1株信鸽源鼠伤寒沙门氏菌的分离鉴定及其耐药性和毒力分析

doi:10.16431/j.cnki.1671-7236.2021.01.037

Abstract

Abstract: The purpose of this research was to identify the etiology of dead pigeons which clinically suspected Salmonella infection,and to analyze the drug resistance and virulence of pathogenic bacteria.A series of tests including bacterial isolation and culture,colonial morphology observation,staining microscopy,biochemical identification,serotype analysis,16S rRNA gene sequencing analysis,serum plate agglutination test of rehabilitated racing pigeons were used for indentification,drug sensitivity test,drug resistance genes and virulence genes detection were carried out to analyze drug resistance and virulence.The results showed that the isolated bacteria were black colonies on the BS,XLD and HE medium,and the bacteria were Gram-negative short-bacillus without capsule and spores under microscope observation,the biochemical reaction results of the isolated strain conformed to the biochemical characteristics of Salmonella,the serotype of the isolated strain was 1,4,12:i:1,2.The phylogenetic tree analysis of the 16S rRNA gene sequence showed that the isolated bacterial strain and Salmonella Typhimurium were clustered into one branch with the homology >99%.Specific agglutination occurred between the serum of rehabilitated racing pigeons and isolated strain,therefore the isolated bacterial strain was identified as Salmonella Typhimurium considering the results of biochemical identification and serotype detection.The results of drug sensitivity test and drug resistance gene test showed that the isolated bacterial strain was resistant to florfenicol,the floR and cmlA genes of chloramphenicol resistance in isolated bacterial strain were detected which was consistent with the drug resistance phenotype.The isolated bacterial strain was tested positive for 17 virulence genes including mogA and so on.In this study,one strain of Salmonella Typhimurium was successfully isolated and identified from racing pigeons,which was resistant to florfenicol and had highly virulence,providing reference for the prevention and treatment of Salmonella infection in racing pigeons in the next work.

Key words: racing pigeon; Salmonella Typhimurium; isolation; identification; drug resistance; virulence

CLC Number:

S858.39

ZHANG Qilong, LI Yunpeng, FU Caixia, LIU Haiying, ZHANG Wei, WANG Lin, WU Di, LIU Xiaodong, ZHOU Degang. Isolation,Identification,Drug Resistance and Virulence Analysis of one Salmonella Typhimurium Strain from Racing Pigeons[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 338-347.

