中草药复合添加剂的小鼠急性毒性试验

doi:10.16431/j.cnki.1671-7236.2018.12.032

Abstract

Abstract:

To study the acute toxicity of Chinese herbal medicine compound additive for invigorating qi-blood,liver and kidney,the intragastric administration was adopted in this study,40 SPF Kunming mice were divided into four groups:Control group,low dose group,middle dose group and high dose group,and supplemented with the concentration 0.86 g/mL of Chinese herbal medicine compound additive.The suspension was administered intragastrically at a volume of 0.4 mL/10 g per gavage,twice within 24 h in high dose group (0.4 mL each time) and middle dose group (0.3 mL each time) for 7 d,the control group was given intragastrically 0.4 mL distilled water twice within 24 h for 7 d,and the low dose group was given 0.2 mL,once within 24 h for 7 d.The general condition and death of mice were observed daily.At end of the test,blood was collected from the eyeballs of mice to detect physiological and biochemical indicators.The mice were anatomized,and the organ index was calculated by weighing the heart,liver,spleen,lung and kidney,and pathological sections were made to observe the pathological changes.The results showed that there was no abnormality in mice of experimental groups.Compared with the blank control group,the body weight of mice in middle dose group and high dose group was extremely significantly increased (P<0.01).The liver index and spleen index of the high dose group were extremely significantly higher than other groups (P<0.01).The total number of white blood cells of mice in Chinese herbal medicine compound additive groups were extremely significantly increased (P<0.01),but it was still within the normal range.The blood creatinine was significantly decreased in Chinese herbal medicine compound additive groups (P<0.05),and the urea nitrogen of middle dose group was significantly increased (P<0.05);There was no significant difference in other biochemical indexes among all groups (P>0.05).The results of pathological tissue sections showed that there was no pathological changes in liver,spleen and kidney in all groups.In conclusion,Chinese herbal medicine of invigorating qi-blood,liver and kidney played a significant role in promoting protein metabolism which could significantly improve the body immunity and kidney function.The maximum dose (34.4 g/kg,crude drug) was 92.7 times as much as the amount of clinical adult (0.371 g/kg) daily,proving that the drug was non-toxic and safe.

Key words: Chinese herbal medicine compound additive; mice; acute toxicological test

CLC Number:

S859.3

CUI Yaocheng, MAI Zhanhai, KULAIXIA·Alibieke, WANG Dechao, SHAYA·Nuerlan, ZHANG Aoyuntuya, KUANG Ling. An Experimental of Mice Acute Toxicological Test of Chinese Herbal Medicine Compound Additive[J]. , 2018, 45(12): 3579-3586.

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An Experimental of Mice Acute Toxicological Test of Chinese Herbal Medicine Compound Additive

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