马身猪耳缘组织成纤维细胞的体外培养与鉴定

doi:10.16431/j.cnki.1671-7236.2017.08.011

Abstract

Abstract:

In order to study the biological characteristics of somatic cells of Mashen pig, the ear marginal fibroblast cell lines of Mashen pig were established from 3 days old Mashen pig by direct explant method,and the biological characteristics of cell lines were detected. The results showed that the cell lines obtained from Mashen pig were in conformity with basic properties of typical fibroblasts, the shape of adherent cells were typical spindle, star and polygon, the time of grew into monolayer cells was 10 to 13 d, the growth curve was typical S shaped. The frequency of metaphase chromosome number (2n=38) was about 84% of the total number of cells, and it had met the establishment standard of fibroblast cell line. The transfection efficiency of green fluorescent protein(pEGFP-C1)transfected by lipofectamine-mediated method was 25%. The successful establishment of the fibroblast cell lines of Mashen pig had opened up a new method for the conservation of genetic resources.

Key words: Mashen pig; fibroblast cell lines; explant culture

CLC Number:

Q813.1⁺1

ZHANG Qi, GUO Xiao-hong, GAO Peng-fei, JIN Yu-shu, LI Meng, CHENG Zhi-min, ZHANG Ning-fang, LE Bao-yu, LIU Hong, CAO Guo-qing, LI Bu-gao. In vitro Culture and Identification of Ear Marginal Fibroblast Cell Lines Derived from Mashen Pigs[J]. , 2017, 44(8): 2289-2294.

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In vitro Culture and Identification of Ear Marginal Fibroblast Cell Lines Derived from Mashen Pigs

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