藏红花素对小鼠卵母细胞体外成熟能力的影响

doi:10.16431/j.cnki.1671-7236.2021.10.025

Abstract

Abstract: This study was aimed to investigate the effects of crocin on the in vitro maturation and subsequent embryonic development of mouse oocytes. Different concentrations of crocin (0, 5, 10, 15, 20, 25, 30 μmol/L) were added to the in vitro mature (IVM) culture medium, after IVM for 12 h, the first polar body extrution, the contents of reactive oxygen species (ROS) and glutathione (GSH) in the cytoplasm of oocytes were detected and then in vitro fertilization (IVF)was performed. Fertilization rate, cleavage rate and blastocyst rate were counted at 6, 24 and 96 h after IVF, respectively. The results showed that when compared with control group, the addition of 10 and 15 μmol/L crocin significantly increased the polar body extrution rate (P<0.05), and when the concentration of crocin further increased, the polar body extrution rate decreased, 30 μmol/L crocin significantly decreased the polar body extrution rate (P<0.05). 5, 10 and 15 μmol/L crocin could significantly reduce the content of ROS (P<0.05), and 10 and 15 μmol/L crocin increased the content of GSH of oocytes (P<0.05). Compared with control group, 10 μmol/L crocin had no significant difference in fertilization rate, cleavage rate and blastocyst rate (P>0.05), 15, 20, 25 and 30 μmol/L crocin all significantly decreased fertilization rate and blastocyst rate (P<0.05), and had no significant difference on cleavage rate (P>0.05). The results indicated that the addition of 10 μmol/L crocin to mouse oocytes in vitro maturation medium could significantly increase the rate of the first polar body extrution, reduce the content of ROS, and increase the content of GSH in oocytes, but did not affect the embryonic development ability of oocytes.

Key words: crocin; oocytes; in vitro maturation; reactive oxygen species; in vitro fertilization

CLC Number:

S823.8⁺3

AIRIXIATI Dilixiati, ZHOU Zhengna, ZHANG Ye, LIU Zhiqiang, ZHANG Yafeng, ZHAO Xi, ABULIZI Wusiman. Effects of Crocin on the in vitro Maturation and Embryonic Development of Mouse Oocytes[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3734-3740.

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Effects of Crocin on the in vitro Maturation and Embryonic Development of Mouse Oocytes

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