Cdc42基因对不同发育阶段无尾鸡骨骼的共表达调控

doi:10.16431/j.cnki.1671-7236.2021.11.001

Abstract

Abstract: To elucidate the potential regulatory mechanism of cell division cycle 42 (Cdc42) gene in the skeletal development of rumpless chicken, in this study, the hereditation of Cdc42 gene on tail development of rumpless chicken was revealed by genome pool resequencing and RNA sequencing, combined with genomics and bone development transcriptomics. Under the strong positive selection of the rumpless varieties, highly differentiated genes were significantly enriched in biological process such as neural development, basal metabolism and cytoskeleton rearrangement between rumpless and tailed chickens. The number of differentially expressed genes during bone development increased gradually next to decreased from the 8th, 11th and 14th day of embryo, on the 16th day it reached the lowest level and then rose again and reached the peak on the 21st day of embryo. The common candidate Cdc42 gene was identified at the genomics and transcriptomics levels. The common biological processes enriched in regulation of GTPase activity and the regulation of extent of cell growth. Common signaling pathways include tight junctions and adherens junctions. The weighted gene co-expression network analysis by differentially expressed genes showed that the density of gene network was more than 6 times higher and the average connectivity was more than 8 times higher in rumpless chicken than that of tailed chicken. The correlation between Cdc42 gene and other genes was low in the co-expression network of rumpless chicken. In contrast, Cdc42 gene was at the core of the network and acted as a bridge for the co-regulation of multiple genes in tailed chicken. In addition, Cdc42 gene was proved to be conserved among multiple species. In this study, it was finally determined that the formation of the rumpless trait in Piao chicken might co-regulate the tight junction signaling pathway through Cdc42 and its co-expressed genes, and interact with the Wnt signaling pathway, thus interfering with the development of growth plate and coccyx, and ultimately affecting the important link of premature termination of coccyx. These results would be provided theoretical guidance for the early initiation and genetic basis of rumpless phenotype.

Key words: rumpless chicken; Cdc42 gene; bone development; genome selection; co-expression gene

CLC Number:

S813

QI Wangmei, HU Sile, XU Siriguleng, HA Dengchuriya, BAI Xiaoying, ZHANG Wenguang. Co-expression Regulation of Cdc42 Gene in the Bone of Rumpless Chickens at Different Developmental Stages[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3905-3917.

