谷氨酰胺替代部分甘油对冻融猪精子获能和体外受精能力的影响

doi:10.16431/j.cnki.1671-7236.2021.11.022

Abstract

Abstract: The purpose of this experiment was to explore the effect of glutamine (Gln) instead of partial glycerol on capacitation and in vitro fertilization ability of frozen-thawed pig sperm. The experiment was divided into 6 groups:3% glycerol control group and 5 treatment groups (groups Ⅰ-Ⅴ:2% glycerol+glutamine (0, 20, 40, 80 and 100 mmol/L, respectively)). The sperm motility, plasma membrane integrity, acrosome integrity, mitochondrial membrane potential, protamine deficiency, capacitation and in vitro fertilization of frozen-thawed Songliao Black pig sperm were detected. The results showed that replacing part of glycerol with glutamine could improve the quality of frozen thawed sperm, and the degree of improvement was affected by the concentration of glutamine. Compared with control group Ⅰ, the sperm quality parameters in group Ⅰ decreased significantly (P<0.05). Compared with group Ⅰ, the sperm motility, acrosome integrity and viability in group Ⅱ increased significantly (P<0.05), the sperm mitochondrial membrane potential in group Ⅲ increased significantly (P<0.05), and the sperm motility, plasma membrane integrity, acrosome integrity, viability and mitochondrial membrane potential in group Ⅴ increased significantly (P<0.05). It showed that replacing part of glycerol with glutamine had a great impact on sperm quality, and higher quality sperm could be obtained when glutamine was 100 mmol/L. Therefore, 100 mmol/L glutamine was used in subsequent experiments. Compared with control group, the sperm protamine deletion rate of 2% glycerol+100 mmol/L glutamine treatment group decreased significantly (P<0.05), there was no significant difference in sperm capacitation(P>0.05), but the cleavage rate of embryo was increased significantly (P<0.05). In conclusion, glutamine could be used as a new cryoprotectant replacing part of glycerol to improve the quality of pig semen, reduce the toxic effect of glycerol on pig semen, and provide technical support for cryopreservation and commercial production.

Key words: pig sperm; frozen-thawed; glutamine; capacitation; in vitro fertilization

CLC Number:

S828

ZHANG Yuyang, WANG Qiuyue, LYU Yanqiu, CHEN Xuan, XU Haifeng, ZHANG Zhengwen, JIN Yi. Effects of Partially Replacing Glycerol with Glutamine on Capacitation and in vitro Fertilization Ability of Frozen-thawed Pig Sperm[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4125-4132.

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Effects of Partially Replacing Glycerol with Glutamine on Capacitation and in vitro Fertilization Ability of Frozen-thawed Pig Sperm

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