奶及奶制品中兽药残留高通量检测方法研究进展

doi:10.16431/j.cnki.1671-7236.2020.10.041

Abstract

Abstract: The abuse of veterinary drugs in production may lead to veterinary drug residues in milk and pose a risk to consumer health.As the kinds of veterinary drugs are too many,the detection method for single target presents low efficiency.Whereas the high-throughput detection technology can detect various veterinary drug residues simultaneously,with time and labor saving,and become a research hot spot.In the aspect of sample pretreatment,dispersed solid phase extraction techniques,such as QuEChERS method,are widely used in sample purification due to its features of simplicity,rapidity and low cost.On the other hand,because of its characteristics of fast separation,high sensitivity and accuracy,ultra-high performance liquid chromatography (UPLC) tandem mass spectrometry (MS) has shown better analytical performance and application prospect than high performance liquid chromatography (HPLC).With the application of new technologies and instruments,veterinary drug residue detection has developed rapidly in terms of pretreatment efficiency,instrument sensitivity and high-throughput detection.In this paper,based on recent literatures and publications,the pretreatment techniques and high-throughput detection technology of veterinary drug residues in milk and dairy products were reviewed,to provide a reference for future research.The pretreatment techniques introduced liquid-liquid extraction (LLE),solid phase extraction (SPE),QuEChERS and salting out support liquid extraction,while the high-throughput detection technology included high-performance liquid chromatography (HPLC),liquid chromatography-mass spectrometry (LC-MS),ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS) and liquid chromatography-time of flight mass spectrometry (LC-TOF-MS).

Key words: veterinary drug residues; milk; high-throughput

CLC Number:

S879.1

LUO Runbo, ZHANG Yangdong, ZHENG Nan, WANG Jiaqi. Advances in High-throughput Detection of Veterinary Drug Residues in Milk and Dairy Products[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3410-3416.

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Advances in High-throughput Detection of Veterinary Drug Residues in Milk and Dairy Products

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