猪源牛病毒性腹泻病毒JLS-01株的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2019.02.027

Abstract

Abstract:

In order to understand the molecular characteristics and pathogenicity of bovine viral diarrhea virus (BVDV),in this study,RT-PCR was used to detect BVDV nucleic acid positive in piglets with severe diarrhea symptoms in a pig farm in Jilin province.The treated BVDV positive samples were inoculated into MDBK cells,and one strain of virus was isolated and designated as BVDV JLS-01.The molecular evolution characteristics were analyzed by immunofluorescence detection and 5'UTR and N^pro RT-PCR amplification.The results showed that the isolated strain was blindly transmitted to MDBK cells for 8 generations without cytopathic effect,and showed a positive fluorescent signal in the immunofluorescence assay.5'UTR and N^pro fragments of 280 and 735 bp in size were obtained by RT-PCR amplification.The genetic evolution analysis of the 5'UTR and N^pro sequences of BVDV JLS-01 strain showed that it was closely related to LN-1 and ZM-95,and the homology with the LN-1 of bovine source strain was 99.3%,suggesting that the strain might be derived from a bovine strain.BVDV and classical swine fever virus (CSFV) antibody negative pigs were artificially infected with BVDV JLS-01 strain F8 cell culture medium,the infected pigs did not show significant elevation of body temperature,but the number of white blood cells decreased,and the virus strain was isolated from the leukocyte extract of infected pigs,indicating that the virus strain had certain pathogenicity.The successful isolation of this strain was of great significance for further research on the epidemiological investigation and pathogenesis of BVDV.

Key words: bovine viral diarrhea virus (BVDV) from porcince; evolutionary analysis; isolation and identification; pathogenicity

CLC Number:

S852.65⁺3

ZHANG Shuqin, TAN Bin, LIU Zeyu, WANG Chao, GUO Li, SUN Na, CHENG Shipeng. Isolation,Identification and Pathogenicity Analysis of Bovine Viral Diarrhea Virus JLS-01 Strain from Porcine[J]. , 2019, 46(2): 557-564.

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Isolation,Identification and Pathogenicity Analysis of Bovine Viral Diarrhea Virus JLS-01 Strain from Porcine

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