乳酸菌发酵物与中药配伍对预防鸡大肠杆菌病协同作用的研究

doi:10.16431/j.cnki.1671-7236.2015.07.039

Abstract

Abstract: The experiment was aimed to detect the effect of lactic acid bacteria yeast matching with traditional Chinese medicine (TCM) on efficacy of TCM preventing chicks from colibacillosis.The CTCML compound used in the experiment was composed of effective TCM formula to chick colibacillosis and lactic acid bacteria yeast.Chicks were artificially infected with E.coli for disease model by injecting 0.2 mL (9×10⁸ CFU/mL) E.coli culture into breast muscles.144 eight-day-old chicks were divided into 9 groups,in which CTCML high-dose,middle-dose and low-dose groups were treated with CTCML particles at the dose of 0.6,0.4 and 0.2 g/d respectively before chicks being infected,and mixing the extract liquid of CTCML with drink at the dose of 0.3,0.2 and 0.1 mL/d respectively after chicks being infected.Three TCM high-dose,middle-dose and low-dose groups were treated with TCM at the same doses as CTCML groups.The drug control group was treated with ciprofloxacin hydrochloride.Meanwhile,the infection control group and normal control group were set up.The survival rates of CTCML high-dose,middle-dose and low-dose groups,TCM high-dose,middle-dose and low-dose groups,drug control group,infection control group and normal control group were 75.00%,75.00%,93.75%,68.75%,68.75%,75.00%,75.00%,56.25% and 100.00%,respectively.There were significant differences between CTCML low-dose group and CTCML high-dose and middle-dose groups,TCM high-dose,middle-dose and low-dose groups,drug control group,infection control group (P<0.05).But there were no significant differences between CTCML high-dose and middle-dose groups,TCM high-dose,middle-dose,low-dose groups and drug control group each other (P>0.05).The rate of weight gain of CTCML low-dose group was lower by 29.93% than that of normal control group.CTCML low-dose group had the lowest effect on weight gain of chicks in all infection groups.Compared to normal control group,the rate of weight gain of CTCML middle-dose group reduced by 36.95%,the rate of weight gain of other infection groups reduced by 42.49% to 51.07%.The results showed that TCM was effective on protecting chicks from E.coli infection and the lactic acid bacteria had obvious synergistic effect on TCM.

Key words: traditional Chinese medicine; lactic acid bacteria; compound; synergistic effect; chicks; E.coli; artificial infection; survival rate

CLC Number:

S853.7

WANG Jian-guo, LI Xiu-li, FAN Huan, CHI Jing-jing, TIAN Xiang-xue, LI Fu-qiang, MENG Fan-rui, SHI Hui-ling, WANG Wen-jie. Study on Synergistic Effect of Lactic Acid Bacteria Yeast Matched with Traditional Chinese Medicine on Preventing Chicks from Colibacillosis[J]. , 2015, 42(7): 1890-1896.

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Study on Synergistic Effect of Lactic Acid Bacteria Yeast Matched with Traditional Chinese Medicine on Preventing Chicks from Colibacillosis

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