China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (1): 249-257.doi: 10.16431/j.cnki.1671-7236.2020.01.029

• Basic Veterinary Medicine • Previous Articles     Next Articles

Research Progress on Detection of Small Molecule Chemical Residues by Single-chain Antibody

HOU Ren1, HUANG Lingli1,2, TAO Yanfei1,2, XIE Changqing1,2, LIU Zhenli1,2, PENG Dapeng1,2   

  1. 1. National Reference Laboraory of Veterinary Drug Residues, The Key Laboratory for the Detection of Veterinary Drug Residues of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China;
    2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2019-05-23 Online:2020-01-20 Published:2020-01-17

Abstract: Excessive residues of small molecular chemical substances such as veterinary drugs,pesticides and biological toxins in food,feed or water cause a certain degree of harm to human and animal health and the balance of ecological environment.Therefore,it is necessary to establish a rapid detection method for the residue of small molecules.Immunoassay based on specific binding of antigen and antibody is a common method for residue detection.The key of this technique is to prepare antibodies that can be used to detect specific antigens.With the development of antibody structure analysis and recombinant DNA technology,single-chain antibody provide a new technique for the detection of small molecule chemical residues.Single-chain antibody has the advantages of small molecular weight,low immunogenicity,strong plasticity,and mass production,and have broad development space.In recent years,the detection of small molecule chemical residues by enzyme-linked immunosorbent assay based on single-chain antibody has become the most commonly used analytical tool.The single-chain antibody screening strategy,expression strategy,mutation strategy and fusion protein with alkaline phosphatase further improve antibody yield,sensitivity,broad spectrum and simplify the immunoassay method,thus promoting single-chain antibody immunoassay method for the detection of small molecule chemical residues.In this paper,recent advances on the application of single-chain antibody-based immunoassay methods in small molecule chemical substances such as veterinary drugs,pesticides and biotoxins are reviewed,aiming at providing reference for detection technology of small molecule chemical residue.

Key words: single-chain antibody; small molecule chemicals; residue detection

CLC Number: