绵羊FRZB基因InDel检测及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2020.03.018

Abstract

Abstract: In this study,FRZB gene was selected as a candidate gene,582 sheep were selected as experimental materials from four breeds of Tong sheep,Small-tailed Han sheep,Lanzhou Big-tailed sheep and Hu sheep.DNA pool sequencing combined with agarose gel electrophoresis was used to screen the InDel of FRZB gene in order to find the sites of InDel related to the growth traits of four sheep breeds.The results showed that a 14 bp InDel mutation was detected in the first intron of FRZB gene in sheep;The InDel mutation site in the intron region of FRZB gene had three genotypes (II,ID and DD) in Tong Sheep,Small-tailed Han sheep,Lanzhou Big-tailed sheep and Hu sheep populations;Association analysis showed that the InDel mutation detected in FRZB gene had significant effects on the growth traits in Tong sheep.The body length,back height,hip height,talus width,tail length and tail width of II genotype were significantly superior to DD genotype in Tong sheep (P<0.05),it could be used as a candidate molecular marker for the molecular marker-assisted selection of Tong sheep.These results suggested that a novel 14 bp insertion mutation in the first intron of FRZB gene in sheep could be significantly affecting the growth trait of Tong sheep,and could be used as a potential molecular marker for growth traits in sheep breeding.

Key words: sheep; FRZB gene; insertion/deletion; growth traits; association analysis

CLC Number:

S813.3

CHEN Pingbo, ZHAO Haidong, WU Mingli, WEI Yanpei, SUN Xiuzhu, WANG Shuhui. Detection of InDel of FRZB Gene and Its Association Analysis with Growth Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 799-806.

