白藜芦醇对玉米赤霉烯酮致小鼠肝脏氧化损伤及炎症的保护作用

doi:10.16431/j.cnki.1671-7236.2021.11.035

Abstract

Abstract: In order to explore whether resveratrol (RSV) had a protective effect on the liver injury of mice induced by zearalenone (ZEA), 40 clean grade male BALB/c mice were randomly divided into 5 groups:Control group (normal saline), ZEA group (40 mg/kg), different concentrations of RSV (50, 100 or 200 mg/kg) and ZEA (40 mg kg) co-treatment groups, with 8 mice in each group. Each group was administered by gavage for 12 days. After the experiment, the liver samples of mice were collected, the liver coefficient was calculated, the liver pathological changes were observed by HE staining and immunohistochemical staining, and the liver NF-κB was detected. The content of malondialdehyde (MDA), the activities of superoxide dismutase (SOD) and catalase (CAT) in liver tissue were detected by colorimetry. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in serum were detected by colorimetry. The levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and IL-1β in serum were detected by ELISA method. The results showed that compared with control group, the liver tissue of ZEA group had obvious pathological changes. Compared with ZEA group, the liver histopathological changes of mice treated with different concentrations of RSV and ZEA were reduced. Immunohistochemical results showed that compared with control group, liver NF-κB protein expression was increased. Compared with ZEA group, liver NF-κB protein expression of different concentrations of RSV and ZEA co-treatment groups were decreased. Compared with control group, the liver coefficient and the content of MDA in liver tissue in ZEA group were extremely significantly increased (P<0.01), the activities of SOD and CAT in liver tissue were significantly or extremely significantly decreased (P<0.05; P<0.01), the activities of AST, ALT and LDH, and the contents of IL-6 and TNF-α and IL-1β in serum were extremely significantly increased (P<0.01). Compared with ZEA group, CAT activity in liver tissue of different concentrations of RSV and ZEA co-treatment groups were significantly or extremely significantly increased (P<0.05; P<0.01); Activities of AST, ALT and LDH and the contents of IL-6 and IL-1β in serum were significantly or extremely significantly decreased (P<0.05; P<0.01); SOD activity in liver tissue and the content of TNF-α in serum of 50 mg/kg RSV and ZEA co-treatment group were significantly increased (P<0.05) and extremely significantly decreased (P<0.01), respectively; The liver coefficient and MDA content in liver tissue were significantly or extremely significantly decreased in 100 and 200 mg/kg RSV and ZEA co-treatment groups (P<0.05; P<0.01). The results showed that ZEA had serious oxidative and inflammatory effects on the liver, and RSV had a certain protective effect on the liver injury caused by ZEA poisoning, 100 mg/kg RSV had the best protect effect.

Key words: resveratrol; zearalenone; oxidative damage; inflammation; liver

CLC Number:

S853.74

ZHU Gensheng, XIA Sugan, SHE Jinjin, BAI Yuni, ZOU Hui, GU Jianhong, YUAN Yan, LIU Xuezhong, LIU Zongping, BIAN Jianchun. Protective Effects of Resveratrol on Zearalenone-induced Liver Oxidative Damage and Inflammation in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4254-4261.

