高、低产双黄蛋高邮鸭卵巢组织差异分析

doi:10.16431/j.cnki.1671-7236.2018.07.022

Abstract

Abstract:

Gaoyou ducks were used as the experiment objects in this study,12 Gaoyou ducks were chosen from high and low double-yolk egg laying groups (6 ducks per group),and hypothalamus,pituitary gland,liver,fallopian tube,ovary tissues were collected immediately after bleeding to death by carotid artery cut, HE staining was made to observe the ovary morphological structure and compare the development levels of follicle in two groups.FSHR immunohistochemical staining was conducted to observe its expression in positive cells and the distribution of ovarian granulosa cells.The total RNA was extracted from the hypothalamus, pituitary gland,liver,fallopian tube and ovary tissues, Real-time PCR method was used to detect the expression of FSHR gene in these tissues.The results showed that there were significant differences in ovary structure between high and low laying Gaoyou ducks.The follicles were dense and well developed,numerous small yellow follicles, small and big white follicles were distributed, and the sizes of the follicles at all levels were significantly different in high laying Gaoyou ducks'ovary;While low laying Gaoyou ducks' ovary had less and undeveloped follicles.The results of Real-time PCR showed that the expression of FSHR gene in hypothalamus,hypophysis gland, liver and fallopian tube of high laying ducks were extremely significantly higher than those in the low laying ducks (P<0.01), but there were no significant difference in ovary between two groups (P>0.05).In conlusion,the ovary structure and follicle development of high and low laying Gaoyou ducks had significant differences,and the microenvironment in the ovary might affect double-yolk egg laying performance of Gaoyou duck.

Key words: Gaoyou duck; double-yolk egg; ovary; ovarian granulosa cells; FSHR

CLC Number:

S834

ZHANG Lei, ZHANG Xiangying, ZHANG Jingqi, DONG Biao, ZHANG Yang, CHANG Guobin, CHEN Guohong, WU Bingxin, WANG Jian. Differential Analysis of Ovary Structure Between High and Low Double-yolk Egg Laying Gaoyou Duck[J]. , 2018, 45(7): 1905-1911.

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Differential Analysis of Ovary Structure Between High and Low Double-yolk Egg Laying Gaoyou Duck

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