东佛里生羊MSTN基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.12.022

Abstract

Abstract: This study was aimed to explore the polymorphism of myostatin (MSTN) gene and its effect on the growth traits in East Friensian sheep.DNA was extracted from a total of 324 East Friensian sheep, PCR amplification was performed on the exon sequence and UTR of MSTN gene.SNP were identified by the first-generation sequencing technology and its genetic diversity parameters were analyzed.At the same time, the association analysis between genotypes and growth traits at different loci was carried out.The results showed that no mutation was detected in exon of MSTN gene in East Friensian sheep, but there was a G→A single-base mutation at 272 bp in 3'-UTR region, which contained AG and AA genotypes.The frequency of AA genotype was higher than that of AG genotype.A mutation of C→A was detected at 176 bp of 5'-UTR, which contained CC and AC genotypes, and the frequency of CC genotype was higher than that of AC genotype.The results of genetic diversity parameter analysis showed that the homozygosity of 3'-UTR-272 and 5'-UTR-176 of MSTN gene in East Friensian sheep were 0.827 and 0.887, and the heterozygosity were 0.173 and 0.113, respectively.The polymorphisms of the two loci were low (PIC<0.25), and the genotype distribution accorded with Hardy-Weinberg equilibrium (P>0.05).Through association analysis, the body oblique length of AG genotype was significantly higher than that of AA genotype at 3'-UTR-272 (P<0.05), the chest circumference of CC genotype was significantly higher than AC genotype at 5'-UTR-176 (P<0.05), and there was no significant difference in other traits (P>0.05).This results suggested that the 3'-UTR and 5'-UTR mutation of MSTN gene in East Friensian sheep could be considered as molecular markers for the selection of target traits.

Key words: East Friensian sheep; MSTN gene; polymorphism; growth traits; association analysis

CLC Number:

S813.3

ZHANG Lei, LI Danni, WANG Yuchen, YANG Lichun, HE Jing, SONG Yuxuan. Polymorphism of MSTN Gene and Its Association with Growth Traits in East Friensian Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4537-4544.

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Polymorphism of MSTN Gene and Its Association with Growth Traits in East Friensian Sheep

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