References

[1] YOON J E, LEE K Y, SEOK J S, et al. Zearalenone induces endoplasmic reticulum stress and modulates the expression of phase Ⅰ/Ⅱ enzymes in human liver cells[J]. Toxins (Basel), 2020, 12(1):2.
[2] ZOURGUI L, GOLLI E E, BOUAZIZ C, et al. Cactus (Opuntia ficus-indica) cladodes prevent oxidative damage induced by the mycotoxin zearalenone in BALB/c mice[J]. Food Chemical Toxicology, 2008, 46(5):1817-1824.
[3] SKIEPKON, PRZYBYLSKA-GORNOWICZB, GAJĘCKA M, et al. Effects of deoxynivalenol and zearalenone on the histology and ultrastructure of pig liver[J]. Toxins (Basel), 2020, 12(7):463.
[4] ZORGUI L, AYED-BOUSSEMA I, AYED Y, et al. The antigenotoxic activities of cactus (Opuntia ficus-indica) cladodes against the mycotoxin zearalenone in BALB/c mice:Prevention of micronuclei, chromosome aberrations and DNA fragmentation[J]. Food Chemical Toxicology, 2009, 47(3):662-667.
[5] 韩建鑫, 何剑斌, 高锋, 等.原花青素对玉米赤霉烯酮致小鼠肝脏、肾脏氧化损伤的保护作用[J]. 中国畜牧兽医, 2016, 43(2):402-406. HAN J X, HE J B, GAO F, et al. Protective effects of proanthocyanidins on zearalenone-induced oxidative damage of liver and kidney in mice[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(2):402-406.(in Chinese)
[6] LI S, TAN H Y, WANG N, et al. The role of oxidative stress and antioxidants in liver diseases[J]. International Molecular Science, 2015, 16(11):26087-260124.
[7] BEN SALEM I, BOUSSABBEH M, HELALI S, et al. Protective effect of Crocin against zearalenone-induced oxidative stress in liver and kidney of BALB/c mice[J]. International Environmental Science Pollution and Research, 2015, 22(23):19069-19076.
[8] SEHIRLI O, TOZAN A, OMURTAG G Z, et al. Protective effect of resveratrol against naphthalene-induced oxidative stress in mice[J]. Ecotoxicology and Environmental Safety, 2008, 71(1):301-308.
[9] PALSAMY P, SUBRAMANIAN S.Resveratrol protects diabetic kidney by attenuating hyperglycemia-mediated oxidative stress and renal inflammatory cytokines via Nrf2-Keap1 signaling[J]. Journal of Biochemistry and Biophysics, 2011, 1812(7):719-731.
[10] SANG Y, LI W, ZHANG G.The protective effect of resveratrol against cytotoxicity induced by mycotoxin, zearalenone[J]. Food and Function, 2016, 7(9):3703-3715.
[11] EDWARDS J A, BECK M, RIEGGER C, et al. Safety of resveratrol with examples for high purity, trans-resveratrol, resVida(^®)[J]. Proceedings of the New York Academy of Sciences, 2011, 1215:131-137.
[12] CHENG K, YAN E, SONG Z, et al. Protective effect of resveratrol against hepatic damage induced by heat stress in a rat model is associated with the regulation of oxidative stress and inflammation[J]. Journal of Thermal Biology, 2019, 82:70-75.
[13] OUANES Z, ABID S, AYED I, et al. Induction of micronuclei by zearalenone in Vero monkey kidney cells and in bone marrow cells of mice:Protective effect of vitamin E[J]. Mutant Research, 2003, 538(1-2):63-70.
[14] OUANES Z, AYED-BOUSSEMA I, BAATI T, et al. Zearalenone induces chromosome aberrations in mouse bone marrow:Preventive effect of 17 beta-estradiol, progesterone and vitamin E[J]. Mutant Research, 2005, 565(2):139-149.
[15] CHATOPADHYAY P, PANDEY A, CHAURASIA A K, et al. Hepatic hyperplasia and damages induces by zearalenone Fusarium mycotoxins in BALB/c mice[J]. Gastrointestinal Science, 2012, 49(1):77-81.
[16] HOU Y J, ZHAO Y Y, XIONG B, et al. Mycotoxin-containing diet causes oxidative stress in the mouse[J]. PLoS One, 2013, 8(3):e60374.
[17] GAO X, XIAO Z H, LIU M, et al. Dietary silymarin supplementation alleviates zearalenone-induced hepatotoxicity and reproductive toxicity in rats[J]. Nutrition Magazine, 2018, 148(8):1209-1216.
[18] WANG N, LI P, WANG M, et al. The protective role of Bacillus velezensis A2 on the biochemical and hepatic toxicity of zearalenone in mice[J]. Toxins (Basel), 2018, 10(11):449.
[19] VITRAC X, DESMOULIōRE A, BROUILLAUD B, et al. Distribution of[14C] -trans-resveratrol, a cancer chemopreventive polyphenol, in mouse tissues after oral administration[J]. Life Sciences, 2003, 72(20):2219-2233.
[20] 张文华, 闻双全, 王莉, 等.葛根素对镉致大鼠肝毒性损伤的保护作用[J]. 中国兽医科学, 2020, 50(10):1333-1339. ZHANG W H, WEN S Q, WANG L, et al. Protective effects of puerarin on cadmium-induced hepatotoxicity in rats[J]. Chinese Veterinary Science, 2020, 50(10):1333-1339.(in Chinese)
[21] JIANG S Z, YANG Z B, YANG W R, et al. Effects of purified zearalenone on growth performance, organ size, serum metabolites, and oxidative stress in postweaning gilts[J]. Journal of Animal Science, 2011, 89(10):3008-3015.
[22] 浩斯娜, 卓娜, 海勒, 等.骆驼尿液对预防CCl₄诱导小鼠肝损伤的保护作用[J]. 中国畜牧兽医, 2019, 46(5):1316-1322. HAO S N, ZHUO N, HAI L, et al. Protective effects of camel urine on preventing liver injury induced by CCl₄ in mice[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(5):1316-1322(in Chinese)
[23] REYES-GORDILLO K, SEGOVIA J, SHIBAYAMA M, et al. Curcumin protects against acute liver damage in the rat by inhibiting NF-kappaB, proinflammatory cytokines production and oxidative stress[J]. Journal of Biochemistry and Biophysics, 2007, 1770(6):989-996.
[24] TSAI M S, WANG Y H, LAI Y Y, et al. Kaempferol protects against propacetamol-induced acute liver injury through CYP2E1 inactivation, UGT1A1 activation, and attenuation of oxidative stress, inflammation and apoptosis in mice[J]. Toxicology Letter, 2018, 290:97-109.
[25] BEN SALAH-ABBōS J, ABBōS S, ABDEL-WAHHAB M A, et al. Raphanus sativus extract protects against zearalenone induced reproductive toxicity, oxidative stress and mutagenic alterations in male BALB/c mice[J]. Toxicon, 2009, 53(5):525-533.

Protective Effects of Resveratrol on Zearalenone-induced Liver Oxidative Damage and Inflammation in Mice

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