番鸭ERBB4基因组织表达、克隆及序列分析

doi:10.16431/j.cnki.1671-7236.2020.06.001

Abstract

Abstract: This study was aimed to detect the relative expression level of receptor tyrosine protein kinase 4 (ERBB4) gene in brain under different physiological conditions of muscovy ducks,analyze its bioinformatics function,and explore whether ERBB4 gene affected the aggressive behavior of ducks.The relative expression of ERBB4 gene in striatum and hypothalamus of muscovy ducks was detected by Real-time quantitative PCR.The CDS core region of ERBB4 gene was cloned and sequenced by RT-PCR,analyze the homology and genetic evolution of ERBB4 gene,and predict the physicochemical properties,subcellular localization,signal peptide,secondary structure and tertiary structure by bioinformatics analysis tools.The results showed that ERBB4 gene was expressed in both brain tissues of muscovy ducks,the expression in brain of muscovy ducks with aggressive behavior was higher than that of normal group,and the expression in striatum was slightly higher than that of hypothalamus.The homology of nucleotide and amino acid sequences showed that ERBB4 gene had certain genetic diversity among different species and was closely related to Anas platyrhynchos.ERBB4 protein was hydrophilic and stable.There was no signal peptide at the N-terminal of the amino acid sequence,5 furan-like cysteine (FU) rich domains and 1 tyrosine kinase (TyrKc) protein domain.The subcellular localization was in cytoplasm,and the spatial structure was mainly right-handed alpha helix and random coil.In addition,ERBB4 gene might play an important role in the nervous system of muscovy ducks,this results provided a reference for further exploring the mechanism of ERBB4 gene in aggressive behavior of muscovy ducks.

Key words: muscovy ducks; ERBB4 gene; cloning; organizational expression; bioinformatics analysis

CLC Number:

ZHOU Xuan, YANG Shenglin, YANG Shihao, LUO Linli, ZHU Yongcai, ZHANG Yun. Tissue Expression,Cloning and Bioinformatics Analysis of ERBB4 Gene in Muscovy Duck (Cairna moschata)[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1637-1647.

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Tissue Expression,Cloning and Bioinformatics Analysis of ERBB4 Gene in Muscovy Duck (Cairna moschata)

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