不同乳脂率德宏奶水牛乳腺组织脂滴形成相关基因的表达研究

doi:10.16431/j.cnki.1671-7236.2021.12.023

Abstract

Abstract: The purpose of this study was to explore the expression differences of BTNA1A1、XDH、PLIN1 and PLIN2 genes which were lipid droplet formation related genes in the mammary tissue of Dehong dairy buffalo with different milk fat contents.216 healthy Dehong dairy buffaloes with the same breeding community were selected in this experiment.Milk samples were collected, and milk fat contents were detected by milk composition analyzer.According to the determination result of milk fat contents, the experiments were divided into low milk fat content group (L group, <7.0%), medium milk fat content group (M group, 7.5%±0.5%) and high milk fat content group (H group, >8.0%).Three Dehong dairy buffaloes with second fetuses, similar age and in the middle lactation stage were selected in each group.After slaughter, the breast tissue was collected and the total RNA was extracted.The mRNA expression levels of lipid droplet formation related genes in mammary tissue were detected by Real-time quantitative PCR, and the correlation between the lipid droplet formation related genes expression and milk fat contents, fatty acid contents were analyzed.The results showed that the same expression trend of BTN1A1, XDH, PLIN1 and PLIN2 genes were fallow as:group H>group M>group L.The mRNA expression levels of BTN1A1, XDH, PLIN1 and PLIN2 genes in the mammary tissue of Dehong dairy buffalo in group H were extremely significantly higher than those in group M and group L (P<0.01).The expression level of BTN1A1 gene was no significant difference between group M and group L (P>0.05), but the mRNA expression levels of XDH, PLIN1 and PLIN2 genes in group M were extremely significantly higher than those in group L (P<0.01).Correlation analysis showed that the expression levels of BTN1A1, XDH, PLIN1 and PLIN2 genes were significantly positively correlated with milk fat contents, total fatty acids, long chain fatty acids and unsaturated fatty acids contents (P<0.01).The results of this study indicated that the expression of BTN1A1, XDH, PLIN1 and PLIN2 in mammary tissue might have a positive effect on milk fat synthesis and secretion in Dehong dairy buffalo, which would provide a reference for further explanation of regulation mechanisms on lipid droplet formation related genes during lactation.

Key words: Dehong dairy buffalo; mammary tissue; lipid droplet formation related genes; gene expression

CLC Number:

S823.8⁺3

TANG Na, JIANG Juncai, WANG Mengdi, CHA Xuedian, ZHANG Yongyun, YANG Zhong, LI Weizhen. Expression of Genes Related to Lipid Droplet Formation in Mammary Tissue of Dehong Dairy Buffalo with Different Milk Fat Contents[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4545-4553.

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Expression of Genes Related to Lipid Droplet Formation in Mammary Tissue of Dehong Dairy Buffalo with Different Milk Fat Contents

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