山羊RPL27A基因克隆及组织表达特性分析

doi:10.16431/j.cnki.1671-7236.2021.11.002

Abstract

Abstract: In order to study the gene structure and biological function of ribosome protein L27A (RPL27A) in goats, 14 kinds of tissue samples (i. e, heart, liver, spleen, longissimus dorsi muscle, subcutaneous fat, etc.) of Jianzhou Big-eared goats were collected. The sequence of RPL27A gene in goats was obtained by RT-PCR amplification and cloning, and its biological characteristics were analyzed using online tools. Real-time quantitative PCR was used to detect the expression of RPL27A gene in the collected different tissues as well as the subcutaneous preadipocytes at different differentiation stages. The results showed that the CDS region of RPL27A gene in goats was 447 bp, which encoded 148 amino acids. The bioinformatics analysis results showed that RPL27A gene was highly conserved among different species. RPL27A protein was an unstable alkalinity protein, and had obvious interactions with the ribosomal proteins such as RPS18, RPL26, RPL34, RPL32 and RPL37A, which related to lipid metabolism and fat deposition. RPL27A protein had no transmembrane domain and signal peptide, and subcellular localization indicated that it mainly existed in cytoplasm. Real-time quantitative PCR results reveled that RPL27A gene was widely expressed in 14 tissues of goats, such as heart, liver, spleen, kidney, lung, longissimus dorsi muscle and subcutaneous fat, while the expression of RPL27A gene was the highest in rumen, and the expression level in triceps brachii and biceps femoris was significantly higher than that in other tissues (P<0.05). The expression level of RPL27A gene in subcutaneous adipocytes of goats induced for 60 h was significantly higher than that before differentiation (P<0.05). In this study, the CDS sequence of RPL27A gene in goats was successfully cloned, and the expression characteristics of RPL27A gene in 14 tissues of goats were revealed, which could provide materials for elucidating the regulatory effect and mechanism of RPL27A gene on the differentiation of adipocyte in goats.

Key words: goats; RPL27A gene; cloning; tissue expression

CLC Number:

ZHENG Jianying, WANG Jinling, WANG Yong, ZHU Jiangjiang, ZHANG Hao. Cloning and Tissue Expression Characterization Analysis of RPL27A Gene in Goats[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3918-3928.

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Cloning and Tissue Expression Characterization Analysis of RPL27A Gene in Goats

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