References

[1] TENIN G, WRIGHT D, FERJENTSIK Z, et al. The chick somitogenesis oscillator is arrested before all paraxial mesoderm is segmented into somites[J]. BMC Developmental Biology, 2010, 10(1):1-12.
[2] NOORAI R E, FREESE N H, WRIGHT L M, et al. Genome-wide association mapping and identification of candidate genes for the rumpless and ear-tufted traits of the Araucana Chicken[J]. PLoS One, 2012, 7(7):e40974.
[3] STOTT D, KISPERT A, HERRMANN B G.Rescue of the tail defect of Brachyury mice[J]. Gene Development, 1993, 7(2):197-203.
[4] WANG Q, PI J, PAN A, et al. A novel sex-linked mutant affecting tail formation in Hongshan chicken[J]. Scientific Reports, 2017, 7(1):10079.
[5] WOODS A, WANG G, DUPUIS H, et al. Rac1 signaling stimulates N-cadherin expression, mesenchymal condensation, and chondrogenesis[J]. The Journal of Biological Chemistry, 2007, 282(32):23500-23508.
[6] AIZAWA R, YAMADA A, SEKI T, et al. Cdc42 regulates cranial suture morphogenesis and ossification[J]. Biochemical and Biophysical Research Communications, 2019, 512(2):145-149.
[7] WANG G, BEIER F.Rac1/Cdc42 and RhoA GTPases antagonistically regulate chondrocyte proliferation, hypertrophy, and apoptosis[J]. Journal of Bone and Mineral Research, 2005, 20(6):1022-1031.
[8] SUZUKI W, YAMADA A, AIZAWA R, et al. Cdc42 is critical for cartilage development during endochondral ossification[J]. Endocrinology, 2015, 156(1):314-322.
[9] IKEHATA M, YAMADA A, FUJITA K, et al. Cooperation of Rho family proteins Rac1 and Cdc42 in cartilage development and calcified tissue formation[J]. Biochemical and Biophysical Research Communications, 2018, 500(3):525-529.
[10] RUBIN C J, ZODY M C, ERIKSSON J, et al. Whole-genome resequencing reveals loci under selection during chicken domestication[J]. Nature, 2010, 464(7288):587-591.
[11] GONGPAN P C, LIU L X, LI D L, et al. The investigation of genetic diversity of Piao chicken based on mitochondrial DNA D-loop region sequence[J]. Mitochondrial DNA, 2016, 27(4):211-223.
[12] LI M, TIAN S, YEUNG C K, et al. Whole-genome sequencing of Berkshire (European native pig) provides insights into its origin and domestication[J]. Scientific Reports, 2014, 4(4):4678.
[13] FREESE N H, LAM B A, STATON M, et al. A novel gain-of-function mutation of the proneural IRX1 and IRX2 genes disrupts axis elongation in the Araucana rumpless chicken[J]. PLoS One, 2014, 9(11):e112364.
[14] CAMBRAY N, WILSON V.Axial progenitors with extensive potency are localised to the mouse chordoneural hinge[J]. Development, 2002, 129(20):4855-4866.
[15] GONG H, WANG H, WANG Y, et al. Skin transcriptome reveals the dynamic changes in the Wnt pathway during integument morphogenesis of chick embryos[J]. PLoS One, 2018, 13(1):e0190933.
[16] 胡斯乐.有尾鸡与无尾鸡比较基因组学研究[D].呼和浩特:内蒙古农业大学, 2018. HU S L.Comparative genomic analysis of tailed chicken and rumples chicken[D].Hohhot:Inner Mongolia Agricultural University, 2018.(in Chinese)
[17] ZHANG W, XU Y, ZHANG L, et al. Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development[J]. Aging Cell, 2018, 17(4):e12788.
[18] EI-SHARKAWY I, LIANG D, XU K.Transcriptome analysis of an apple (Malus×Domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation[J]. Journal of Experimental Botany, 2015, 66(22):7359-7376.
[19] SCHLESINGER P H, BLAIR H C, BEER S D, et al. Cellular and extracellular matrix of bone, with principles of synthesis and dependency of mineral deposition on cell membrane transport[J]. American Journal of Physiology, 2020, 318(1):C111-C124.
[20] ALSHBOOL F Z, MOHAN S.Emerging multifunctional roles of claudin tight junction proteins in bone[J]. Endocrinology, 2014, 155(7):2363-2376.
[21] STAINS J P, CIVITELLI R.Cell-to-cell interactions in bone[J]. Biochemical and Biophysical Research Communication, 2005, 328(3):721-727.
[22] ARANA-CHAVEZ V E, SOARES A M, KATCHBURIAN E.Junctions between early developing osteoblasts of rat calvaria as revealed by freeze-fracture and ultrathin section electron microscopy[J]. Archives of Histology and Cytology, 1995, 58(3):285-292.
[23] TEUNISSEN M, RIEMERS F M, VAN LEENEN D, et al. Growth plate expression profiling:Large and small breed dogs provide new insights in endochondral bone formation[J]. Journal of Orthopaedic Research, 2018, 36(1):138-148.
[24] NELSON W J, NUSSE R.Convergence of Wnt, beta-catenin, and cadherin pathways[J]. Science, 303(5663):1483-1487.
[25] TUAN R S.Cellular signaling in developmental chondrogenesis:N-cadherin, Wnts, and BMP-2[J]. The Journal of Bone and Joint Surgery, 2003, 2:137-141.
[26] CHO S H, OH C D, KIM S J, et al. Retinoic acid inhibits chondrogenesis of mesenchymal cells by sustaining expression of N-cadherin and its associated proteins[J]. Journal of Cellular Biochemistry, 2003, 89(4):837-847.
[27] WANG Y, LI Y P, PAULSON C, et al. Wnt and the Wnt signaling pathway in bone development and disease[J]. Frontiers in Bioence, 2014, 19(3):379-407.
[28] LANDAUER W.Recessive rumplessness of fowl with kyphoscoliosis and supernumerary ribs[J]. Genetics, 1945, 30(5):403.
[29] HALL A, NOBES C D.Rho GTPases:Molecular switches that control the organization and dynamics of the actin cytoskeleton[J]. Philosophical Transactions of the Royal Society of London, 2000, 355(1399):965-970.
[30] ETIENNE-MANNEVILLE S, HALL A.Rho GTPases in cell biology[J]. Nature, 2002, 420(6916):629-635.
[31] WANG G, WOODS A, AGOSTON H, et al. Genetic ablation of Rac1 in cartilage results in chondrodysplasia[J]. Developmental Biology, 2007, 306(2):612-623.
[32] GAO J, MA S, YANG F, et al. MiR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma[J]. Oncology Reports, 2020, 44(1):139-155.
[33] WANG Y M, KHEDERZADEH S, LI S R, et al. Integrating genomic and transcriptomic data to reveal genetic mechanisms underlying Piao chicken rumpless trait[J]. Genomics Proteomics Bioinformatics, 2021, 21:S1672.
[34] CHEN F, MA L, PARRINI M C, et al. Cdc42 is required for PIP(2)-induced actin polymerization and early development but not for cell viability[J]. Current Biology, 2000, 10(13):758-765.
[35] 胡斯乐, 陶萨如拉, 王月星, 等.鸡无尾性状的分子机制研究进展[J]. 中国畜牧兽医, 2018, 45(1):154-161. HU S L, TAO S, WANG Y X, et al. Research progress on molecular mechanisms of rumples trait in chicken[J]. China Animal Husbandry & Veterinary Medicine, 2018, 45(1):154-161.(in Chinese)

Co-expression Regulation of Cdc42 Gene in the Bone of Rumpless Chickens at Different Developmental Stages

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments