2015－2020年天津地区猪伪狂犬病病毒gB、gC、gE基因遗传变异分析

doi:10.16431/j.cnki.1671-7236.2021.10.026

Abstract

Abstract: To understand the epidemic and genetic variation of porcine Pseudorabies virus (PRV) in Tianjin area, gB, gC and gE genes of 20 isolated strains from Tianjin during 2015 to 2020 were amplified, sequenced and analyzed with the sequences of reference strains. Similarity analysis showed that when compared with the Chinese strain after 2012, the homology of nucleotide and amino acid of the three genes were as follows:gB gene were both 99.9%-100%;gC gene were 99.7%-100% and 99.4%-100%, respectively; gE gene were 99.7%-100% and 99.6%-100%, respectively. Phylogenetic and sequence alignment analysis showed that according to gB, gC, gE genes phylogenetic trees, PRV strains could be divided into GⅠ and GⅡ genotypes, isolated strains from Tianjin were clustered in GⅡ genotype. 19 isolated strains showed a closer genetic relationship with the PRV variant strains, distributed in the same gene subtype, and had the same amino acid variation characteristic. The gB and gE genes of the other strain (TJBD6) showed a closer genetic relationship with the PRV variant strains, and the amino acid variation positions were the same as those of the PRV variant strains, however, its gC gene was closely related with the classical strain Ea, and the nucleotide and amino acid sequence similarity was 100%. These results indicated that there were two types of PRV strains prevalent in Tianjin since 2015:Classical strains and variant strains, and the variant strains were the main prevalent strains. This study preliminarily investigated the molecular epidemiological characteristics of PRV in Tianjin area, which could provide a basis for the prevention and control of pseudorabies.

Key words: Pseudorabies virus (PRV); gB gene; gC gene; gE gene; phylogenetic analysis

CLC Number:

S852.65⁺1

ZHANG Li, REN Weike, LU Chao, LI Xiuli, LI Fuqiang, TIAN Xiangxue, CHI Jingjing, WANG Lili, JIANG Shan, DONG Zhimin, YAN Minghua. Genetic Variation Analysis of gB, gC and gE Genes of Porcine Pseudorabies Viruses Isolated in Tianjin During 2015-2020[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3741-3751.

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Genetic Variation Analysis of gB, gC and gE Genes of Porcine Pseudorabies Viruses Isolated in Tianjin During 2015-2020

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