牛UCP3和MYH1基因启动子在C2C12和3T3-L1细胞中的启动活性分析

doi:10.16431/j.cnki.1671-7236.2020.12.004

Abstract

Abstract: To confirmed the core regions of uncoupling protein 3 (UCP3) and myosin heavy chain 1 (MYH1) genes promoter and the promoter activity in C2C12 and 3T3-L1 cells,PCR,double luciferase and liposome transfection methods were used to amplify of the promoter of UCP3 and MYH1 genes,construct recombinant vectors,and analyze promoter activity in this study.The results showed that pGL3-Basic-UCP3-pro and pGL3-Basic-MYH1-pro were successfully constructed and transfected into C2C12 and 3T3-L1 cells,respectively.The core promoter region of UCP3 and MYH1 genes were UCP3-P6 (－385 to +3 bp) and MYH1-P5 (－255 to +15 bp),respectively.The promoter activity of pGL3-Basic-MYH1-P2 to pGL3-Basic-MYH1-P5 in C2C12 cells were higher than that in 3T3-L1 cells,indicating that the promoter activity of MYH1 gene in C2C12 cells was higher.This study clarified the core region and promoter activity of UCP3 and MYH1 genes promters,provided a scientific basis for further study on the transcriptional regulation mechanism of UCP3 and MYH1 genes.

Key words: cattle; UCP3 gene; MYH1 gene; promoter; C2C12 cells; 3T3-L1 cells

CLC Number:

S823

CHEN Wei, XU Houqiang, CHEN Xiang, QIN Hai, WU Yu, HUANG Mingjie, LU Xianjun. Promoters Activity Analysis of UCP3 and MYH1 Genes of Cattle in C2C12 and 3T3-L1 Cells[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3825-3832.

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Promoters Activity Analysis of UCP3 and MYH1 Genes of Cattle in C2C12 and 3T3-L1 Cells

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