屠宰场沙门氏菌耐药性分析和毒力基因检测

doi:10.16431/j.cnki.1671-7236.2022.10.027

Abstract

Abstract: 【Objective】 This study was aimed to investigate the drug resistance and virulence characteristics of Salmonella carried in slaughtered pork in Maoming,Guangdong province,and provide a basis for hazard assessment and formulation of prevention and control measures for foodborne Salmonella in this area.【Method】 Nineteen strains of Salmonella isolated from pork,spleen and liver samples collected from slaughterhouses in Maoming,Guangdong province were tested for resistance to β-lactams,fluoroquinolones,aminoglycosides,tetracyclines,aminoglycols and sulfonamides by the K-B drug-sensitive paper method,and the β-lactam resistance genes (bla_TEM,bla_OXA-1 and bla_SHV),fluoroquinolone resistance genes (qnrA,qnrS and qnrB),aminoglycoside resistance genes (aadA1,aac(6')-Ⅰb and rmtB),tetracycline resistance genes (tetA,tetB and tetC),aminol resistance genes (Cat1 and floR),sulfonamide resistance genes (SulⅠ,SulⅡ and SulⅢ) and 10 virulence genes (mogA,sseL,mgtC,bcfA,araB,spvR,spvA,spvB,spvC and spvD) were detected by PCR method.【Result】 19 strains of Salmonella were highly resistant to tetracycline,doxycycline,chloramphenicol,florfenicol and sulfaisoxazole,and the resistance rates were more than 50%,with the highest resistance rates of 89.5% (17/19) to tetracycline,doxycycline and chloramphenicol,and 89.5% to 3 or more antimicrobial drugs,with the highest resistance to all 11 antimicrobial drugs.The detection rate of bla_TEM,qnrA,aadA1, aac(6')-Ⅰb,Cat1,floR,SulⅠ and SulⅢ resistance genes was high (≥50.0%),the highest detection rate of bla_TEM resistance gene was 84.2%,73.7% of strains carried 5 or more resistance genes at the same time,and the highest strain carried 12 drug-resistant genes;mogA,mgtC,bcfA,araB virulence genes were all detected and the detection rate was above 70%,of which mogA virulence gene had a detection rate of up to 100%,and the remaining 6 virulence genes did not detect out.【Conclusion】 Salmonella from the pork of Maoming slaughterhouse in Guangdong province was seriously multidrug-resistance,carrying multiple drug-resistance genes with complex gene combinations and multiple virulence genes.

Key words: slaughterhouse; Salmonella; drug resistance; resistance gene; virulence gene

CLC Number:

S852.61

JIN Haozhan, LI Liang, WEI Qilin, HAN Yixin, XU Zhihong, KANG Huahua, XIANG Rong. Drug Resistance Analysis and Virulence Gene Detection of Salmonella in Slaughterhouses[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3943-3952.

