China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (7): 2324-2332.doi: 10.16431/j.cnki.1671-7236.2021.07.006

• Biotechnology • Previous Articles     Next Articles

Construction of OCX-32 Gene Expression Vector and Its Effect on Ca2+ Concentration Lipid Indexes of Chicken Uterine Epithelial Cells

LIAO Chaomei1, WU Lei1, YOU Minfang1, TAN Guanghui1, LI Jiezhang1, LI Wangui2, ZHANG Yiyu1   

  1. 1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. Liupanshui City Grassland Workstation, Liupanshui 553000, China
  • Revised:2020-11-02 Online:2021-07-20 Published:2021-07-15

Abstract: In order to explore the relationship between ovocalyxin-32 (OCX-32) gene overexpression and Ca2+ concentration and lipid indexes of chicken uterine epithelial cells.The Changshun Green-eggshell chicken uterine epithelial cells were isolated and cultured by collagenase Ⅳ method.The expression vector pcDNA3.1(+)+OCX-32+Flag was constructed by homologous recombination method and transfected into uterine epithelial cells.The overexpression of OCX-32 gene,the concentration of Ca2+ and the indexes of lipid were determined and the correlation was analyzed.The results showed that the concentration of total cholesterol (TCHO) in the experimental group was significantly higher than that in the control group (P<0.05),and the concentration of Ca2+ in the control group was significantly higher than that in the experimental group (P<0.05).The OCX-32 gene overexpression was negatively correlated with Ca2+ concentration (P<0.05),and significant positive correlation with triglyceride (TG) concentration (P<0.05),and extremely significant negative correlated with TCHO concentration (P<0.01).Meanwhile,TCHO concentration was extremely significant negative correlated with TG concentration (P<0.01).The results revealed that overexpression of OCX-32 gene could regulate Ca2+ concentration and lipid synthesis and transport in chicken uterine epithelial cells.The results of this study provided experimental basis and data support for further elucidating the regulatory mechanism of lipid and calcium secretion in chicken uterine epithelial cells.

Key words: OCX-32 gene; Changshun Green-eggshell chicken; uterine epithelial cells; Ca2+; lipid indexes

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