References

[1] HOANG B,MOOS M,VUKICEVIC S,et al.Primary structure and tissue distribution of FRZB,a novel protein related to drosophila frizzled,suggest a role in skeletal morphogenesis[J].The Journal of Biological Chemistry,1996,271(42):26131-26137.
[2] TAPIA-ROJAS C,SCHULLER A,LINDSAY C B,et al.Andrographolide activates the canonical Wnt signalling pathway by a mechanism that implicates the non-ATP competitive inhibition of GSK-3beta:autoregulation of GSK-3beta in vivo[J].Biochemical Journal,2015,466(2):415-430.
[3] ZHANG M,LI Y,RAO P,et al.Blockade of receptors of advanced glycation end products ameliorates diabetic osteogenesis of adipose-derived stem cells through DNA methylation and Wnt signalling pathway[J].Cell Proliferation,2018,51(5):e12471.
[4] RESS A,MOELLING K.Bcr is a negative regulator of the Wnt signalling pathway[J].Embo Reports,2005,6(11):1095-1100.
[5] WANG S,KRINKS M,LIN K,et al.Frzb,a secreted protein expressed in the spemann organizer,binds and inhibits Wnt-8[J].Cell,1997,88(6):757-766.
[6] LEYNS L,BOUWMEESTER T,KIM S H,et al.Frzb-1 is a secreted antagonist of Wnt signaling expressed in the spemann organizer[J].Cell,1997,88(6):747-756.
[7] PERSON A D,GARRIOCK R J,KRIEG P A,et al.FRZB modulates Wnt-9a-mediated beta-catenin signaling during avian atrioventricular cardiac cushion development[J].Developmental Biology,2005,278(1):35-48.
[8] WANG Z,LI Q,ZHANG B,et al.Single nucleotide polymorphism scanning and expression of the FRZB gene in pig populations[J].Gene,2014,543(2):198-203.
[9] 杨华,徐珍,陈佳,等.猪FRZB、ABCF1基因多态性与生长性状的关联分析[J].中国畜牧杂志,2018,54(1):38-41. YANG H,XU Z,CHEN J,et al.Genetic polymorphism of FRZB gene and ABCF1 and their association with growth traits in pigs[J].Chinese Journal of Animal Science,2018,54(1):38-41.(in Chinese)
[10] THYSEN S,CAILOTTO F,LORIES R.Osteogenesis induced by frizzled-related protein (FRZB) is linked to the netrin-like domain[J].Laboratory Investigation,2016,96(5):570-580.
[11] FOGARTY N M.A review of the effects of the booroola gene (FecB) on sheep production[J].Small Ruminant Research,2009,85(2):75-84.
[12] LI W,CHENG J,WU Z,et al.An InDel-based linkage map of hot pepper (Capsicum annuum)[J].Molecular Breeding,2015,35(1):32.
[13] 邬明丽,李世鹏,高源,等.黄牛LYST基因InDel检测及其与生长性状的关联分析[J].家畜生态学报,2019,40(2):21-26. WU M L,LI S P,GAO Y,et al.Identification of the InDel in LYST gene and its genetic effect on Chinese cattle[J].Journal of Domestic Animal Ecology,2019,40(2):21-26.(in Chinese)
[14] 武卫影.多重PCR快速检测乳品中三种病原菌方法研究[D].保定:河北农业大学,2012. WU W Y.Multiplex PCR assay for rapid detection of there bacterial pathogens in dairy food[D].Baoding:Hebei Agricultural University,2012.(in Chinese)
[15] 王燕新,廖圆圆,蔡勇,等.三个绵羊品种LHX3基因多态性与生长性状的关联分析[J].生物技术通报,2019,35(10):162-168. WANG Y X,LIAO Y Y,CAI Y,et al.Correlation analysis between LHX3 gene polymorphism and growth traits in Three sheep breeds[J].Biotechnology Bulletin,2019,35(10):162-168.(in Chinese)
[16] 刘波,陈宏,蓝贤勇,等.秦川牛及其杂种牛POU1F1基因多态与生长性能相关性[J].中国农业科学,2005,12:2520-2525. LIU B,CHEN H,LAN X Y,et al.Correlation between polymorphism of POU1F1 gene and growth traits in Qinchuan cattle and its hybrid cattle[J].Scientia Agricultura Sinica,2005,12:2520-2525.(in Chinese)
[17] WANG J,GAO J,LIU C,et al.High precision slope deformation monitoring model based on the GPS/Pseudolites technology in open-pit mine[J].Mining Science and Technology,2010,20(1):126-132.
[18] ANGERS S,MOON R T.Proximal events in Wnt signal transduction[J].Nature Reviews Molecular Cell Biology,2009,10(7):468-477.
[19] PARK J R,JUNG J W,LEE Y S,et al.The roles of Wnt antagonists Dkk1 and sFRP4 during adipogenesis of human adipose tissue-derived mesenchymal stem cells[J].Cell Proliferation,2008,41(6):859-874.
[20] 杨韩,张阳海,王敏,等.陕北白绒山羊POU1F1基因3'-UTR多态性及其与生长性状的相关分析[J].农业生物技术学报,2019,27(7):1224-1232. YANG H,ZHANG Y H,WANG M,et al.Polymorphisms of 3'-UTR of POU1F1 gene and its association with growth traits in Shaanbei white cashmere goats (Capra hircus)[J].Journal of Agricultural Biotechnology,2019,27(7):1224-1232.(in Chinese)
[21] 王志秀,李庆岗,杨玉增,等.淮猪新品系猪中FRZB基因与生长性状相关位点分析[J].中国畜牧杂志,2014,50(7):11-13. WANG Z X,LI Q G,YANG Y Z,et al.Gene loci of FRZB related to growth trait in new-line Huai pig population[J].Chinese Journal of Animal Science,2014,50(7):11-13.(in Chinese)
[22] MINOR E A,COURT B L,SANDER D,et al.AMD-associated variants at the chromosome 10q26 locus and the stability of ARMS2 transcripts[J].Investigative Ophthalmology & Visual Science,2013,54(8):5913-5919.
[23] MOSE L E,HAYES D N,PEROU C M,et al.Improved indel detection in RNA-seq data via assembly based re-alignment reveals expressed epidermal growth factor receptor indels in lung adenocarcinoma[J].Cancer Research,2017,77(13 Supplement):3592.
[24] ZOPPOLI G,DOUARRE C,DALLA R I,et al.Coordinated regulation of mitochondrial topoisomerase IB with mitochondrial nuclear encoded genes and MYC[J].Nucleic Acids Research,2011,39(15):6620-6632.
[25] ZHAO H D,HE S,WANG S,et al.Two new insertion/deletion variants of the PITX2 gene and their effects on growth traits in sheep[J].Animal Biotechnology,2018,29(4):276-282.

