LI Lei, HAN Kaiou, CAO Yajie, LI Jun, ZHOU Yuqing, LIAO Yuying, MA Dongxin, GONG Yu, PENG Hao, WANG Xiaoye. Isolation,Identification and Genome Analysis of Escherichia coli Phage vB_EcoP_GN07 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1522-1531.
[4]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[5]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[6]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.
[7]	CHENG Longfei, LIAO Weilian, CHEN Hongmei, JIANG Nansong, FU Qiuling, WAN Chunhe, LIU Rongchang, SHI Shaohua, HUANG Yu, FU Guanghua. Screening and Biological Characteristics of Broad-spectrum Riemerella anatipestifer Phage [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1169-1176.
[8]	GAN Lu, ZHENG Huizhen, NUO Mingdalai, LIU Yan, JIN Min, HE Wenwen, WEN Licui, LI Yongchang, GAILIKE·Bayinchahan. Propagation of Trypanosoma evansi Yili Strain and Cloning, Expression and Bioinformatics Analysis of Its PFR Gene [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 469-478.
[9]	LIU Bin, LI Meng, XI Qianyun, SUN Jiajie, LUO Junyi, ZHANG Yongliang, CHEN Ting. Research Progress on Biological Characteristics of Hermetia illucens L. and Its Application in Poultry Production [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 586-597.
[10]	YIN Yangyan, PAN Yan, LI Xiaoning, LI Jun, LIAO Yuying, LI Changting, WEI Tianchao, PENG Hao. Study on Biological Characteristics and Antibacterial Activity in vitro of a Lactobacillus plantarum Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 666-673.
[11]	WANG Bingyi, MA Hongcai, ZOU Minghao, FAN Shijie, YUAN Zhenjie, Labaciren, DONG Hailong, ZENG Jiangyong. Isolation,Identification and Drug Resistance Analysis of Streptococcus from Yak [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 745-753.
[12]	ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.
[13]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[14]	WEI Jin, WANG Jia, ZHAO Haoran, LIU Bo, LIU Yuanjie, YAO Wensheng, LIU Yan, MA Xin. Isolation, Identification and Antimicrobial Susceptibility of Mycoplasma gallisepticum from Different Areas [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 368-376.
[15]	YANG Fumei, YANG Linfu, ZENG Cheng, YANG Zhi, WANG Kang, DUAN Gang, DAI Feiyan. Isolation,Identification and Biological Characterization Analysis of a Strain of Carnobacterium maltaromaticum from Barking Deer [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 398-407.