马乳脂肪球膜蛋白组鉴定及潜在功能分析

doi:10.16431/j.cnki.1671-7236.2021.11.012

Abstract

Abstract: The purpose of this study was to investigate the protein composition and potential biological function of milk fat globule membrane (MFGM) in horses. Twelve healthy thoroughbred mares with 5 years old and body weight of 450-500 kg were selected as experimental animals, they were mainly fed with hay. The feeding-and-management conditions of all experimental horses were the similarity. 12 samples were mixed with 4 samples in each group, and MFGM proteins were isolated and extracted from horse milk on the 60th day after delivery. Of these, 4 individual sample was mixed and 3 biological samples were prepared. The tryptic peptides of samples were identified by high-resolution mass spectrometry and bioinformatics analysis. The results showed that 310 proteins were identified in MFGM fraction of horses, the identified MFGM proteins were mainly involved in biological regulation, stimulus response, localization, multicellular biological processes, transportation, signaling, cell communication, developmental processes, cell differentiation and immune system process on the basis of biological processes. They were mainly located in the extracellular area, membrane, vesicle, nucleolus and mitochondria according to cellular components. They were mainly involved in catalytic activity, protein binding, carbohydrate derivative binding and small molecule binding regarding of molecular functions. KEGG pathway analysis of MFGM proteins indicated that several proteins were involved in platelet activation pathway, endocytosis, fatty acid biosynthesis and Ras signaling pathways. The protein-protein interaction network analysis of MFGM protein in horses contained 215 proteins. The results of this study revealed the complexity of MFGM proteome in horses, and provided scientific data for analyzing its nutritional and biological functions.

Key words: horse milk; milk fat globule membrane protein; biological function; bioinformatics analysis

CLC Number:

TQ937

CHANG Huaxiang, LI Xiaobin, XIONG Suyu, ZHANG Junyu, CHEN Kaixu, LIU Jiancheng, LI Ning, ZANG Changjiang, LI Fengming. Proteome Identification and Potential Function Analysis of Milk Fat Globule Membrane in Horses[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4025-4034.

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Proteome Identification and Potential Function Analysis of Milk Fat Globule Membrane in Horses

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