References

[1] MAJOWICZ S E,MUSTO J,SCALLAN E,et al.The global burden of nontyphoidal Salmonella gastroenteritis[J].Clinical Infectious Diseases,2010,50(6):882-889.
[2] LI Q,YIN J,LI Z,et al.Serotype distribution,antimicrobial susceptibility,antimicrobial resistance genes and virulence genes of Salmonella isolated from a pig slaughterhouse in Yangzhou,China[J]. AMB Express,2019,9(1):210.
[3] 王丽,石晓丽,杨桂娥.非预包装食品中沙门氏菌快速检测方法的建立[J].食品研究与开发,2020,41(23):179-184. WANG L,SHI X L,YANG G E.Establishment of rapid detection method for Salmonella in non-prepackaged foods[J].Food Research and Development,2020,41(23):179-184.(in Chinese)
[4] JU TEH C S,LAU M Y,CHONG C W,et al.One-step differential detection of Salmonella enterica serovar Typhi,serovar Paratyphi A and other Salmonella spp.by using a quadruplex Real-time PCR assay[J].Journal of Microbiological Methods,2021,183:106184.
[5] 陈俊,蒋文灿,谭天,等.沙门氏菌毒力岛及Ⅲ型分泌系统研究进展[J].中国人兽共患病学报,2015,31(4):371-376. CHEN J,JIANG W C,TAN T,et al.Salmonella pathogenicity island and type Ⅲ secretion system[J].Chinese Journal of Zoonoses,2015,31(4):371-376.(in Chinese)
[6] NADI Z R,SALEHI T Z,TAMAI I A,et al.Evaluation of antibiotic resistance and prevalence of common Salmonella enterica serovars isolated from foodborne outbreaks[J].Microchemical Journal,2020,155:104660.
[7] PARISI A,CRUMP J A,GLASS K,et al.Health outcomes from multidrug-resistant Salmonella infections in high-income countries:A systematic review and meta-analysis[J].Foodborne Pathogens and Disease,2018,15(7):428-436.
[8] YANG J,JU Z J,ZHAO X N,et al.Serotype,antimicrobial susceptibility and genotype profiles of Salmonella isolated from duck farms and a slaughterhouse in Shandong province,China[J].BMC Microbiology,2019,19(1):202.
[9] 李月华,赵格,赵建梅,等.欧盟、美国及国内畜禽屠宰环节沙门氏菌监控现状[J].中国动物检疫,2021,38(6):69-75. LI Y H,ZHAO G,ZHAO J M,et al.Discussion on the monitoring status of Salmonella during livestock and poultry slaughtering in EU,the United State and China[J].China Animal Quarantine,2021,38(6):69-75.(in Chinese)
[10] 方忠意,李金磊,董鹏,等.河南省猪源沙门氏菌分离鉴定、血清分型及药物敏感性分析[J].中国动物检疫,2019,36(4):74-78. FANG Z Y,LI J L,DONG P,et al.Isolation,identification and serotyping of swine Salmonella in Henan province and analysis on its drug sensitivity[J].China Animal Quarantine,2019,36(4):74-78.(in Chinese)
[11] 韩依辛,田雅,马广宇,等.广东10个屠宰场猪肉中3种食源性致病菌的检测[J].动物医学进展,2021,42(2):50-54. HAN Y X,TIAN Y,MA G Y,et al.Detection of three foodborne pathogens in pork from 10 slaughterhouses in Guangdong[J].Progress in Veterinary Medicine,2021,42(2):50-54.(in Chinese)
[12] 贺恒旭,刘璐函,刘俊峰,等.生猪屠宰环节沙门氏菌携带状况、耐药性及其耐药基因分析[J].食品安全质量检测学报,2021,12(8):3034-3040. HE H X,LIU L H,LIU J F,et al.Prevalence,antimicrobial resistance phenotypes and antimicrobial resistance genes of Salmonella isolates from pig slaughtering[J].Journal of Food Safety and Quality,2021,12(8):3034-3040.(in Chinese)
[13] ABRAHAM R,SAHIBZADA S,JORDAN D,et al.Antimicrobial resistance and genomic relationships of Salmonella enterica from Australian cattle[J].International Journal of Food Microbiology,2022,371:109672.
[14] 吴荔琴,刘佩怡,伍宏凯,等.2014~2016年广州市屠宰场分离沙门氏菌血清学鉴定和耐药性分析[J].中国兽药杂志,2017,51(7):15-20. WU L Q,LIU P Y,WU H K,et al.Identification serotype and antimicrobial resistance of Salmonella from the slaughter houses in Guangzhou from 2014 to 2016[J].Chinese Journal of Veterinary Drug,2017,51(7):15-20.(in Chinese)
[15] 贾爱卿.猪沙门氏菌的临床分离鉴定及耐药性消除研究[D].武汉:华中农业大学,2006. JIA A Q.Isolation of Salmonella from diseased pigs and the reversal of multidrug resistance for Salmonella Typhimurium[D].Wuhan:Huazhong Agricultural University,2006.(in Chinese)
