活禽产业链中大肠杆菌耐药性及耐药基因调查

doi:10.16431/j.cnki.1671-7236.2021.07.043

Abstract

Abstract: To understand the proportion of antimicrobial resistance and antimicrobial resistance genes of Escherichia coli from different links of the live poultry production chain in the family farm supply mode,in this study,132 samples were collected from one farmers' market and 33 family farms in Yantan town,Zigong city.Methods of bacteria isolation and purification,morphological examination,specific PCR detection,phylogenetic group detection,drug sensitivity test and antimicrobial resistance genes detection were used for the analysis of bacterial characteristics.The results showed that 83 isolates were E.coli strains which were black and metallic lustre colonies in EMB,Gram-negative bacteria under microscope and positive in specific primer test.Phylogenetic group detection results showed that 83 strains were concentrated in Group A (53.02%) and Group B1 (20.48%).The results of drug resistance test showed that 83 strains were multi-drug resistant bacteria,among which proportion drug resistance of colstin and tetracycline were 100% and proportion drug resistance of ampicillin,cefuroxime,aztreonam and fluorobenzene were over 90%.The results of antimicrobial resistance genes detection showed that antimicrobial resistance genes such as mcr-1, mcr-3, bla_TEM, bla_SHV, bla_{CTX-M-group 1}, bla_{CTX-M-group 9}, qnrS, aac(6')-Ⅰb-cr, tetA and tetB were detected and proportion of these genes were lower than proportion of antimicrobial resistance.In addition,the analysis results showed that proportion of antimicrobial resistance and antimicrobial resistance genes of E.coli from family farm was lower than that from farmers' market.This study proved that farmers' market had the aggregation function of antimicrobial resistance bacterial and antimicrobial resistance genes in live poultry production chain.This study provided experiment basis for the detection,diagnosis and treatment of avian Escherichia coli.As the same time,the study provided reliable data to assess transmission risk of antimicrobial resistant in live poultry production chain.

Key words: live poultry production chain; Escherichia coli; phylogeny groups; antimicrobial resistance; antimicrobial resistance genes

CLC Number:

S852.61⁺2

YUAN Jingzhi, YANG Liyao, CHEN Siyu, CAI Zongling, LI Xun, LIAO Yuying, WANG Xiaoye. Investigation of Antimicrobial Resistance and Antimicrobial Resistance Genes of E.coli from the Live Poultry Production Chain[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2661-2670.

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Investigation of Antimicrobial Resistance and Antimicrobial Resistance Genes of E.coli from the Live Poultry Production Chain

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