›› 2018, Vol. 45 ›› Issue (3): 822-829.doi: 10.16431/j.cnki.1671-7236.2018.03.033

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Isolation, Identification and Drug Resistance Analysis of Streptococcus suis Serotype 2 in Tianjin

YANG Chunlei1,2, DONG Zhimin1,2, LI Xiuli1,2, YAN Xiaocui1,2, TIAN Xiangxue1,2, CHI Jingjing1,2, REN Weike1,2, ZHANG Lei1,2, YAN Minghua1,2   

  1. 1. Tianjin Animal Husbandry and Veterinary Research Institute, Tianjin 300381, China;
    2. Tianjin Engineering Research Center for Livestock and Poultry Health Breeding, Tianjin 300381, China
  • Received:2017-08-30 Online:2018-03-20 Published:2018-03-22

Abstract:

In order to investigate the prevalence and drug resistance of Streptococcus suis serotype 2 (SS2) in Tianjin,317 samples of suspected Streptococcus suis disease were collected from large-scale pig farms in this area.11 SS2 strains from the samples were identified by cultural character,dyeing property analysis,biochemical test and PCR amplification.The pathogenicity and drug susceptibility test of the isolates were also carried out.The pathogenicity test result showed that 6 strains were lethal to mice in the 11 SS2 isolate strains,including 1 strain with high lethality and 5 strains with low lethality.Drug sensitivity test result indicated the 11 isolates were highly resistant to clinical drugs,the resistance rate was up to 100.00% to amikacin,tetracycline and doxycycline.All of the 11 isolates were multidrug resistant,and 4 SS2 isolates were resistant to 9 drugs among them,which was accounting for 36.36%(4/11).The results showed that SS2 occurred in Tianjin area and the pathogenic bacteria were resistant to many antibacterials.Farmers should paid close attention to this,rational,scientific,standardized medication and alternative medication when prevented the Streptococcus suis disease.

Key words: Streptococcus suis serotype 2; isolation and identification; drug resistance; Tianjin

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