绵羊附睾褪黑素受体1的表达与定位

doi:10.16431/j.cnki.1671-7236.2018.09.026

Abstract

Abstract:

In order to study the expression pattern of melatonin receptor 1 (MT1) in epididymis of sheep at different age,epididymis were collected from lamb (2 to 3 months old),young ram (6 to 8 months old) and adult sheep (2 to 3 years old),and the expression of MT1 mRNA and the distribution of MT1 in different parts of the epididymis were detected by the Real-time quantitative PCR and immunohistochemistry.The results showed that the MT1 transcription level in the lambs cauda was extremely significantly higher than that in the caput and corpus (P<0.01).The MT1 transcription level in corpus and cauda of young ram were significantly higher than that in the caput (P<0.05).The transcription level of MT1 in adult ram cauda was significantly higher than that in the caput and corpus (P<0.05).The transcription level of MT1 in epididymal decreased with age.The immunohistochemistry results showed that MT1 could be detected in all parts of sheep epididymis and mainly distributed in the epididymal epithelial cells.It was concluded that MT1 were expressed and distributed in different parts of the epididymis of sheep at different age,and the MT1 expression in epididymis decreased with age.The MT1 expression level was higher in cauda than other parts of epididymis for sheep at the same age.

Key words: sheep; epididymis; melatonin receptor 1(MT1); gene expression; protein localization

CLC Number:

S814.8

SONG Huizi, BIAN Xiaona, LI Ruilan, LIU Chunjie, ZHANG Jiaxin. Expression and Localization of Melatonin Receptor 1 in Sheep Epididymis[J]. , 2018, 45(9): 2544-2549.

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Expression and Localization of Melatonin Receptor 1 in Sheep Epididymis

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