三穗鸭SLC4A9基因表达量及其多态性对蛋壳品质的遗传效应分析

doi:10.16431/j.cnki.1671-7236.2021.06.021

Abstract

Abstract: In order to explore the genetic effects of solute carrier family 4 member 9 (SLC4A9) expression and polymorphism on the eggshell quality in duck,the expression levels of SLC4A9 gene in different tissues of Sansui ducks were detected by Real-time quantitative PCR,the SNP of SLC4A9 gene in Sansui ducks were directly sequenced by PCR products,and the genetic effect of SNP on the eggshell quality in Sansui ducks was analyzed.The results showed that the SLC4A9 gene mRNA was expressed to varying degrees in 10 tissues of Sansui ducks,with highly specific expression in heart and pancreas,moderate expression in kidney,and low expression in others.A total of 10 SNPs were detected among which three were located on exons and all were missense mutations(g.6803 C>T,g.7065 C>T and g.7089 C>G),and seven were located in introns (g.7162 C>G,g.11044 G>A,g.11090 T>C,g.11234 C>T,g.11261 C>T,g.11349 C>T and g.11403 G>A).Among them,g.6803 C>T mutation led to alanine (GCG) turning into valine (GTG),g.7065 C>T mutation caused alanine (GCC) to valine (GTC),g.7089 C>G mutation caused alanine (GCA) to glycine (GGA).χ² test found that g.6803 C>T,g.11090 T>C and g.11349 C>T deviated from Hardy-Weinberg equilibrium (P<0.05),and the genotype distribution of the other SNPs were in accordance with Hardy-Weinberg equilibrium (P>0.05).Correlation analysis results showed that the eggshell strength of CC and CT genotypes at g.11349 C>T was significantly higher than that of TT genotype (P<0.05).In conclusion,10 SNPs of SLC4A9 gene all affected the eggshell quality of Sansui ducks,and g.11349 C>T reached a significant level,which could provide reference for the improvement of eggshell quality and the selection of genetic markers in ducks.

Key words: Sansui ducks; SLC4A9 gene; organization expression; polymorphism; eggshell quality

CLC Number:

S834

YANG Suan, ZHANG Yiyu, TAN Yuancheng, WANG Yingtong, YOU Minfang, LIAO Chaomei. Genetic Effects Analysis of Polymorphism and Expression of SLC4A9 Gene on Eggshell Quality in Sansui Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2082-2090.

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Genetic Effects Analysis of Polymorphism and Expression of SLC4A9 Gene on Eggshell Quality in Sansui Ducks

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