高效液相色谱法检测鸡蛋中四环素类药物残留方法研究

doi:10.16431/j.cnki.1671-7236.2016.06.027

Abstract

Abstract: The study was aimed to establish the method of simultaneous detection of four kinds of tetracyclines,including oxytraoycline,tetracycline,chlortetracycline and doxyeycline in eggs by high performance liquid chromatography (HPLC).The eggs samples were detected with HPLC after through the procedure of extraction of 0.1 mol/L Na₂EDTA-Mcllvaine buffered solution,purification of Oasis MAX solid-phase extraction column,gradient elution of taking acetonitrile-0.01 mol/mL trifluoroacetic acid as mobile phase,separation with reverse liquid chromatography.The results showed that the calibration curves of four kinds of tetracyclines were linear correlated in the concentration range of 30 to 1 000 ng/g which the limits of detection was 10 ng/g and the limit of quantification was 50 ng/g.The average recoveries were 67.3% to 81.4%,relative standard deviations of intraday was 2.7% to 14.3%,relative standard deviations of inter-day was 0.9% to 5.0%.This mothod established in this study could simultaneously detect oxytetracycline,tetracycline,chlortetracycline and doxycycline drug residues in egg and it had high sensitivity,accuracy and precision.The method was rapid and simple to operate.

Key words: tetracyclines; residue; eggs; HPLC

CLC Number:

S859.84

WANG Xu-dan, LIU Jing, WANG Geng-nan, ZHANG Dong-xu, LIU Ju-xiang. Residue Detection of Tetracyclines in Eggs by High Performance Liquid Chromatography[J]. , 2016, 43(6): 1579-1584.

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Residue Detection of Tetracyclines in Eggs by High Performance Liquid Chromatography

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