玻璃化冷冻对哺乳动物卵母细胞Ca2+的影响机制

doi:10.16431/j.cnki.1671-7236.2016.03.030

Abstract

Abstract: Oocyte cryopreservation is an important part of embryo biotechnologies (e.g.in vitro fertilization,intracytoplasmic sperm injection and somatic cell nuclear transfer),and is of a great significance in conserving the germplasm resources of excellent and endangered animals,accelerating the process of livestock breed improvement.Compared with the conventional freezing method,vitrification has many advantages,such as fast cooling rate and high efficiency,and is widely used in oocyte cryopreservation.However,fertilization and developmental competence of vitrified oocytes are still not ideal,which seriously affects the application of vitrified oocytes.Vitrification leads to increased calcium ion (Ca²⁺) concentration and abnormal Ca²⁺ oscillation pattern in oocytes,which hardens their zona pellucida and disorders their fertilization signal.By integrating previous findings,present paperanalyzed the influence and mechanism of vitrification on intracellular Ca²⁺ concentration and Ca²⁺oscillation pattern,and proposed that extracellular Ca²⁺ influx and intracellular Ca²⁺ release induced by vitrification led to Ca²⁺ increasing in vitrified oocytes,and inositol 1,4,5-triphosphate (IP3) Ⅰ receptor maldistribution and mitochondrial damage led to abnormal Ca²⁺ oscillation pattern in vitrified oocytes.Above information may provide technical reference for researchers to regulate Ca²⁺ concentration and Ca²⁺ oscillation pattern positively in vitrified oocyte,and further improve their fertilization and subsequent developmental competence.

Key words: vitrification; oocytes; Ca²⁺; Ca²⁺ oscillation; influence; mechanism

CLC Number:

S814

WANG Na, HAO Hai-sheng, WANG Hao-yu, DU Wei-hua, QIN Tong, LIU Yan, ZHU Hua-bin, ZHAO Xue-ming. The Influencing Mechanism of Vitrification on the Intracellular Calcium of Mammalian Oocytes[J]. , 2016, 43(3): 768-775.

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The Influencing Mechanism of Vitrification on the Intracellular Calcium of Mammalian Oocytes

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