Detection of InDel of FRZB Gene and Its Association Analysis with Growth Traits in Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Saiqiao, ZHAO Lu, ZHAI Zhenhan, ZHANG Binglei, JIA Wanhang, WANG Yuqin. Tissue Expression and Bioinformatics Analysis of miR-449a/b Precursor Sequences in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2175-2184.
[2]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[3]	LIU Jinyu, ZHANG Yang, MA Xumei, ZHOU Qian, ZHOU Yang, ZHANG Chunxiang, SHI Lei, REN Youshe. Effects of Glutathione on Cryopreservation of Sheep Semen [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2388-2394.
[4]	ZHANG Huaqiang, WANG Pinsheng, LI Xiao, WEI Panpeng, YAN Lingmin, JIANG Bingbing, WANG Zhaogui, WANG Xuefang, ZENG Huanqing, FAN Yi, GAO Zhenglong, WANG Xuebing. Effects of Fermented Hybrid Broussonetia papyrifera on Growth Performance, Meat Quality,Digestive Enzyme Activity and Intestinal Flora Structure of Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1876-1887.
[5]	ZHAO Qianming, QI Meng, XU Zeli, GONG Pihong, LU Chenyang, PEI Zhiyang, JIAN Fuchun, WANG Jiandong, JING Bo, NING Changsheng. Investigation of Intestinal Parasites Occurrence in Sheep and Environmental Contamination in Large-scale Housing Farms [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2156-2165.
[6]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[7]	CUI Shuang, Wurlig, Alder, XIAO Min, WANG Jianqiu, GONG Jian. Effects of Yeast Selenium Supplementation on Gastrointestinal Microflora in Sheep Under Grazing Conditions [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 952-965.
[8]	ZHANG Yongjie, SUI Zhiyuan, ZHANG Zhishuai, WANG Chenguang, LI Xiaojun, XING Feng. Cloning and Bioinformatics Analysis of NPTX1 Gene and Its Tissues Expression at Puberty in Dolang Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1048-1058.
[9]	ZHANG Mingliang, ZHANG Hailong, MA Shipeng, WANG Cailian, LANG Xia. Effects of Zanthoxylum bungeanum Essential Oil on Production Performance and Gastrointestinal Tissue Structure of Small-tail Han Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 556-563.
[10]	LI Chenglan, GUO Tingting, YUE Yaojing. Research Advances on Genomic Selection and Its Application in Sheep Breeding [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 616-625.
[11]	QIN Zhiyun, JIANG Huaizhi. An Overview of Research on Skin Hair Follicle Development and Regulatory Mechanism in Sheep During the Fetal Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 638-646.
[12]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[13]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[14]	AN Xiaopeng, LIU Nan, HU Zhengjiamei, HU Jincao, XU Xiaolong, ZHANG Lei, SONG Yuxuan. Comparative Analysis of Lactation Performance of East Friesen Sheep, Hu Sheep and East Friesen-Hu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 238-245.
[15]	LIU Gang, ZHU Fangxian, ZHANG Xiaowei, GU Feng, LIU Lijun, SU Rui, MENG Fei, XU Haitao, SUN Feizhou. Evaluation of Embryo and Sperm Cryopreservation in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3492-3499.