利用Y染色体AMELY基因分析中国马鹿的遗传多样性

doi:10.16431/j.cnki.1671-7236.2016.03.029

Abstract

Abstract: In order to determine the genetic diversity in Cervus elaphus using AMELY gene in Y chromosome,200 blood samples from Cervus elaphus yarkandensis,Cervus elaphus asiaticus,Cervus elaphus xanthopygus,Cervus elaphus songaricus and Cervus elaphus kansuensis populations were collected and AMELY genes were sequenced in this study.The haplotype diversity of Y chromosome was analyed,phylogenetic tree was built to explore the genetic diversity and the paternal origins about Cervus elaphus.The results showed that:Cervus elaphus yarkandensis had the most variation sites and the highest nucleotide diversity.The genetic distance between Cervus elaphus yarkandensis and other Cervus elaphus were far.6 haplotypes were identified in this study,named as A1,A2,A3,A4,A5 and A6,respectively.Cervu elaphus yarkandensis,Cervus elaphus asiaticus and Cervus elaphus kansuensis had separate haplotype.The NJ and ML phylogenetic trees showed that Cervus elaphus songaricus,Cervus elaphus asiaticus,Cervus elaphus xanthopygus and Cervus elaphus kansuensis clustered together which Cervus elaphus yarkandensis and Cervus elaphus kansuensis were form a department,separately.Cervus elaphus asiaticus,Cervus elaphus xanthopygus and Cervus elaphus yarkandensis clustered into one branches and there might be gene exchange among Cervus elaphus yarkandensis,Cervus elaphus kansuensis and other Cervus elaphus.

Key words: Cervus elaphus; Y chromosome; AMELY gene; paternal origins

CLC Number:

Q786

SU Ying, XING Xiu-mei, SHAO Yuan-chen, WANG Hong-liang, ZHANG Ran-ran, JU Gui-chun. Genetic Diversity Analysis of Cervus elaphus Using AMELY Gene in Y Chromosome[J]. , 2016, 43(3): 761-767.

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Genetic Diversity Analysis of Cervus elaphus Using AMELY Gene in Y Chromosome

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