乳酸菌发酵扶正解毒散复合制剂的制备及其在肉鸡上的应用研究

doi:10.16431/j.cnki.1671-7236.2016.04.032

Abstract

Abstract: This study was aimed toproduce compound preparations of Fuzhengjiedusan fermented with Lactic acid bacteria and analyze its effectson growth performancesand immune organ growth of broilers. Firstly, to identify the influence of traditional Chinese medicine on the growth of Lactic acid bacteria, the medium for the growth of Lactic acid bacteria was filled with different proportions of Astragalusmembranaceus, Radix Isatidis, Epimedium and compound Chinese herbal medicine extracts by single factor test. Secondly, to confirm the optimum medium, orthogonal test was used to optimize the culture medium of compound medicine liquid. Finally, 450 one-day-old healthy AA broilers were randomized into three groups, each group had 3 repeats: control group (antibiotics), group Ⅰ(fermented preparation), group Ⅱ (unfermented preparation). The growth and immune organ index were determined in 21 and 42 days after administration. The results showed that the highest viable count of Astragalusmembranaceus, Radix Isatidis, Epimedium and compound medicine liquid groups were increased by 261.64%, 505.48%, 156.16% and 564.38%, respectively, compared with control group. The optimum medium composition was compound medicine liquid 100 mL, brown sugar 2.0 g, peptone 0.3 g, soybean meal 2.0 g. The viable count of fermentation liquid was 7.13×10⁹ CFU/mL.The production results showed that in period of 1~42 days, the daily weight gain of group Ⅰ and Ⅱ were separately increased by 17.84% and 8.22% (P<0.05) than that of the control. What's more, the feed conversion rate was reduced by 10.88% (P<0.05). The average daily feed intake was significantly higher than control group (P<0.05). At 21 and 42 days, the spleen index of group Ⅰ were improved by 11.04% and 13.58% (P<0.05), respectively. The bursal index were raised by 31.30% and 26.23% (P<0.05), respectively. However, the spleen index of group Ⅱ were improved by 7.79% and 7.41% (P<0.05), respectively. The bursal index were raised by 15.65% and 11.48% (P<0.05), respectively. On the basis of the above results, it could be suggested that Chinese herbal medicine extracts can promote lactic acid bacteria proliferation and compound fermentation liquid can improve growth performance in broilers, as well as immune organ development.

Key words: lactic acid bacteria; compound Chinese herbal medicine; fermentation; broilers

CLC Number:

S811.6

SHI Hong-tao, QIAO Hong-xing, WANG Yong-fen, JIANG Ya-le, BIAN Chuan-zhou. Studies on Preparation and Application of Fuzhengjiedusan Compound Preparation Fermentated by Lactic Acid Bacteria in Broilers[J]. , 2016, 43(4): 1066-1071.

References

[1] Wu B Q,Zhang T.Effects of Bacillus subtilis KD1 on broiler intestinal flora[J].Poultry Science,2011,90(11):2493-2499.
[2] Chen C Y,Tsen H Y.Oral administration of a combination of select lactic acid bacteria strains to reduce the Salmonella invasion and inflammation of broiler chicks[J].Poultry Science,2012,91(9):2139-2147.
[3] 胡元春,张颖,班曼曼,等.扶正解毒散对鸡传染性法氏囊病的治疗效果研究[J].河南畜牧兽医,2013,34(9):45-46.
[4] 吴志勇,林陆山.采用生物工程技术发酵中药获得成功[J].World Science and Technology Modernization of Traditional Chinese Medicine,2002,4(3):12.
[5] 戴万生,赵荣华.发酵法对大黄蒽醌类成分含量的影响[J].云南中医中药杂志,2005,26(1):38-39.
[6] 林燕文,王茂先,陆宝君.枸杞子、决明子对泡菜中乳酸菌生长的影响[J].食品研究与开发,2006,27(4):67-69.
[7] 朱新术,杨志强,李建喜,等.发酵黄芪的乳酸菌的驯化及其与黄芪相互作用研究[J].中国生态学杂志,2008,20(5):450-451.
[8] 王成涛,季宝平,朱桂华,等.五味中草药对乳酸菌生长及保存活力的影响[J].中国生态学杂志,2004,16(2):75-76.
[9] 田慧敏,刘小荣.乳酸菌发酵酸菜及廉价培养基的筛选[J].赤峰学院报,2012,28(10):23-25.
[10] 吕兴萍.黄芪多糖在动物生产中的应用[J].上海畜牧兽医通讯,2011,6:40-41.
[11] 许雪燕,周鹏.板蓝根的药理作用及临床应用[J].海峡药学,2014,26(8):33-35.
[12] 曾庆岳,王云山.淫羊藿药理作用研究进展[J].医药导报,2012,31(4):462-465.
[13] Wang Y,Gu Q.Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers[J].Research in Veterinary Science,2010,89(2):163-167.
[14] 张凯,李建喜,杨志强,等.发酵型黄芪提取物对肉仔鸡生产性能及血液生化指标的影响[J].中国畜牧兽医,2011,38(5):31-34.
[15] 刘观忠,安胜英,王凤霞.发酵中药对肉仔鸡生产性能和血液生化指标的影响[J].饲料工业,2011,32(23):47-49.
[16] 王显慧,刘福柱,牛竹叶,等.VC对肉鸡生产性能、胴体品质、免疫机能和抗氧化特性的影响[J].西北农林科技大学学报,2010,38(2):1-7.
[17] 赵云焕,李迎晓,焦凤超,等.黄芪多糖、益生菌对固始鸡生产性能和免疫效果的影响[J].江西农业科学,2012,40(9):202-203.
[18] 崔新颖,崔新羽,张成义.淫羊藿苷对实验大鼠骨质疏松症的影响[J].北华大学学报:自然科学版,2008,9(2):121-122.

Studies on Preparation and Application of Fuzhengjiedusan Compound Preparation Fermentated by Lactic Acid Bacteria in Broilers

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