[16] 邓珊珊,张海,蔺俐仲,等.规模化猪场猪大肠杆菌的耐药性及相关耐药基因检测[J].养猪,2021,1:113-115. DENG S S,ZHANG H,LIN L Z,et al.Drug resistance and related gene retection of Escherichia coli in large-scale pig farms[J]. Swine Production,2021,1:113-115.(in Chinese)
[17] 宋艳.山东省部分种鸡场死胚中沙门氏菌的鉴定及耐药特性研究[D]. 泰安:山东农业大学,2020. SONG Y.Identification of Salmonella in dead embryos and study on their drug resistance characteristics of some farms in Shandong province[D].Tai’an:Shandong Agricultural University,2020.(in Chinese)
[18] 袁佳慧.耐药大肠埃希菌β-内酰胺类、氨基糖苷类耐药基因及可移动遗传元件研究分析[D].南京:南京医科大学,2019. YUAN J H.Analysis of drug resistance genes and mobile genetic elements in drug-resistant Escherichia coli[D].Nanjing:Nanjing Medical University,2019.(in Chinese)
[19] 唐一鸣.猪源大肠杆菌和沙门氏菌耐药表型与基因型的研究[D].杭州:浙江大学,2010. TANG Y M.Phenotypic and genotypic characterization of antimicrobial resistance in porcine pathogenic Escherichia coli and Salmonella[D].Hangzhou:Zhejiang University,2010.(in Chinese)
[20] 宋雪,赵格,刘文化,等.不同来源沙门氏菌的毒力基因检测与耐药性分析[J].中国动物检疫,2017,34(5):40-46. SONG X,ZHAO G,LIU W H,et al.Comparative analysis of virulent genes and drug resistance of Salmonella isolated from different sources[J].China Animal Quarantine,2017,34(5):40-46.(in Chinese)
[21] M100-S23.Performance standards for antimicrobial susceptibility testing:Twenty-third informational supplement[S].Wayne:CLSI,2013.
[22] 钟巧贤,袁淑英,梁秋燕,等.屠宰场分离大肠埃希氏菌O157∶H7药敏试验及耐药基因分析[J].动物医学进展,2021,42(6):140-144. ZHONG Q X,YUAN S Y,LIANG Q Y,et al.Drug sensitivity test and drug resistance gene analysis of Escherchia coli O157∶H7 isolated from slaughterhouse [J].Progress in Veterinary Medicine,2021,42(6):140-144.(in Chinese)
[23] LI X Z,MCHROTRA M,GHINIRE S,et al.Beta-lactam resistance and beta-lactamases in bacteria of animal origin[J].Veterinary Microbiology,2007,121(3-4):197-214.
[24] 刘艳红.沙门氏菌和大肠杆菌耐药性及耐药基因的研究[D].沈阳:沈阳农业大学,2016. LIU Y H.Research of drug resistance and resistance genes of Salmonella and Escherichia coli[D].Shenyang:Shenyang Agricultural University,2016.(in Chinese)
[25] 张海,方英,徐春志,等.规模化猪场猪源沙门氏菌的耐药性及相关耐药基因检测[J].养猪,2019,3:105-107. ZHANG H,FANG Y,XU C Z,et al.Detection of drug resistance and related drug resistance genes of Salmonella Typhimurium in large-scale pig farms[J].Swine Production,2019,3:105-107.(in Chinese)
[26] 段合波.食源性沙门氏菌流行病学、耐药特征及全基因组测序分析[D].武汉:武汉轻工大学,2020. DUAN H B.Epidemiology,antimicrobial resistance characteristics and whole genome sequencing analysis of food-borne Salmonella[D].Wuhan:Wuhan Polytechnic University,2020.
[27] MENG X,MENG X C,ZHU C H,et al.The RNA chaperone Hfq regulates expression of fimbrial-related genes and virulence of Salmonella enterica serovar Enteritidis[J].FEMS Microbiology Letters,2013,346(2):90-96.
[28] 王鑫盛.生猪屠宰过程中主要食源性致病菌检测及沙门氏菌耐药性与毒力基因分析[D].郑州:河南农业大学,2021. WANG X S.Detection of mian foodborne pathogens during pig slaughter and analysis of Salmonella resistance and virulence genes[D].Zhengzhou:Henan Agricultural University,2021.(in Chinese)
[29] 黄秀梅,张倩,盖文燕,等.山东地区屠宰场猪肉污染沙门氏菌菌株毒力基因筛查与ERIC-PCR分型[J].中国人兽共患病学报,2018,34(8):697-702. HUANG X M,ZHANG Q,GAI W Y,et al.Genetic screening and ERIC-PCR genotyping of Salmonella from pork in slaughterhouses in Shandong province,China[J].Chinese Journal of Zoonoses,2018,34(8):697-702.(in Chinese)

Drug Resistance Analysis and Virulence Gene Detection of Salmonella in Slaughterhouses

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