References

[1] ENG S K,PUSPAPAJAH P,AB MUTALIB N S,et al.Salmonella:A review on pathogenesis,epidemiology and antibiotic resistance[J].Frontiers in Life Science,2015,8(3):284-293.
[2] AJMERA A,SHABBIR N.Salmonella[M].Treasure Island (FL):StatPearls Publishing,2020.
[3] WANG M Y,QAZI I H,WANG L L,et al.Salmonella virulence and immune escape[J].Microor-ganisms,2020,8(3):407.
[4] BUMANN D,SCHOTHORST J.Intracellular Salmonella metabolism[J].Cellular Microbiology,2017,19(10):e12766.
[5] BLAIR J M A,WEBBER M A,BAYLAY A J,et al.Molecular mechanisms of antibiotic resistance[J].Nature Reviews Microbiology,2015,13(1):42.
[6] 郑林,祝令伟,郭学军,等. 沙门氏菌主要流行血清型耐药性的研究进展[J].江苏农业科学,2020,48(6):8-12. ZHENG L,ZHU L W,GUO X J,et al.Advances in the study of drug resistance of major epidemic serotypes of Salmonella[J].Jiangsu Agricultural Sciences,2020,48(6):8-12.(in Chinese)
[7] 史玉颖,刘玉山,齐霖,等.浅谈赛鸽沙门氏菌病[J].家禽科学,2019,12:36-38. SHI Y Y,LIU Y S,QI L,et al.A brief discussion on racing pigeon Salmonellosis[J].Poultry Science,2019,12:36-38.(in Chinese)
[8] XIE B,DUPRAS A A,DUCEPPE M O,et al.Genome sequences of 13 isolates of Salmonella enterica serovar Typhimurium var.copenhagen obtained from wild pigeons in Canada[J].Genome Announcements,2018,6(20):e00392-18.
[9] 赵巧雅,刘娜,潘华,等.2株鸽源沙门氏菌病原的分离鉴定及诊治分析[J].家禽科学,2020,2:17-21. ZHAO Q Y,LIU N,PAN H,et al.Isolation,identification,diagnosis and treatment of two Salmonella strains isolated from pigeon[J].Poultry Science,2020,2:17-21.(in Chinese)
[10] TORRES-MEJÍA A M,BLANCO-PEA K,RODRÍGUEZ C,et al.Zoonotic agents in feral pigeons (Columba livia) from Costa Rica:Possible improvements to diminish contagion risks[J].Vector Borne and Zoonotic Diseases,2018,18(1):49-54.
[11] SEO K W,KIM J J,MO I P,et al.Molecular characteristic of antimicrobial resistance of Salmonella Gallinarum isolates from chickens in Korea,2014 to 2018[J].Poultry Science,2019,98(11):5416-5423.
[12] 陈国权,张旭,阎朝华,等.鸭源多杀性巴氏杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2020,47(2):620-628. CHEN G Q,ZHANG X,YAN Z H,et al.Isolation,identification and drug resistance analysis of Pasteurella multocida from ducks[J].China Animal Husbandry & Veterinary Medicine,2020,47(2):620-628.(in Chinese)
[13] AJAYI A,SMITH S I,KALPY J C,et al.Molecular diversity and antibiotic resistance gene profile of Salmonella enterica serovars isolated from humans and food animals in Lagos,Nigeria[J].Acta Microbio-logica et Immunologica Hungarica,2019,66(4):509-527.
[14] HAI D,YIN X P,LU Z X,et al.Occurrence,drug resistance,and virulence genes of Salmonella isolated from chicken and eggs[J].Food Control,2020,113:107109.
[15] 黄秀梅,张倩,盖文燕,等.山东地区屠宰场猪肉污染沙门氏菌菌株毒力基因筛查与ERIC-PCR分型[J].中国人兽共患病学报,2018,34(8):697-702. HUANG X M,ZHANG Q,GAI W Y,et al.Genetic screening and ERIC-PCR genotyping of Salmonella from pork in slaughter houses in Shandong province,China[J].Chinese Journal of Zoonoses,2018,34(8):697-702.(in Chinese)
[16] TESKE L,RYLL M,RUBBENSTROTH D,et al.Epidemiological investigations on the possible risk of distribution of zoonotic bacteria through apparently healthy homing pigeons[J].Avian Pathology,2013,42(5):397-407.
[17] 张跃东,罗薇,葛润洲,等.四川部分区域鸽源沙门菌携带情况的调查及其部分生物学特性的分析[J].中国兽医科学,2019,49(3):324-332. ZHANG Y D,LUO W,GE R Z,et al.Investigation of situation of pigeon-origin Salmonella and analysis of some of biological characteristics of Salmonella in pigeons in part of Sichuan province[J].Chinese Veterinary Science,2019,49(3):324-332.(in Chinese)
[18] 罗青平,温国元,王红琳,等.中国鸽病的流行及防控现状[J].湖北农业科学,2015,54(12):2954-2956. LUO Q P,WEN G Y,WANG H L,et al.The epidemic situation of pigeon disease and its prevention and control in China[J].Hubei Agricultual Sciences,2015,54(12):2954-2956.(in Chinese)
[19] MOORE C E.Changes in antibiotic resistance in animals[J].Science,2019,365(6459):1251-1252.
