单链抗体在小分子化学物质残留检测中的研究进展

doi:10.16431/j.cnki.1671-7236.2020.01.029

Abstract

Abstract: Excessive residues of small molecular chemical substances such as veterinary drugs,pesticides and biological toxins in food,feed or water cause a certain degree of harm to human and animal health and the balance of ecological environment.Therefore,it is necessary to establish a rapid detection method for the residue of small molecules.Immunoassay based on specific binding of antigen and antibody is a common method for residue detection.The key of this technique is to prepare antibodies that can be used to detect specific antigens.With the development of antibody structure analysis and recombinant DNA technology,single-chain antibody provide a new technique for the detection of small molecule chemical residues.Single-chain antibody has the advantages of small molecular weight,low immunogenicity,strong plasticity,and mass production,and have broad development space.In recent years,the detection of small molecule chemical residues by enzyme-linked immunosorbent assay based on single-chain antibody has become the most commonly used analytical tool.The single-chain antibody screening strategy,expression strategy,mutation strategy and fusion protein with alkaline phosphatase further improve antibody yield,sensitivity,broad spectrum and simplify the immunoassay method,thus promoting single-chain antibody immunoassay method for the detection of small molecule chemical residues.In this paper,recent advances on the application of single-chain antibody-based immunoassay methods in small molecule chemical substances such as veterinary drugs,pesticides and biotoxins are reviewed,aiming at providing reference for detection technology of small molecule chemical residue.

Key words: single-chain antibody; small molecule chemicals; residue detection

CLC Number:

S859.84

HOU Ren, HUANG Lingli, TAO Yanfei, XIE Changqing, LIU Zhenli, PENG Dapeng. Research Progress on Detection of Small Molecule Chemical Residues by Single-chain Antibody[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 249-257.

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Research Progress on Detection of Small Molecule Chemical Residues by Single-chain Antibody

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