福清山羊和戴云山羊夏季发情期卵巢组织比较转录组分析

doi:10.16431/j.cnki.1671-7236.2019.11.017

Abstract

Abstract: In order to reveal the molecular mechanism of superior fecundity of Daiyun goat in summer season and find out the useful genetic variation in ovary.Transcriptome sequencing of ovary tissue in Fuqing and Daiyun goats were performed by Illumina HiSeq.Differentially expressed genes (DEGs) were screened using gene ontology (GO),cluster of orthologous groups of proteins (COG) and kyoto encyclopedia of genes and genomes (KEGG) databases,which were verified by Real-time quantitative PCR.The results showed that there were 357 DEGs in Fuqing and Daiyun goats,221 DEGs were up-regulated and 136 DEGs were down-regulated.Cluster analysis results showed that 287,78 and 219 DEGs annotated into the GO,COG and KEGG databases,respectively.KEGG analysis results showed that 219 DEGs were mainly enriched in protein digestion and absorption,phagosome,tuberculosis,ECM-receptor interaction, Staphylococcus aureus infection and allograft rejection.Real-time quantitative PCR results showed that the expression patterns of the selected gene (transcript) were consistent with that of transcriptome sequencing,indicating that the sequencing results were reliable.In this study,a large amount of ovarian transcriptome information in goat was obtained by high-throughput sequencing,which was helpful to further study the ovary in goats at the molecular level.

Key words: Fuqing goats; Daiyun goats; ovary; transcriptome; differentially expressed genes(DEGs)

CLC Number:

S827

WU Xianfeng, LI Wenyang, LIU Yuan, ZHANG Ying, HUANG Qinlou. Comparative Transcriptome Analysis of Ovary Tissues in Fuqing and Daiyun Goats During Summer Estrus[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3277-3288.

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Comparative Transcriptome Analysis of Ovary Tissues in Fuqing and Daiyun Goats During Summer Estrus

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