[20] VAN BOECKEL T P,PIRES J,SILVESTER R,et al.Global trends in antimicrobial resistance in animals in low-and middle-income countries[J].Science,2019,365(6459):eaaw1944.
[21] ARZANLOU M,CHAI W C,VENTER H.Intrinsic,adaptive and acquired antimicrobial resistance in Gram-negative bacteria[J].Essays in Biochemistry,2017,61(1):49-59.
[22] 程旭,沈欣悦,刘梅,等.鸽源鼠伤寒沙门菌分离鉴定及药敏试验[J].中国兽医杂志,2016,52(8):99-100. CHENG X,SHEN X Y,LIU M,et al.Isolation,identification and drug susceptibility test of Salmonella Typhimurium from pigeon[J].Chinese Journal of Veterinary Medicine,2016,52(8):99-100.(in Chinese)
[23] 吕敏娜,孙铭飞,沈浩铎.2011~2018年广东鸽源大肠杆菌、沙门氏菌耐药性分析[J].畜牧兽医科技信息,2019,12:46-48. LYU M N,SUN M F,SHEN H D.Analysis on the drug resistance of E.coli and Salmonella from pigeon from 2011 to 2018 in Guangdong[J].Chinese Journal of Animal Husbandry and Veterinary Medicine,2019,12:46-48.(in Chinese)
[24] 周荣云,李军朝,邸涛,等.21株禽源鼠伤寒沙门氏菌的分离鉴定及耐药性研究[J].黑龙江畜牧兽医,2016(16):131-133. ZHOU R Y,LI J C,DI T,et al.Isolation and identification of 21 strains of Salmonella Typhimurium from poultry and study on drug resistance[J].Heilongjiang Animal Science and Veterinary Medicine,2016(16):131-133.(in Chinese)
[25] FABREGA A,VILA J.Salmonella enterica serovar Typhimurium skills to succeed in the host:Virulence and regulation[J].Clinical Microbiology Reviews,2013,26(2):308-341.
[26] JOHNSON R,MYLONA E,FRANKEL G.Typhoidal Salmonella:Distinctive virulence factors and pathogenesis[J].Cellular Microbiology, 2018,20(9):e12939.
[27] MOEST T P,MÉRESSE S.Salmonella T3SSs:Successful mission of the secret(ion) agents[J].Current Opinion in Microbiology,2013,16(1):38-44.
[28] AZIMI T,ZAMIRNASTA M,SANI M A,et al.Molecular mechanisms of Salmonella effector proteins:A comprehensive review[J].Infection and Drug Resistance,2020,13:11-26.
[29] 孙阳阳,杨美,解晓莉,等.驴源沙门氏菌的分离鉴定、耐药及毒力分析[J].山东农业科学,2019,51(11):143-148. SUN Y Y,YANG M,XIE X L,et al.Isolation,identification,drug resistance and virulence analysis of Salmonella from donkey[J].Shandong Agricultural Sciences,2019,51(11):143-148.(in Chinese)
[30] KARACAN SEVER N,MEHMET A.Molecular analysis of virulence genes of Salmonella infantis isolated from chickens and turkeys[J].Microbial Pathogenesis, 2019,126:199-204.
[31] 宫艳杉.山东地区死亡鸭胚中细菌分离鉴定及沙门氏菌耐药性与毒力基因检测[D].泰安:山东农业大学,2017. GONG Y S.Isolation,identification and research of resistance genes and virulence genes of Salmonella from dead duck embryos in Shandong province[D].Tai'an:Shandong Agricultural University,2017.(in Chinese)
[32] DETWEILER C S,MONACK D M,BRODSKY I E,et al.VirK,somA and rcsC are important for systemic Salmonella enterica serovar Typhimurium infection and cationic peptide resistance[J].Molecular Micro-biology,2003,48(2):385-400.
[33] DOS SANTOS A M P,FERRARI R G,CONTE-JUNIOR C A.Virulence factors in Salmonella Typhimurium:The sagacity of a bacterium[J].Current Microbiology,2019,76(6):762-773.
[34] 王德宁.鸡源沙门氏菌耐药性、致病性与毒力基因相关性分析[D].哈尔滨:东北农业大学,2014. WANG D N.Correlation analysis among drug-resistance,pathogenicity and virulence genes of Salmonella isolated from chickens[D].Harbin:Northeast Agricultural University,2014.(in Chinese)
[35] 冯丽娜,李从荣,姜树朋,等.产NDM ExPEC毒力与其耐药性和分子分型的关系研究[J].国际检验医学杂志,2020,41(8):910-915. FENG L N,LI C R,JIANG S P,et al.The relationship between virulence factors and molecular epidemiology and carbapenem-resistance of New Delhi metallo-beta-lactamase-producing extraintestinal patho-genic Escherichia coli[J].International Journal of Laboratory Medicine,2020,41(8):910-915.(in Chinese)
[36] AMMAR A M,ABDEEN E E,ABO-SHAMA U H,et al.Molecular characterization of virulence and antibiotic resistance genes among Salmonella serovars isolated from broilers in Egypt[J].Letters in Applied Microbiology.2019,68(2):188-195.

