RBP4基因在绵羊性成熟前后附睾中表达的研究

doi:10.16431/j.cnki.1671-7236.2020.03.019

Abstract

Abstract: The purpose of this study was to investigate the expression of retinol binding protein 4 (RBP4) gene in epididymis of sheep before and after sexual maturity and its role in the regulation of reproductive function.The expression of RBP4 gene mRNA in epididymis before and after sexual maturity was detected by Real-time quantitative PCR,and the expression of RBP4 gene in epididymis head and epididymis tail before and after sexual maturity was detected by immunohistochemistry.The results showed that the expression level of RBP4 gene was 1.3368 and 0.6450 before and after sexual maturity,respectively.Although there was no significant difference between them (P>0.05),the expression level before sexual maturity was higher than that after sexual maturity.RBP4 protein was expressed in the epididymal cortex,smooth muscle layer and interstitial tissue before and after sexual maturity,mainly in the epithelial cytoplasm and smooth muscle cytoplasm,and in the epididymal cortex and interstitial tissue,mainly in the epithelial cytoplasm.It was suggested that RBP4 gene might be related to the secretory function of epididymal main cells and the contractile function of epididymal head,indicating that RBP4 might participate in the regulation of epididymal microenvironment,which was conducive to sperm maturation,movement and storage.

Key words: sheep; RBP4 gene; epididymis; retinol-binding protein; reproductive performance; gene expression

CLC Number:

S826.9

HAN Yue, LIU Hang, CHEN Yang, JIANG Huaizhi. Expression of RBP4 Gene in Epididymis Before and After Sexual Maturation in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 807-813.

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Expression of RBP4 Gene in Epididymis Before and After Sexual Maturation in Sheep

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