Isolation,Identification,Drug Resistance and Virulence Analysis of one Salmonella Typhimurium Strain from Racing Pigeons

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yangyang, QIN Shizhen, TANG Defu, SHAO Yuxin, SHI Zhaoguo, WANG Zheng, LI Xing, ZHAO Dongdong. Effects of Zinc, Lactobacillus on Immune and Antioxidant Function of Squabs Infected with Salmonella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2276-2285.
[2]	SONG Yue, SU Shengjie, ZHANG Fan, BAI Fan, DAI Lingli, WANG Na, WANG Dawei, YANG Qianwen, ZHAO Shihua, ZHANG Yuemei. Prokaryotic Expression and Polyclonal Antibody Preparation of Mannheimia haemolytica Truncated Leukotoxin [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2479-2486.
[3]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.
[4]	LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.
[5]	CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.
[6]	PENG Yangxiang, YANG Zhenbiao, YAN Kuiyou, WANG Han, HU De, WANG Zun, LIU Ning, ZHAO Liansheng. From Artificial to Intelligent:Research Progress of Individual Idendification Technology for Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1855-1866.
[7]	ZENG Chengrong, LIU Xin, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, WEN Ming, ZHOU Bijun, CHEN Jiangfeng, JIANG Haibo. Research Progress on Quorum Sensing System of Aeromonas hydrophila and Its Inhibitor Application [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1991-2000.
[8]	LIU Caixia, SHI Weidi, KOU Lijun, MA Xun, WANG Jing, GAO Shengjie, LYU Shuangfei, CHEN Xuke, ZENG Dongdong, KONG Cuilian, REN Huijie. Regulation of Transcription of Key Virulence Factors of Listeria monocytogenes by LIPI-4 EⅡC [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1340-1351.
[9]	LI Zhijie, ZHAO Ying, MA Xin, WANG Meng, YU Sijiu, WANG Libin, PAN Yangyang. Research Progress on Isolation Methods and Main Reproductive Functions of Exosomes from Animal Follicular Fluid [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1480-1488.
[10]	TIAN Changqing, ZHANG Kunzhong, QI Yumei, SHI Wenjing, DONG Zhijie, ZHANG Haohao, HE Zengwen, ZHI Ji, ZHAO Xuehui, CHONG Qian, XUE Huiwen, GOU Huitian. Isolation,Identification and Biological Characteristics Analysis of a Strain of Listeria monocytogenes from Environment [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1543-1555.
[11]	WANG Lucai, SALANG Wenzhu, ZHANG Huanrong. Isolation,Identification and Drug Resistance and Pathogenicity Analysis of Salmonella Enteritidis from Avian [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1663-1674.
[12]	WU Zhi, ZHANG Cong, YUAN Huisha, DONG Hongyan, DAI Lixia, JIA Luqi, WU Shuang, ZHU Shanyuan. Isolation,Identification,Virulence and Drug Resistance of Salmonella from Yellow Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1695-1706.
[13]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[14]	ZHU Qiqi, ZHANG Jiyu, WANG Chengye, WU Lingyu, WANG Weiwei, ZHU Zhen. Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1081-1092.
[15]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.